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公开(公告)号：US4693984A
公开(公告)日：1987-09-15
申请号：US925452
申请日：1986-10-31
CPC分类号： G01N1/28 , Y10T436/2575
摘要： An apparatus for sequentially fractionating a centrifuge tube includes a capillary tube and a means for applying positive pressure. The capillary tube has an O-ring at the lower end thereof. As the capillary tube is placed within the centrifuge tube, the O-ring forms a seal within the tube. Movement of the capillary tube within the centrifuge tube places the liquid in the centrifuge tube under pressure, thus forcing the liquid to flow up through the capillary tube and into a chamber. A chase fluid is then pumped horizontally through the chamber to force the liquid therein through an exit port and into a fraction collector. The apparatus and method of the present invention may be entirely automated and controlled by a single microprocessor.
摘要翻译： 用于依次分级离心管的装置包括毛细管和用于施加正压的装置。 毛细管在其下端具有O形环。 当毛细管放置在离心管内时，O形环在管内形成密封。 离心管内的毛细管的运动将液体置于离心管内的压力下，从而迫使液体向上流过毛细管并进入腔室。 然后将追踪流体水平地泵送通过室，以迫使其中的液体通过出口并进入馏分收集器。 本发明的装置和方法可以完全自动化并由单个微处理器控制。
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公开(公告)号：US07355697B2
公开(公告)日：2008-04-08
申请号：US10926405
申请日：2004-08-26
申请人： Edward L. Mertz , James V. Sullivan
发明人： Edward L. Mertz , James V. Sullivan
IPC分类号： G01N33/20
CPC分类号： G01N21/0332 , G01N21/05 , G01N2021/0346
摘要： Optical cells are non-actively compensated to ensure that a sample gap of a sample space remains nearly constant upon a change in temperature. Fluids can be flowed through the sample space of the optical cells.
摘要翻译： 光电池不被积极地补偿，以确保样品空间的样品间隙在温度变化时保持几乎恒定。 流体可以流过光学单元的样品空间。
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公开(公告)号：US07091035B2
公开(公告)日：2006-08-15
申请号：US10334565
申请日：2002-12-30
申请人： Rea Ravin , James V. Sullivan , Ronald D. McKay
发明人： Rea Ravin , James V. Sullivan , Ronald D. McKay
IPC分类号： C12M3/00
CPC分类号： B01L3/508 , B01L2200/025 , C12M41/14 , C12M41/36 , G01N21/05 , G01N2021/0346 , G02B21/34
摘要： Featured is a long-term cell culture system being configured and arranged so as to be capable of monitoring the dynamic processes that occur during proliferation and differentiation of stem cells such as central nervous system (CNS) stem cells/embryonic stem cells. In particular aspects, the system allows monitoring of such dynamic processes continually and to focally manipulate the cells by focal application of growth factors such as BMP, CNTF and other growth factors. Preferred systems are capable of electrical recording from the cells.
摘要翻译： 特征是长期细胞培养系统的配置和排列，以便能够监测在诸如中枢神经系统（CNS）干细胞/胚胎干细胞的干细胞的增殖和分化过程中发生的动态过程。 在特定方面，该系统允许通过诸如BMP，CNTF和其他生长因子的生长因子的局部应用来连续监测这样的动态过程并且通过焦点应用细胞来控制细胞。 优选的系统能够从细胞进行电记录。
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公开(公告)号：US06309818B1
公开(公告)日：2001-10-30
申请号：US09496134
申请日：2000-02-01
IPC分类号： C12Q100
CPC分类号： G01N33/50
摘要： An apparatus having at least one template opening for causing a substantially reproducible injury to a cell, organ or tissue layer. The apparatus is useful in determining the effects of cell growth and migration agents in model wounds produced in the cell, organ or tissue layer. Also provided are methods of using the apparatus including juxtapositioning the apparatus to cells on a cell growth substrate and disrupting cells thereon.
