公开(公告)号：US5830458A

公开(公告)日：1998-11-03

申请号：US487776

申请日：1995-06-07

Applicant: Harry E. Gruber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

Inventor： Harry E. Gruber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

IPC: A01K67/027 ,  A61K38/00 ,  A61K38/19 ,  A61K38/20 ,  A61K38/21 ,  A61K39/00 ,  A61K39/21 ,  A61K48/00 ,  C07K14/155 ,  C07K14/16 ,  C07K14/73 ,  C12N7/04 ,  C12N9/12 ,  C12N15/867

CPC classification number: A01K67/0275 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/21 ,  C07K14/005 ,  C07K14/70514 ,  C12N15/86 ,  C12N7/00 ,  C12N9/1211 ,  A01K2217/05 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  A61K48/00 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13032 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/16023 ,  C12N2740/16134 ,  C12N2740/16222

Abstract: Recombinant retroviruses carrying a vector construct capable of preventing, inhibiting, stabilizing or reversing infectious, cancerous or auto-immune diseases are disclosed. More specifically, the recombinant retroviruses of the present invention are useful for (a) stimulating a specific immune response to an antigen or a pathogenic antigen; (b) inhibiting a function of a pathogenic agent, such as a virus; and (c) inhibiting the interaction of an agent with a host cell receptor. In addition, eucaryotic cells infected with, and pharmaceutical compositions containing such a recombinant retrovirus are disclosed. Various methods for producing recombinant retroviruses having unique characteristics, and methods for producing transgenic packaging animals or insects are also disclosed.

Abstract translation: 公开了携带能够预防，抑制，稳定或逆转感染性，癌性或自身免疫性疾病的载体构建体的重组逆转录病毒。 更具体地，本发明的重组逆转录病毒可用于（a）刺激对抗原或病原性抗原的特异性免疫应答; （b）抑制病原体如病毒的功能; 和（c）抑制药剂与宿主细胞受体的相互作用。 此外，公开了感染的真核细胞和含有这种重组逆转录病毒的药物组合物。 还公开了用于生产具有独特特征的重组逆转录病毒的各种方法，以及用于生产转基因包装动物或昆虫的方法。

公开(公告)号：US06333195B1

公开(公告)日：2001-12-25

申请号：US09479776

申请日：2000-01-07

Applicant: James G. Respess ,  Nicholas J. DePolo ,  Sunil Chada ,  Sybille Sauter ,  Mordechai Bodner ,  David A. Driver

Inventor： James G. Respess ,  Nicholas J. DePolo ,  Sunil Chada ,  Sybille Sauter ,  Mordechai Bodner ,  David A. Driver

IPC: C12N15867

CPC classification number: C12N15/86 ,  C12N2740/13043 ,  C12N2740/13052

Abstract: Retroviral vector constructs are described which have a 5′ LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3′ LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gag/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.

Abstract translation: 描述了具有5'LTR，tRNA结合位点，包装信号，一个或多个异源序列，第二链合成起始和3'LTR的逆转录病毒载体构建体，其中载体构建体缺乏逆转录病毒gag / pol或env 编码序列。 此外，描述了gag / pol和env表达盒，其中表达盒缺少共同超过8个核苷酸的连续序列。 上述逆转录病毒载体构建体，gag / pol和env表达盒可用于构建排除形成复制能力的病毒的生产细胞系。

公开(公告)号：US6013517A

公开(公告)日：2000-01-11

申请号：US850961

申请日：1997-05-05

Applicant: James G. Respess ,  Nicholas J. DePolo ,  Sunil Chada ,  Sybille Sauter ,  Mordechai Bodner ,  David A. Driver

Inventor： James G. Respess ,  Nicholas J. DePolo ,  Sunil Chada ,  Sybille Sauter ,  Mordechai Bodner ,  David A. Driver

IPC: C12N15/48 ,  C12N15/867 ,  C12N5/10 ,  C12N15/86

CPC classification number: C12N15/86 ,  C12N2740/13043 ,  C12N2740/13052

Abstract: Retroviral vector constructs are described which have a 5' LTR, a tRNA binding site, a packaging signal, one or more heterologous sequences, an origin of second strand synthesis and a 3' LTR, wherein the vector construct lacks retroviral gag/pol or env coding sequences. In addition, gag/pol, and env expression-cassettes are described wherein the expression cassettes lack a consecutive sequence of more than 8 nucleotides in common. The above-described retroviral vector constructs, gag/pol and env expression cassettes may be utilized to construct producer cell lines which preclude the formation of replication competent virus.

