公开(公告)号：US5367474A

公开(公告)日：1994-11-22

申请号：US014701

申请日：1993-02-08

申请人： Robert E. Auer ,  Bruce M. Weber ,  John D. Starling ,  James C. S. Wood

发明人： Robert E. Auer ,  Bruce M. Weber ,  John D. Starling ,  James C. S. Wood

IPC分类号： G01N21/64 ,  G01J1/44 ,  G01N15/00 ,  G01N15/14 ,  G01N21/49 ,  G01N33/48 ,  G06F17/40 ,  G06F19/00

CPC分类号： G01N15/14 ,  G01J2001/4406 ,  G01N15/1456

摘要： A flow cytometer includes a plurality of detectors for providing voltage pulse signals over a four decade range as particles pass through an illuminated detection station. Each voltage pulse signal is processed and provided to a first sample and hold for storage. Thereafter, the value in the first sample and hold is amplified by one or thirty-two, depending upon its magnitude, and stored in a second sample and hold for subsequent provision to a sixteen bit analog to digital convertor (ADC), one signal at a time. The first sample and hold is then free to store a new value while the old value is awaiting provision to the ADC. The ADC converts the signal received from the second sample and hold to a fifteen bit digital signal and uses the sixteenth bit to manifest whether the signal stored in the second sample and hold was amplified by thirty-two.

摘要翻译： 流式细胞仪包括多个检测器，用于在粒子通过照明检测站时在四十年的范围内提供电压脉冲信号。 每个电压脉冲信号被处理并提供给第一采样并保持用于存储。 此后，取决于其大小，将第一采样和保持中的值放大一到三十二分，并存储在第二采样中并保持以供随后提供给十六位模数转换器（ADC），一个信号在 一次 然后，第一个采样和保持可以自由存储新值，而旧值正在等待提供给ADC。 ADC将从第二个样本接收的信号转换为十五位数字信号，并使用第十六位来显示存储在第二个采样和保持中的信号是否放大了三十二个。

公开(公告)号：US5451525A

公开(公告)日：1995-09-19

申请号：US836638

申请日：1992-02-14

申请人： Mark L. Shenkin ,  Ronald M. Hamelik ,  James C. S. Wood

发明人： Mark L. Shenkin ,  Ronald M. Hamelik ,  James C. S. Wood

IPC分类号： G01N33/49 ,  G01N15/00 ,  G01N15/02 ,  G01N15/14 ,  G01N33/50 ,  G01N33/569 ,  G01N33/48

CPC分类号： G01N33/5094 ,  G01N33/56972 ,  G01N2015/1477 ,  G01N2015/1486

摘要： A method is provided to ascertain the total number of cells per unit volume of a cell specimen in an apparatus which does not measure the volume of a suspension containing the specimen as it is being analyzed. The method includes mixing a suspension of a known quantity of particles having a first light scatter signal with a known volume of a cell specimen having a second light scatter signal different from the first light scatter signal to obtain a suspension having a concentration of particles per specimen volume, passing each of said particles and each cell in said cell specimen, in turn, through a light beam, each of said particles and cells producing at least one forward light scattering pattern, counting the number of cells and the number of particles in a portion of said suspension, and determining the total number of cells per unit volume of the specimen. In a preferred embodiment of the invention, the method further includes correlating the light scattering pattern and fluorescence signal of the particles to a predetermined ratio which represents a threshold of minimum instrument performance and adjusting, if the predetermined ratio has not been attained, the operation of said instrument, while the particles are within the incident beam of light, until the measured ratio reaches said predetermined ratio whereby the instrument is analyzing both particles and cells in said cell suspension.

摘要翻译： 提供了一种方法，以确定在不分析包含样品的悬浮液的体积的装置中每单位体积的细胞样本的总细胞数。 该方法包括将具有第一光散射信号的已知量的具有第一光散射信号的颗粒的悬浮液与具有不同于第一光散射信号的第二光散射信号的已知体积的细胞样本混合以获得具有每个样品的颗粒浓度的悬浮液 体积，使所述细胞样本中的所述颗粒和每个细胞依次通过光束，每个所述颗粒和细胞产生至少一种正向光散射图案，计数细胞数量和颗粒数量 并且确定样品的每单位体积的总细胞数。 在本发明的一个优选实施例中，该方法还包括将颗粒的光散射图案和荧光信号与表示最小仪器性能的阈值的预定比例相关联，并且如果还没有达到预定比例，则调节 所述仪器，同时所述颗粒在所述入射光束内，直到所述测量的比率达到所述预定比率，由此所述仪器正在分析所述细胞悬浮液中的颗粒和细胞。