摘要翻译： 具有至少一个模板开口的装置，用于对细胞，器官或组织层造成基本上可再现的损伤。 该装置可用于确定细胞生长和迁移剂在细胞，器官或组织层中产生的模型伤口中的作用。 还提供了使用该装置的方法，包括将该装置并置到细胞生长底物上的细胞并破坏其上的细胞。
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公开(公告)号：US5388580A
公开(公告)日：1995-02-14
申请号：US932842
申请日：1992-08-19
CPC分类号： A61B5/0555 , G01R33/34046
摘要： A head holder with the following characteristics: it is compatible with MR (no metallic parts); it is adjustable for use with adults and children; it does not produce any degradation of imaging or spectroscopy raw data; it does not induce claustrophobia; it can be used as a fixation device for cervical spine MR examinations; and it can operate with any MR unit. A strap is placed around the head of the patient at the forehead, is tightened, and then the head with band attached is positioned for optimal comfort and examining advantage. Lock screws are then tightened, holding the head comfortably in three-dimensional space. Only a single band is placed over the forehead of the person and the rest of the fixation is provided by adjusting and locking screws located in the base and along the sides of the holder. This prevents the patient from being able to rotate his or her head from side to side or up and down. The holder is designed to fit into the MR table. This is a major improvement over existing devices in that it provides for patient comfort while providing adequate head support and fixation.
摘要翻译： 具有以下特点的头架：与MR兼容（无金属部件）; 它可调节用于成人和儿童; 它不会产生成像或光谱原始数据的任何降解; 它不会引起幽闭恐惧症; 它可以用作颈椎MR检查的固定装置; 并且可以与任何MR单元一起使用。 将带子放置在患者头部的额头附近，被紧固，然后带有附接带的头部被定位以获得最佳舒适度和检查优点。 然后拧紧锁定螺钉，将头部舒适地保持在三维空间中。 只有一个单一的带放置在人的前额上，其余的固定是通过调整和锁定位于基座和沿着保持器的侧面的螺钉来提供的。 这防止患者能够将他或她的头部从一侧到另一侧或上下旋转。 支架设计成适合MR表格。 这是对现有设备的一个重大改进，因为它提供了患者舒适度，同时提供足够的头部支撑和固定。
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6.发明授权Automated system for determining the molecular weight and/or concentration of macromolecules via sedimentation equilibrium 失效用于通过沉降平衡测定大分子的分子量和/或浓度的自动化系统
公开(公告)号：US4609991A
公开(公告)日：1986-09-02
申请号：US515169
申请日：1983-07-19
申请人： Allen P. Minton , Arun K. Attri , James V. Sullivan , Paul Fitze
发明人： Allen P. Minton , Arun K. Attri , James V. Sullivan , Paul Fitze
CPC分类号： G01N15/04 , G01N2015/045 , G01N2021/0162 , G01N2021/5969
摘要： An automated system for measuring the concentration gradient of centrifuged solute and directly calculating the molecular weight and/or sedimentation coefficient of an optically absorbing solute. Monochromatic light passes through a stationary slit and a previously centrifuged sample in a tube while the tube is moved vertically at a uniform rate by a platform which is driven by a motor and gearing, including a gear nut which drives a vertical screw attached to the platform. The transmitted light impinges on a photodetector which provides absorbance readings. This provides data of absorbance vs. time through the scanning cycle, which is processed digitally. A microcomputer is used to calculate the molecular weight and/or sedimentation coefficient from this data. The centrifuge tube is supported in a transparent cuvette containing a black plastic adaptor block which conformably receives the tube and supports it in upright position, allowing the light beam to pass through opposite vertical slot portions in the block. The cuvette rests on the platform, which is moved vertically relative to the slit defining the fixed beam path. A microswitch is operated at the upper limit of travel to stop the drive motor. The motor is external to the housing in which the optical components and the sample tube are located. The external motor is coupled by a flexible shaft to the drive gearing. Alternatively, a stepping motor may be employed instead of a synchronous motor to move the centrifuge tube in an incremental fashion rather than at uniform velocity.