Abstract translation: 描述了具有5'LTR，tRNA结合位点，包装信号，一个或多个异源序列，第二链合成起始和3'LTR的逆转录病毒载体构建体，其中该载体构建体缺乏逆转录病毒gag / pol或env 编码序列。 此外，描述了gag / pol和env表达盒，其中表达盒缺少多于8个核苷酸的连续序列。 上述逆转录病毒载体构建体，gag / pol和env表达盒可用于构建排除形成复制能力的病毒的生产细胞系。

公开(公告)号：US5856185A

公开(公告)日：1999-01-05

申请号：US472109

申请日：1995-06-07

Applicant: Harry E. Gruber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind ,  Jack R. Barber ,  Daniel C. St. Louis ,  Sunil D. Chada ,  Stephen M. W. Chang ,  John F. Warner

Inventor： Harry E. Gruber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind ,  Jack R. Barber ,  Daniel C. St. Louis ,  Sunil D. Chada ,  Stephen M. W. Chang ,  John F. Warner

IPC: A01K67/027 ,  A01K67/033 ,  A61K38/00 ,  A61K38/20 ,  A61K38/21 ,  A61K39/00 ,  A61K39/21 ,  A61K48/00 ,  C07K14/15 ,  C07K14/155 ,  C07K14/16 ,  C07K14/73 ,  C12N5/10 ,  C12N7/04 ,  C12N9/12 ,  C12N15/85 ,  C12N15/867 ,  C07H21/04 ,  C12N15/48

CPC classification number: A01K67/033 ,  A61K38/2013 ,  A61K38/217 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/21 ,  C07K14/005 ,  C07K14/70514 ,  C12N15/8509 ,  C12N15/86 ,  C12N7/00 ,  C12N9/1211 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  A61K35/13 ,  A61K39/00 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/13023 ,  C12N2740/13032 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234

Abstract: Recombinant retroviruses carrying a vector construct capable of preventing, inhibiting, stabilizing or reversing infectious, cancerous or auto-immune diseases are disclosed. More specifically, the recombinant retroviruses of the present invention are useful for (a) stimulating a specific immune response to an antigen or a pathogenic antigen; (b) inhibiting a function of a pathogenic agent, such as a virus; and (c) inhibiting the interaction of an agent with a host cell receptor. In addition, eucaryotic cells infected with, and pharmaceutical compositions containing such a recombinant retrovirus are disclosed. Various methods for producing recombinant retroviruses having unique characteristics, and methods for producing transgenic packaging animals or insects are also disclosed.

Abstract translation: 公开了携带能够预防，抑制，稳定或逆转感染性，癌性或自身免疫性疾病的载体构建体的重组逆转录病毒。 更具体地，本发明的重组逆转录病毒可用于（a）刺激对抗原或病原性抗原的特异性免疫应答; （b）抑制病原体如病毒的功能; 和（c）抑制药剂与宿主细胞受体的相互作用。 此外，公开了感染的真核细胞和含有这种重组逆转录病毒的药物组合物。 还公开了用于生产具有独特特征的重组逆转录病毒的各种方法，以及用于生产转基因包装动物或昆虫的方法。

公开(公告)号：US5691177A

公开(公告)日：1997-11-25

申请号：US460996

申请日：1995-06-05

Applicant: Harry E. Guber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

Inventor： Harry E. Guber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

IPC: A01K67/027 ,  A61K38/00 ,  A61K38/19 ,  A61K38/20 ,  A61K38/21 ,  A61K39/00 ,  A61K39/21 ,  A61K48/00 ,  C07K14/155 ,  C07K14/16 ,  C07K14/73 ,  C12N7/04 ,  C12N9/12 ,  C12N15/867 ,  C12N5/08 ,  C12N5/10

