公开(公告)号：US06297018B1

公开(公告)日：2001-10-02

申请号：US09494407

申请日：2000-01-28

申请人： Todd E. French ,  Douglas N. Modlin ,  John C. Owicki ,  James S. Richey ,  Lev J. Leytes ,  Enal S. Razvi

发明人： Todd E. French ,  Douglas N. Modlin ,  John C. Owicki ,  James S. Richey ,  Lev J. Leytes ,  Enal S. Razvi

IPC分类号： C02Q168

CPC分类号： G01N21/6445 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00529 ,  B01J2219/00605 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01L3/50853 ,  B01L9/523 ,  B01L2300/0829 ,  B82Y15/00 ,  C07K7/06 ,  C07K7/08 ,  C12Q1/485 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6858 ,  C40B60/14 ,  G01N21/76 ,  G01N35/028 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/00237 ,  G01N2035/0405 ,  G01N2035/0425 ,  G01N2035/0462 ,  G01N2333/9121 ,  C12Q2565/107 ,  C12Q2561/119

摘要： Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) allele-specific hybridization, using luminescence detection, (2) allele-specific oligonucleotide ligation, using dye-labeled oligonucleotide ligation with luminescence resonance energy transfer (FRET) detection, and (3) allele-specific nucleotide incorporation, using primer extension with luminescence polarization (FP) detection. More specifically, the methods may include (1) locating a sample containing a nucleic acid material at an examination site, (2) illuminating the sample, (3) detecting light transmitted from the sample, and (4) determining the presence, absence, and/or identity of a nucleic acid target in the sample using the light transmitted from the sample.