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    Detection of human papillomavirus mRNA in cervical smears
    2.
    发明授权
    Detection of human papillomavirus mRNA in cervical smears 失效
    宫颈涂片中人乳头状瘤病毒mRNA的检测

    公开(公告)号：US5750334A

    公开(公告)日：1998-05-12

    申请号：US479567

    申请日：1995-06-07

    申请人： Peter Cerutti ,  Jeannette Whitcomb ,  Jacob Zijlstra ,  Ethel-Michelle de Villiers

    发明人： Peter Cerutti ,  Jeannette Whitcomb ,  Jacob Zijlstra ,  Ethel-Michelle de Villiers

    IPC分类号： G01N33/569 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/92 ,  G01N33/574 ,  C12P19/34

    CPC分类号： C12Q1/708 ,  C12Q1/686

    摘要： The invention relates to the direct detection of human papillomavirus MRNA and DNA. The recently developed polymerase chain reaction was modified in order to improve the sensitivity and specificity. By choosing appropriate oligonucleotide primers (amplimers) and reaction temperatures, individual HPV genes from among the total cell DNA can be identified and amplified to an extent such that non-isotopic detection is possible. In addition, use of reverse transcription permits selective amplification of spliced mRNA and thus permits indication of premalignant or malignant conditions and/or lesions.

    摘要翻译： 本发明涉及人乳头瘤病毒MRNA和DNA的直接检测。 最近开发的聚合酶链反应被修改以提高敏感性和特异性。 通过选择适当的寡核苷酸引物（扩增片段）和反应温度，可以鉴定和扩增来自总细胞DNA的单个HPV基因，使得非同位素检测成为可能。 此外，使用逆转录允许对拼接的mRNA进行选择性扩增，从而允许恶变或恶性病症和/或病变的征兆。

    Device for preparing a beverage suitable for human consumption with a fine-bubble foam layer
    3.
    发明申请
    Device for preparing a beverage suitable for human consumption with a fine-bubble foam layer 有权
    用于制备适合人类消费的饮料的装置，其具有细泡沫泡沫层

    公开(公告)号：US20070079707A1

    公开(公告)日：2007-04-12

    申请号：US10581352

    申请日：2004-12-10

    申请人： Joeke Noordhuis ,  Jacob Zijlstra

    发明人： Joeke Noordhuis ,  Jacob Zijlstra

    IPC分类号： B67D1/00

    CPC分类号： A47J31/467 ,  A47J31/0668 ,  A47J31/4496

    摘要： The invention relates to a device (1) for preparing a beverage suitable for human consumption and having a fine-bubble foam layer. The device is provided with a beverage unit (2) for delivering the beverage under pressure and at least one nozzle (4) which is in fluid communication with the beverage unit for supplying the beverage to the nozzle such that the nozzle can generate a jet (12) of beverage. The device further comprises a receiving unit (14) into which the jet is directed for obtaining the beverage with the fine-bubble foam layer. The receiving unit is provided with a chamber (16) with at least one drain opening (18) for delivering the beverage with the fine-bubble foam layer and a jet impact member (20) accommodated in the chamber and having a top (22) that lies free from an inner wall (24) of the chamber. The nozzle and the jet impact member are mutually oriented such that-the jet hits against a portion of the top of the jet impact member, so that the jet after hitting against the jet impact member forms a mist of the beverage. The beverage with the fine-bubble foam layer subsequently leaves the drain opening. The device is constructed such that air (34) can only be supplied to the chamber through the at least one drain opening and/or through an air supply channel which extends through the jet impact member into the chamber.

    摘要翻译： 本发明涉及一种用于制备适合人类消费的饮料并具有细泡沫泡沫层的装置（1）。 该装置设置有用于在压力下输送饮料的饮料单元（2）和与饮料单元流体连通的至少一个喷嘴（4），用于将饮料供应到喷嘴，使得喷嘴可以产生喷嘴（ 12）饮料。 所述装置还包括接收单元（14），所述射流被引导到所述接收单元（14）以获得具有所述细泡沫泡沫层的饮料。 所述接收单元设置有具有至少一个用于将所述饮料与所述微小泡沫泡沫层一起输送的至少一个排放开口（18）的腔室（16），以及容纳在所述腔室中并具有顶部（22）的射流冲击构件（20） 其离开室的内壁（24）。 喷嘴和喷射冲击构件相互取向，使得喷射器撞击喷射冲击构件的顶部的一部分，使得喷射冲击喷射冲击构件之后的射流形成饮料的雾。 具有细泡沫泡沫层的饮料随后离开排水开口。 该装置被构造成使得空气（34）仅能够通过至少一个排放开口和/或通过穿过喷射冲击构件延伸到室中的空气供应通道供应到室。