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    Insect resistance using inhibition of gene expression
    2.
    发明申请
    Insect resistance using inhibition of gene expression 审中-公开
    昆虫抗性使用抑制基因表达

    公开(公告)号：US20060272049A1

    公开(公告)日：2006-11-30

    申请号：US10579503

    申请日：2004-11-17

    申请人： Peter Waterhouse ,  Steven Whyard ,  Jeroen Van Rie

    发明人： Peter Waterhouse ,  Steven Whyard ,  Jeroen Van Rie

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04 ,  C07K14/415

    CPC分类号： C12N15/8218 ,  C07K14/43563 ,  C12N15/8286 ,  Y02A40/162

    摘要： The current invention provides methods to silence insect genes by using unpackaged dsRNA or siRNA, in one embodiment such dsRNA or siRNA is present in plant vascular tissue, preferably phloem, more particularly phloem sap, and the insect is a plant sap-sucking insect. Also provided are DNA sequences which when transcribed yield a double-stranded RNA molecule capable of reducing the expression of an essential gene of a plant sap-sucking insect, methods of using such DNA sequences and plants or plant cells transformed with such DNA sequences. Also provided is the use of cationic oligopeptides that facilitate the entry of dsRNA or siRNA molecules in insect cells, such as plant sap-sucking insect cells.

    摘要翻译： 本发明提供了通过使用未包装的dsRNA或siRNA使昆虫基因沉默的方法，在一个实施方案中，这种dsRNA或siRNA存在于植物血管组织中，优选韧皮部，尤其是韧皮部液体，昆虫是植物吸吮昆虫。 还提供了当转录时产生能够降低植物吸吮昆虫的必需基因表达的双链RNA分子的DNA序列，使用这种DNA序列的植物或用这种DNA序列转化的植物细胞的方法。 还提供了促进dsRNA或siRNA分子在昆虫细胞中进入的阳离子寡肽的使用，例如植物吸汁昆虫细胞。

    Insecticidal proteins derived from Bacillus Thuringiensis
    6.
    发明申请
    Insecticidal proteins derived from Bacillus Thuringiensis 有权
    衍生自苏云金芽孢杆菌的杀虫蛋白

    公开(公告)号：US20080125366A1

    公开(公告)日：2008-05-29

    申请号：US11892796

    申请日：2007-08-27

    申请人： Greta Arnaut ,  Annemie Boets ,  Karel De Rudder ,  Stijn Vanneste ,  Jeroen Van Rie

    发明人： Greta Arnaut ,  Annemie Boets ,  Karel De Rudder ,  Stijn Vanneste ,  Jeroen Van Rie

    IPC分类号： A61K38/00 ,  A01H5/00 ,  A61P43/00 ,  C07H21/04 ,  C07K14/00 ,  C12N1/20 ,  C12N5/04

    CPC分类号： C07K14/325 ,  A01N63/02 ,  C07K14/32 ,  C12N15/8286

    摘要： The present invention relates to the field of plant pest control, particularly insect control. Provided are nucleotide sequences from Bacillus thuringiensis encoding insecticidal proteins. Further provided are methods and means for using said nucleotide sequence for controlling plant insect pests. It is emphasized that this abstract is provided to comply with the rules requiring an abstract that will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.

    摘要翻译： 本发明涉及植物害虫防治领域，特别是昆虫防治领域。 提供了编码杀虫蛋白的来自苏云金芽孢杆菌的核苷酸序列。 还提供了使用所述核苷酸序列来控制植物害虫的方法和手段。 要强调的是，该摘要被提供以符合要求抽象的规则，允许搜索者或其他读者快速确定技术公开内容的主题。 提交它的理解是，它不会用于解释或限制权利要求的范围或含义。