CPC classification number: A01K67/0275 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/21 ,  C07K14/005 ,  C07K14/70514 ,  C12N15/86 ,  C12N7/00 ,  C12N9/1211 ,  A01K2217/05 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  A61K48/00 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13032 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/16023 ,  C12N2740/16134 ,  C12N2740/16222

Abstract: Recombinant retroviruses carrying a vector construct capable of preventing, inhibiting, stabilizing or reversing infectious, cancerous or auto-immune diseases are disclosed. More specifically, the recombinant retroviruses of the present invention are useful for (a) stimulating a specific immune response to an antigen or a pathogenic antigen; (b) inhibiting a function of a pathogenic agent, such as a virus; and (c) inhibiting the interaction of an agent with a host cell receptor. In addition, eucaryotic cells infected with, and pharmaceutical compositions containing such a recombinant retrovirus are disclosed. Various methods for producing recombinant retroviruses having unique characteristics, and methods for producing transgenic packaging animals or insects are also disclosed.

Abstract translation: 公开了携带能够预防，抑制，稳定或逆转感染性，癌性或自身免疫性疾病的载体构建体的重组逆转录病毒。 更具体地，本发明的重组逆转录病毒可用于（a）刺激对抗原或病原性抗原的特异性免疫应答; （b）抑制病原体如病毒的功能; 和（c）抑制药剂与宿主细胞受体的相互作用。 此外，公开了感染的真核细胞和含有这种重组逆转录病毒的药物组合物。 还公开了用于生产具有独特特征的重组逆转录病毒的各种方法，以及用于生产转基因包装动物或昆虫的方法。

公开(公告)号：US5888502A

公开(公告)日：1999-03-30

申请号：US463122

申请日：1995-06-05

Applicant: Harry E. Guber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

Inventor： Harry E. Guber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

IPC: A01K67/027 ,  A61K38/00 ,  A61K38/19 ,  A61K38/20 ,  A61K38/21 ,  A61K39/00 ,  A61K39/21 ,  A61K48/00 ,  C07K14/155 ,  C07K14/16 ,  C07K14/73 ,  C12N7/04 ,  C12N9/12 ,  C12N15/867 ,  C12N5/10 ,  C12N15/86

CPC classification number: A01K67/0275 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/21 ,  C07K14/005 ,  C07K14/70514 ,  C12N15/86 ,  C12N7/00 ,  C12N9/1211 ,  A01K2217/05 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  A61K48/00 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13032 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/16023 ,  C12N2740/16134 ,  C12N2740/16222

Abstract: Recombinant retroviruses carrying a vector construct capable of preventing, inhibiting, stabilizing or reversing infectious, cancerous or auto-immune diseases are disclosed. More specifically, the recombinant retroviruses of the present invention are useful for (a) stimulating a specific immune response to an antigen or a pathogenic antigen; (b) inhibiting a function of a pathogenic agent, such as a virus; and (c) inhibiting the interaction of an agent with a host cell receptor. In addition, eucaryotic cells infected with, and pharmaceutical compositions containing such a recombinant retrovirus are disclosed. Various methods for producing recombinant retroviruses having unique characteristics, and methods for producing transgenic packaging animals or insects are also disclosed.

Abstract translation: 公开了携带能够预防，抑制，稳定或逆转感染性，癌性或自身免疫性疾病的载体构建体的重组逆转录病毒。 更具体地，本发明的重组逆转录病毒可用于（a）刺激对抗原或病原性抗原的特异性免疫应答; （b）抑制病原体如病毒的功能; 和（c）抑制药剂与宿主细胞受体的相互作用。 此外，公开了感染的真核细胞和含有这种重组逆转录病毒的药物组合物。 还公开了用于生产具有独特特征的重组逆转录病毒的各种方法，以及用于生产转基因包装动物或昆虫的方法。

