公开(公告)号：US20110264380A1

公开(公告)日：2011-10-27

申请号：US13055350

申请日：2009-03-13

申请人： Hervé Cottet ,  Rachid Matmour ,  Jean Philippe Biron ,  Michel Martin

发明人： Hervé Cottet ,  Rachid Matmour ,  Jean Philippe Biron ,  Michel Martin

IPC分类号： G06F19/00 ,  G01N33/00

CPC分类号： B01J19/0053 ,  B01J2208/00955 ,  G01N15/1459 ,  G01N33/442 ,  G01N2015/0092 ,  G01N2030/001

摘要： A method for analysing a mixture M comprising (i) a first monodisperse species, and (ii) a second species having a response coefficient which is distinct from the response coefficient of the first species (i) on at least one detection device, said method comprising the following steps: (A) the mixture M is injected at the inlet of a capillary tube and forced to be transported in said tube by the flow of a carrier liquid induced by a positive hydrodynamic and/or hydrostatic pressure between the inlet and the outlet of the capillary, whereby a phenomenon of Taylor dispersion of the species of the mixture M occurs in the tube; (B) by using a detection device able to detect simultaneously both species (i) and (ii) and placed in the region of the outlet of the capillary tube, a signal reflecting the Taylor dispersion obtained in step (A) is measured; (C) the signal obtained in step (B) is analysed, so as to determine specific contributions of species (i) and (ii) and thereby establishing at least one of the followings: —the content of species (i) and/or (ii) in the mixture M; and/or, —the mean hydrodynamic radius of the species (ii) or the hydrodynamic radius of species (i).

摘要翻译： 一种用于分析混合物M的方法，包括（i）第一单分散物质和（ii）具有与至少一种检测装置上的第一物质（i）的响应系数不同的响应系数的第二物质，所述方法 包括以下步骤：（A）混合物M在毛细管的入口处被注入，并且被迫通过由正的流体动力学和/或静水压力引起的载体流在所述管内被输送到入口和 毛细管的出口，由此在管中发生混合物M的种类的泰勒分散现象; （B）通过使用能够同时检测物质（i）和（ii）并且放置在毛细管的出口的区域中的检测装置，测量反映步骤（A）中获得的泰勒色散的信号; （C）分析步骤（B）中获得的信号，以便确定物种（i）和（ii）的具体贡献，从而建立以下至少一个： - 物种（i）和/或 （ii）在混合物M中; 和/或 - 物种（ii）的平均流体动力学半径或物质（i）的流体动力学半径。

公开(公告)号：US20100300880A1

公开(公告)日：2010-12-02

申请号：US12445660

申请日：2007-10-15

申请人： Hervé Cottet ,  Suzanne Anouti ,  Jean-Philippe Biron ,  Odile Vendenabeele-Trambouze

发明人： Hervé Cottet ,  Suzanne Anouti ,  Jean-Philippe Biron ,  Odile Vendenabeele-Trambouze

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44773

摘要： The present invention relates to a method for separating the components of a mixture M by electrophoresis in a single capillary, wherein: (A) the compounds of the mixture M are introduced and migrate inside the capillary according to the capillary electrophoresis technique; (B) a fraction F of the compounds separated in step (A) is isolated in the capillary and said isolated fraction F is forced to migrate towards the vicinity of one end of the capillary while maintaining this fraction inside the capillary; (C) upon the application of a pressure difference between the ends of the capillary, a second separation medium MS2 different from the first separation medium MS1 of step (A) is introduced into the capillary through the end of the capillary in the vicinity of which the fraction F is located, whereby the fraction F migrates to the other end of the capillary while filling the capillary with the separation medium MS2; and (D) an capillary electrophoresis is carried out in the capillary thus filled with the separation medium MS2, using the medium MS2 as the separation medium.

摘要翻译： 本发明涉及通过在单个毛细管中电泳分离混合物M的组分的方法，其中：（A）根据毛细管电泳技术将混合物M的化合物引入毛细管内迁移; （B）在毛细管中分离步骤（A）中分离的化合物的级分F，并且将所述分离级分F强制迁移到毛细管一端附近，同时保持毛细管内的这一部分; （C）在毛细管端部施加压力差之后，将与步骤（A）的第一分离介质MS1不同的第二分离介质MS2通过其附近的毛细管的末端导入毛细管 定位馏分F，由此馏分F迁移到毛细管的另一端，同时用分离介质MS2填充毛细管; 和（D）使用介质MS2作为分离介质，在毛细管中进行毛细管电泳，从而填充有分离介质MS2。

公开(公告)号：US20060254914A1

公开(公告)日：2006-11-16

申请号：US10569632

申请日：2004-08-23

申请人： Jean-Philippe Biron ,  Hervé Cottet

发明人： Jean-Philippe Biron ,  Hervé Cottet

IPC分类号： C07K1/26

CPC分类号： G01N27/44773

摘要： A method for separating the constituents of a mixture M by electrophoresis in a single capillary includes (A) separating compounds of the mixture M in a single capillary according to the capillary electrophoresis technique; (B) isolating a fraction F of the compounds thus separated by evacuating part of the compounds having the highest migration speeds from the capillary and/or evacuating part of the compounds having the lowest migration speeds from the capillary; (C) introducing a separating medium MS having a higher migration speed than the compounds of the isolated fraction F into the capillary containing the isolated fraction F, and (D) the compounds contained in fraction F are separated in the new electrophoretic conditions thus obtained.

摘要翻译： 通过在单个毛细管中电泳分离混合物M的成分的方法包括：（A）根据毛细管电泳技术在单个毛细管中分离混合物M的化合物; （B）从具有最低迁移速度的化合物的毛细管和/或抽出部分中抽出部分具有最高迁移速率的化合物从毛细管分离出分离的化合物的馏分F; （C）将具有比分离级分F的化合物高的迁移速度的分离介质MS引入含有分离级分F的毛细管中，和（D）将由此获得的新的电泳条件分离出馏分F中所含的化合物。

公开(公告)号：US07837848B2

公开(公告)日：2010-11-23

申请号：US10569632

申请日：2004-08-23

申请人： Jean-Philippe Biron ,  Hervé Cottet

发明人： Jean-Philippe Biron ,  Hervé Cottet

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44773

摘要： A method for separating the constituents of a mixture M by electrophoresis in a single capillary includes (A) separating compounds of the mixture M in a single capillary according to the capillary electrophoresis technique; (B) isolating a fraction F of the compounds thus separated by evacuating part of the compounds having the highest migration speeds from the capillary and/or evacuating part of the compounds having the lowest migration speeds from the capillary; (C) introducing a separating medium MS having a higher migration speed than the compounds of the isolated fraction F into the capillary containing the isolated fraction F, and (D) the compounds contained in fraction F are separated in the new electrophoretic conditions thus obtained.

摘要翻译： 通过在单个毛细管中电泳分离混合物M的成分的方法包括：（A）根据毛细管电泳技术在单个毛细管中分离混合物M的化合物; （B）从具有最低迁移速度的化合物的毛细管和/或抽出部分中抽出部分具有最高迁移速率的化合物从毛细管分离出分离的化合物的馏分F; （C）将具有比分离级分F的化合物高的迁移速度的分离介质MS引入含有分离级分F的毛细管中，和（D）将由此获得的新的电泳条件分离出馏分F中所含的化合物。