摘要翻译： 一种用于测量离心溶质的浓度梯度并直接计算光学吸收溶质的分子量和/或沉降系数的自动化系统。 单色光通过固定狭缝和先前离心的样品在管中，同时通过由马达和齿轮传动的平台以均匀的速度垂直移动，包括驱动附接到平台的垂直螺钉的齿轮螺母 。 透射光照射到提供吸光度读数的光电检测器上。 这提供了通过数字处理的扫描周期的吸光度对时间的数据。 使用微电脑从该数据计算分子量和/或沉降系数。 离心管支撑在透明比色杯中，该透明比色杯内装有一个黑色的塑料适配器块，它适合地接收管并将其支撑在直立位置，允许光束通过块中的相对的垂直狭槽部分。 试管座搁置在平台上，该平台相对于限定固定光束路径的狭缝垂直移动。 微动开关在行程上限运行，停止驱动电机。 电机位于壳体的外部，光学元件和样品管所在的位置。 外部电动机通过柔性轴联接到驱动齿轮传动装置。 或者，可以采用步进电机代替同步电动机，以递增的方式而不是以均匀的速度移动离心管。
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7.发明授权Flow-through, inlet-gas-temperature-controlled, solvent-resistant, thermal-expansion compensated cell for light spectroscopy 有权用于光谱的流通，入口 - 气体温度控制，耐溶剂，热膨胀补偿电池公开(公告)号：US07894055B2
公开(公告)日：2011-02-22
申请号：US11826806
申请日：2007-07-18
申请人： Edward L. Mertz , James V. Sullivan
发明人： Edward L. Mertz , James V. Sullivan
IPC分类号： G01N33/20
CPC分类号： G01N21/05 , G01N21/0332 , G01N2021/0346
摘要： Optical cells include a spacer formed of a hydrocarbon-resistant polymer, so that fluids such as hydrocarbons and alcohols can be introduced to a sample space, or include a fluid inlet and a heated inlet tube, so that a humid gas can be introduced to the sample space without condensation occurring. Optical cells can be used with, for example, solid, gel, and liquid samples. Measurements can be performed with various selected sample gaps of an optical cell.
摘要翻译： 光电池包括由耐烃聚合物形成的间隔物，使得诸如烃和醇的流体可以被引入到样品空间中，或者包括流体入口和加热的入口管，使得可以将湿气引入到 样品空间不发生冷凝。 光学细胞可以与例如固体，凝胶和液体样品一起使用。 可以用光学单元的各种选择的样本间隙进行测量。
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公开(公告)号：US07276367B2
公开(公告)日：2007-10-02
申请号：US10967703
申请日：2004-10-14
申请人： Rea Ravin , James V. Sullivan , Daniel Hoeppner , David W. Munno , Ron McKay
发明人： Rea Ravin , James V. Sullivan , Daniel Hoeppner , David W. Munno , Ron McKay
IPC分类号： C12M1/00
CPC分类号： C12M41/36 , G01N33/5029 , Y10S436/807 , Y10T83/0304
摘要： An apparatus and process for monitoring migratory cell proliferation with restricted migration on a substrate includes providing a substrate, coating the substrate with extracellular matrix, plating cells suspended in cell culture media on extracellular matrix, and placing intersecting channels across the extracellular matrix components by removing the extracellular matrix components from the channels to isolate islands of the extracellular matrix components on the substrate. When the cells are immersed with a fluid, migration of the cells is confined to the isolated islands of the extracellular matrix components, permitting long-term observation of a migratory population.
摘要翻译： 用于通过限制性迁移在基质上监测迁移细胞增殖的装置和方法包括提供底物，用细胞外基质涂覆底物，将细胞悬浮于细胞培养基中的细胞悬浮于细胞外基质上，以及通过除去细胞外基质组分 来自通道的细胞外基质组分以分离底物上细胞外基质组分的岛。 当细胞浸入液体时，细胞的迁移被限制在细胞外基质组分的孤立岛上，从而允许长期观察迁移群体。
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公开(公告)号：US5170286A
公开(公告)日：1992-12-08
申请号：US656326
申请日：1991-02-19
IPC分类号： G02B21/34
CPC分类号： G02B21/34
摘要： A specimen chamber comprising a central chamber element sandwiched between a base plate and a cover plate, which base plate and cover plate secure cover slips within recessed areas on opposite surfaces of the central chamber portion which face the base and cover plate. The central chamber element includes a central flow passage, which terminates at opposite ends into tee channels. The sandwiched arrangement of the elements is secured by locking pins, which are insertable into alignment post elements that extend from the base plate though the central chamber element and the cover plate. The locking pins include a plurality of locking cams.
摘要翻译： 试样室包括夹在基板和盖板之间的中央室元件，所述基板和盖板将盖板固定在位于中心室部分的与基板和盖板相对的相对表面上的凹陷区域内。 中心室元件包括中心流动通道，其在相对端终止成T形通道。 元件的夹持布置通过锁定销固定，锁销可以插入通过中心室元件和盖板从基板延伸的对准柱元件中。 锁定销包括多个锁定凸轮。
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