公开(公告)号：US5716613A

公开(公告)日：1998-02-10

申请号：US474736

申请日：1995-06-07

Applicant: Harry E. Guber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

Inventor： Harry E. Guber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

IPC: A01K67/027 ,  A61K38/00 ,  A61K38/19 ,  A61K38/20 ,  A61K38/21 ,  A61K39/00 ,  A61K39/21 ,  A61K48/00 ,  C07K14/155 ,  C07K14/16 ,  C07K14/73 ,  C12N7/04 ,  C12N9/12 ,  C12N15/867 ,  C12N15/86

CPC classification number: A01K67/0275 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/21 ,  C07K14/005 ,  C07K14/70514 ,  C12N15/86 ,  C12N7/00 ,  C12N9/1211 ,  A01K2217/05 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  A61K48/00 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13032 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/16023 ,  C12N2740/16134 ,  C12N2740/16222

Abstract: Recombinant retroviruses carrying a vector construct capable of preventing, inhibiting, stabilizing or reversing infectious, cancerous or auto-immune diseases are disclosed. More specifically, the recombinant retroviruses of the present invention are useful for (a) stimulating a specific immune response to an antigen or a pathogenic antigen; (b) inhibiting a function of a pathogenic agent, such as a virus; and (c) inhibiting the interaction of an agent with a host cell receptor. In addition, eucaryotic cells infected with, and pharmaceutical compositions containing such a recombinant retrovirus are disclosed. Various methods for producing recombinant retroviruses having unique characteristics, and methods for producing transgenic packaging animals or insects are also disclosed.

公开(公告)号：US5591624A

公开(公告)日：1997-01-07

申请号：US156789

申请日：1993-11-23

Applicant: Jack R. Barber ,  Douglas J. Jolly ,  James G. Respess ,  Stephen M. W. Chang

Inventor： Jack R. Barber ,  Douglas J. Jolly ,  James G. Respess ,  Stephen M. W. Chang

IPC: A01K67/027 ,  A01K67/033 ,  A61K38/00 ,  A61K38/19 ,  A61K38/20 ,  A61K38/21 ,  A61K39/00 ,  A61K48/00 ,  C07K14/15 ,  C07K14/155 ,  C07K14/16 ,  C07K14/73 ,  C12N5/10 ,  C12N7/04 ,  C12N15/85 ,  C12N15/867 ,  C12N5/08

CPC classification number: A01K67/0275 ,  A01K67/033 ,  A61K39/0011 ,  C07K14/005 ,  C07K14/70514 ,  C12N15/8509 ,  C12N15/86 ,  C12N7/00 ,  A01K2217/05 ,  A01K2217/30 ,  A01K2227/105 ,  A01K2267/01 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/11043 ,  C12N2740/11052 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/16023 ,  C12N2740/16222 ,  C12N2830/008

Abstract: The invention described herein allows the production of recombinant retroviruses (retroviral vector particles) from producer cells which are safer and of higher titer than normal. In addition, methods are provided for making helper cells which, when a recombinant retrovirus genome is introduced to make a producer line, produce particles that are targeted toward particular cell types. Methods are also provided for making recombinant retrovirus systems adapted to infect a particular cell type, such as a tumor, by binding the retrovirus or recombinant retrovirus in the particular cell type. Methods are also provided for producing recombinant retroviruses which integrate in a specific small number of places in the host genome, and for producing recombinant retroviruses from transgenic animals.

Abstract translation: 本文所述的本发明允许从生产细胞产生重组逆转录病毒（逆转录病毒载体颗粒），其比较常规更安全并且效价更高。 此外，提供了制备辅助细胞的方法，当引入重组逆转录病毒基因组以制备生产线时，产生靶向特定细胞类型的颗粒。 还提供了通过结合特定细胞类型的逆转录病毒或重组逆转录病毒来制备适于感染特定细胞类型（例如肿瘤）的重组逆转录病毒系统的方法。 还提供了用于产生重组逆转录病毒的方法，所述重组逆转录病毒整合在宿主基因组的特定少量位置中，并用于从转基因动物产生重组逆转录病毒。

