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    Bacillus thuringiensis strains and their insecticidal proteins
    1.
    发明授权
    Bacillus thuringiensis strains and their insecticidal proteins 失效
    苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号：US07888471B2

    公开(公告)日：2011-02-15

    申请号：US12076204

    申请日：2008-03-14

    申请人： Bart Lambert ,  Stefan Jansens ,  Katrien Van Audenhove ,  Marnix Peferoen

    发明人： Bart Lambert ,  Stefan Jansens ,  Katrien Van Audenhove ,  Marnix Peferoen

    IPC分类号： C07K14/325 ,  C12N15/35

    CPC分类号： C12R1/075 ,  A01N63/00 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译： 四种新型苏云金芽孢杆菌菌株，其保藏在BCCM-LMG，保藏号为。 LMG P-12592，LMG P-12593，LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质，更特别地针对夜蛾科（Spodoptera spp）等等。 和Agrotis ipsilon，针对犀牛科（Ostrinta nubilalis）和针对诸如小菜蛾（Plastella xylostella）的Y科（Yponomeutidae），并由新基因编码。 晶体蛋白含有原毒素，其可以产生作为胰蛋白酶消化产物的毒素。 一种植物，其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化，对鳞翅目具有抗性。 每种菌株本身或其晶体，晶体蛋白质，原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。

    New bacillus thuringiensis strains and their insecticidal proteins
    2.
    发明申请
    New bacillus thuringiensis strains and their insecticidal proteins 失效
    新型苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号：US20090192290A1

    公开(公告)日：2009-07-30

    申请号：US12076204

    申请日：2008-03-14

    申请人： Bart Lambert ,  Stefan Jansens ,  Katrien Van Audenhove ,  Marnix Peferoen

    发明人： Bart Lambert ,  Stefan Jansens ,  Katrien Van Audenhove ,  Marnix Peferoen

    IPC分类号： C07K14/00

    CPC分类号： C12R1/075 ,  A01N63/00 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译： 四种新型苏云金芽孢杆菌菌株，其保藏在BCCM-LMG，保藏号为。 LMG P-12592，LMG P-12593，LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质，更特别地针对夜蛾科（Spodoptera spp）等等。 和Agrotis ipsilon，针对犀牛科（Ostrinta nubilalis）和针对诸如小菜蛾（Plastella xylostella）的Y科（Yponomeutidae），并由新基因编码。 晶体蛋白含有原毒素，其可以产生作为胰蛋白酶消化产物的毒素。 一种植物，其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化，对鳞翅目具有抗性。 每种菌株本身或其晶体，晶体蛋白质，原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。

    Bacillus thuringiensis strains and their insecticidal proteins
    4.
    发明授权
    Bacillus thuringiensis strains and their insecticidal proteins 失效
    苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号：US5885571A

    公开(公告)日：1999-03-23

    申请号：US379656

    申请日：1995-03-23

    申请人： Bart Lambert ,  Stefan Jansens ,  Katrien Van Audenhove ,  Marnix Peferoen

    发明人： Bart Lambert ,  Stefan Jansens ,  Katrien Van Audenhove ,  Marnix Peferoen

    IPC分类号： A01H5/00 ,  A01M1/20 ,  A01N63/00 ,  A01N63/02 ,  C07K14/325 ,  C12N1/20 ,  C12N1/21 ,  C12N5/04 ,  C12N5/10 ,  C12N15/09 ,  C12N15/32 ,  C12N15/82 ,  C12R1/07

