公开(公告)号：US20050032088A1

公开(公告)日：2005-02-10

申请号：US10787267

申请日：2004-02-25

申请人： Veronique Dartois ,  James Hoch ,  Fernando Valle ,  Manoj Kumar

发明人： Veronique Dartois ,  James Hoch ,  Fernando Valle ,  Manoj Kumar

IPC分类号： C07K14/26 ,  C12N15/54 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/10 ,  C12P7/60

CPC分类号： C07K14/26

摘要： The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.

摘要翻译： 本发明提供了编码具有2,5-DKG通透酶活性的多肽的分离的核酸分子及其寡核苷酸。 分离的核酸分子可以在适当的细菌细胞中表达以增强2-KLG的产生，随后将其转化为抗坏血酸。 还提供了具有2,5-DKG通透酶活性的分离的多肽，其免疫原性肽及其特异的抗体。 本发明还提供鉴定新型2,5-DKG通透酶的方法。

公开(公告)号：US20070298474A1

公开(公告)日：2007-12-27

申请号：US11879260

申请日：2007-07-16

申请人： Manoj Kumar ,  Fernando Valle ,  Veronique Dartois ,  James Hoch

发明人： Manoj Kumar ,  Fernando Valle ,  Veronique Dartois ,  James Hoch

IPC分类号： C12P7/02

CPC分类号： C12P7/60

摘要： A method for enhancing a host cell's biosynthetic production of 2-KLG is described. Such method comprises selecting a host cell that has an at least partially intracellular synthetic pathway which utilizes 2,5-DKG to produce 2-KLG; increasing the transport of said 2,5-DKG into said host cell while maintaining the integrity of the host cell; culturing the host cell to produce said 2,5-DKG; and producing 2-KLG. The transport of the 2,5-DKG is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the 2,5-DKG into the host cell.

摘要翻译： 描述了增强宿主细胞2-KLG生物合成生产的方法。 这种方法包括选择具有至少部分细胞内合成途径的宿主细胞，其利用2,5-DKG产生2-KLG; 增加所述2,5-DKG进入所述宿主细胞的同时保持宿主细胞的完整性; 培养宿主细胞以产生所述2,5-DKG; 并生产2-KLG。 通过将编码一种或多种将2,5-DKG转运到宿主细胞的酶的宿主细胞DNA转化成2,5-DKG的转运来增加。

公开(公告)号：US5848260A

公开(公告)日：1998-12-08

申请号：US566032

申请日：1995-12-01

申请人： Hudong Chen ,  David Lawerence Hill ,  James Hoch ,  Kim Molvig ,  Christopher M. Teixeira ,  Kenneth R. Traub

发明人： Hudong Chen ,  David Lawerence Hill ,  James Hoch ,  Kim Molvig ,  Christopher M. Teixeira ,  Kenneth R. Traub

IPC分类号： G06F17/50 ,  G06F19/00 ,  G06Q50/00 ,  G06T15/40

CPC分类号： G06T15/40 ,  G06F17/5018 ,  G06F2217/16

摘要： To simulate physical processes, state vectors for each of multiple voxels are stored in a memory along with a representation for each of multiple facets that are sized and oriented independently of the size and orientation of the voxels and, in combination, represent one or more surfaces. Each state vector includes multiple entries, each of which corresponds to a number of elements at a particular momentum state of multiple possible momentum states at a voxel. Interaction operations that model interactions between elements of different momentum states are performed on the state vectors, and surface interaction operations that model interactions between a facet and elements at one or more voxels near the facet are performed on the representations of facets. Finally, move operations that reflect movement of elements to new voxels are performed on the state vectors.

摘要翻译： 为了模拟物理过程，将多个体素中的每一个的状态矢量与存储器一起存储在存储器中，以及与体素的尺寸和取向无关的大小和取向的多个面的表示，并且组合地表示一个或多个表面 。 每个状态向量包括多个条目，每个条目对应于在体素处的多个可能动量状态的特定动量状态下的多个元素。 在状态向量上执行模拟不同动量状态的元素之间的交互的交互操作，并且在小平面的表示上执行在小面附近的一个或多个体素之间建模小面与元素之间的交互的表面交互操作。 最后，在状态向量上执行将元素的移动反映到新体素的操作。

公开(公告)号：US20060147950A1

公开(公告)日：2006-07-06

申请号：US11223258

申请日：2005-09-09

申请人： James Hoch ,  Veronique Dartois

发明人： James Hoch ,  Veronique Dartois

IPC分类号： C40B40/08

CPC分类号： G01N33/502 ,  C12N15/1034 ,  C12Q1/18 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/5014 ,  G01N33/5038

摘要： The invention provides methods of identifying a host cell that encodes a metabolic pathway that converts a precursor molecule into a growth inhibitory compound, by: (a) culturing a population of host cells under conditions that allow expression of the metabolic pathway; (b) contacting the host cells, or an extract thereof, with a population of target cells and the precursor molecule; and (c) identifying a host cell that inhibits growth of the target cells in the presence, but not in the absence, of the precursor molecule, where an identified host cell from step (c) contains a metabolic pathway that converts the precursor molecule into a growth inhibitory compound. Methods of identifying host cells that encode a metabolic pathway that converts a precursor molecule into a desired product compound are also provided. Further. provided are methods of identifying nucleic acid molecules encoding the metabolic pathways.

摘要翻译： 本发明提供了鉴定编码代谢途径的宿主细胞的方法，所述代谢途径通过以下方式将前体分子转化成生长抑制化合物：（a）在允许代谢途径表达的条件下培养宿主细胞群; （b）使宿主细胞或其提取物与靶细胞群和前体分子接触; （c）鉴定在前体分子的存在但不存在的情况下抑制靶细胞生长的宿主细胞，其中来自步骤（c）的鉴定的宿主细胞含有将前体分子转化为 生长抑制化合物。 还提供了鉴定编码将前体分子转化为所需产物化合物的代谢途径的宿主细胞的方法。 进一步。 提供了鉴定编码代谢途径的核酸分子的方法。

公开(公告)号：US06368793B1

公开(公告)日：2002-04-09

申请号：US09172952

申请日：1998-10-14

申请人： James Hoch ,  Veronique Dartois

发明人： James Hoch ,  Veronique Dartois

IPC分类号： C12Q168

CPC分类号： C12N15/52 ,  C12Q1/6897

摘要： The present invention relates in part to methods for screening for novel enzymatic pathways in environmental samples using metabolic selection strategies, and the isolation of the genes and proteins that make up these pathways.

摘要翻译： 本发明部分涉及使用代谢选择策略筛选环境样品中新的酶途径的方法，以及构成这些途径的基因和蛋白质的分离。