公开(公告)号：US06495349B1

公开(公告)日：2002-12-17

申请号：US08462512

申请日：1995-06-05

Applicant: Harry E. Gruber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

Inventor： Harry E. Gruber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

IPC: C12N15867

CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/21 ,  A61K48/00 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  C07K14/005 ,  C07K14/70514 ,  C12N7/00 ,  C12N9/1211 ,  C12N15/86 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13032 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/16023 ,  C12N2740/16134 ,  C12N2740/16222

Abstract: Recombinant retroviruses carrying a vector construct capable of preventing, inhibiting, stabilizing or reversing infectious, cancerous or auto-immune diseases are disclosed. More specifically, the recombinant retroviruses of the present invention are useful for (a) stimulating a specific immune response to an antigen or a pathogenic antigen; (b) inhibiting a function of a pathogenic agent, such as a virus; and (c) inhibiting the interaction of an agent with a host cell receptor. In addition, eucaryotic cells infected with, and pharmaceutical compositions containing such a recombinant retrovirus are disclosed. Various methods for producing recombinant retroviruses having unique characteristics, and methods for producing transgenic packaging animals or insects are also disclosed.

Abstract translation: 公开了携带能够预防，抑制，稳定或逆转感染性，癌性或自身免疫性疾病的载体构建体的重组逆转录病毒。 更具体地，本发明的重组逆转录病毒可用于（a）刺激对抗原或病原性抗原的特异性免疫应答; （b）抑制病原体如病毒的功能; 和（c）抑制药剂与宿主细胞受体的相互作用。 此外，公开了感染的真核细胞和含有这种重组逆转录病毒的药物组合物。 还公开了用于生产具有独特特征的重组逆转录病毒的各种方法，以及用于生产转基因包装动物或昆虫的方法。

公开(公告)号：US6133029A

公开(公告)日：2000-10-17

申请号：US479672

申请日：1995-06-06

Applicant: Harry E. Gruber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

Inventor： Harry E. Gruber ,  Douglas J. Jolly ,  James G. Respess ,  Paul K. Laikind

IPC: A01K67/027 ,  A01K67/033 ,  A61K38/00 ,  A61K38/20 ,  A61K38/21 ,  A61K39/00 ,  A61K39/21 ,  A61K48/00 ,  C07K14/15 ,  C07K14/155 ,  C07K14/16 ,  C07K14/73 ,  C12N5/10 ,  C12N7/04 ,  C12N9/12 ,  C12N15/85 ,  C12N15/867

CPC classification number: A01K67/033 ,  A61K38/2013 ,  A61K38/217 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/21 ,  C07K14/005 ,  C07K14/70514 ,  C12N15/8509 ,  C12N15/86 ,  C12N7/00 ,  C12N9/1211 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/57 ,  A61K35/13 ,  A61K39/00 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/13023 ,  C12N2740/13032 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/15022 ,  C12N2740/16134 ,  C12N2740/16222 ,  C12N2740/16234

Abstract: Recombinant retroviruses carrying a vector construct capable of preventing, inhibiting, stabilizing or reversing infectious, cancerous or auto-immune diseases are disclosed. More specifically, the recombinant retroviruses of the present invention are useful for (a) stimulating a specific immune response to an antigen or a pathogenic antigen; (b) inhibiting a function of a pathogenic agent, such as a virus; and (c) inhibiting the interaction of an agent with a host cell receptor. In addition, eucaryotic cells infected with, and pharmaceutical compositions containing such a recombinant retrovirus are disclosed. Various methods for producing recombinant retroviruses having unique characteristics, and methods for producing transgenic packaging animals or insects are also disclosed.

Abstract translation: 公开了携带能够预防，抑制，稳定或逆转感染性，癌性或自身免疫性疾病的载体构建体的重组逆转录病毒。 更具体地，本发明的重组逆转录病毒可用于（a）刺激对抗原或病原性抗原的特异性免疫应答; （b）抑制病原体如病毒的功能; 和（c）抑制药剂与宿主细胞受体的相互作用。 此外，公开了感染的真核细胞和含有这种重组逆转录病毒的药物组合物。 还公开了用于生产具有独特特征的重组逆转录病毒的各种方法，以及用于生产转基因包装动物或昆虫的方法。