    CPC分类号： C12R1/075 ,  A01N63/00 ,  C07K14/325 ,  C12N15/8286

    摘要： Four novel Bacillus thurungienisis strains which are deposited at the BCCM-LMG under accesion nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystals proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译： PCT No.PCT / EP93 / 01820 Sec。 371日期：1995年3月23日 102（e）1995年3月23日PCT PCT 1993年7月12日PCT公布。 公开号WO94 / 05771 日期：1994年3月17日在BCCM-LMG下保存的新型芽孢杆菌属（Thurungienisis）菌株。 LMG P-12592，LMG P-12593，LMG P-12594和LMG P-13493在孢子形成过程中产生对鳞翅目有毒的新的晶体蛋白质，更特别地针对夜蛾科（Spodoptera spp）等等。 和Agrotis ipsilon，针对鞭毛虫如鸵鸟（Ostrinia nubilalis），以及针对pon科（Yponomeutidae）如小菜蛾（Plutella xylostella），并且由新基因编码。 晶体蛋白含有原毒素，其可以产生作为胰蛋白酶消化产物的毒素。 一种植物，其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化，对鳞翅目具有抗性。 每个菌株本身或其晶体，晶体蛋白质，原毒素或毒素都可以用作杀虫鳞虫的杀虫组合物中的活性成分。

    Bacillus thuringiensis insecticidal toxins
    6.
    发明授权
    Bacillus thuringiensis insecticidal toxins 失效
    苏云金芽孢杆菌杀虫毒素

    公开(公告)号：US5683691A

    公开(公告)日：1997-11-04

    申请号：US462319

    申请日：1995-06-05

    申请人： Marnix Peferoen ,  Bart Lambert ,  Henk Joos

    发明人： Marnix Peferoen ,  Bart Lambert ,  Henk Joos

    IPC分类号： C12N15/82 ,  C12N1/20

    CPC分类号： C12N15/8286 ,  Y10S435/832 ,  Y10S530/825

    摘要： Two new Bacillus thuringiensis strains, which are deposited at the DSM under accession nos. 5131 and 5132, produce crystal proteins during sporulation that are toxic to Coleoptera. The crystal proteins contain 74 kDa and 129 kDa protoxins, respectively, which can yield 68 and 66 kDa toxins, respectively, as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that codes for its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Coleoptera.

    摘要翻译： 两种新的苏云金芽孢杆菌菌株，其保藏在帝斯曼登录号。 5131和5132在孢子形成过程中产生对鞘翅目有毒的晶体蛋白质。 晶体蛋白分别含有74kDa和129kDa的原毒素，其分别可产生68和66kDa的毒素作为胰蛋白酶消化产物。 一种植物，其基因组用来自任一种菌株的DNA序列转化，并编码其各自的毒素，对鞘翅目具有抗性。 每种菌株本身或其晶体，晶体蛋白质，原毒素或毒素都可以用作杀虫组合物中的活性成分，用于对抗鞘翅目。

    Bacillus thuringiensis strains and their genes encoding insecticidal toxins
    7.
    发明授权
    Bacillus thuringiensis strains and their genes encoding insecticidal toxins 失效
    苏云金芽孢杆菌菌株及其编码杀虫毒素的基因

    公开(公告)号：US5466597A

    公开(公告)日：1995-11-14

    申请号：US952755

    申请日：1992-11-17

    申请人： Marnix Peferoen ,  Bart Lambert ,  Katrien Van Audenhove

    发明人： Marnix Peferoen ,  Bart Lambert ,  Katrien Van Audenhove

    IPC分类号： A01H1/00 ,  A01H5/00 ,  A01N37/02 ,  A01N63/00 ,  A01N63/02 ,  C07K14/00 ,  C07K14/195 ,  C07K14/325 ,  C07K14/41 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/32 ,  C12N15/82 ,  C12P21/02 ,  C12P21/06 ,  C12R1/07 ,  C12R1/19 ,  C07H17/00

    CPC分类号： C12R1/075 ,  A01N63/00 ,  C07K14/325 ,  C12N15/8286 ,  Y10S435/832

    摘要： Two new Bacillus thuringiensis strains, which are deposited at the DSM under accession nos 5870 and 5871, produce new crystal proteins during sporulation that are toxic to Coleoptera and that are encoded by new genes. The crystal proteins contain protoxins, which can yield toxins as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes an insecticidally effective portion of its respective protoxin or encodes its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin, toxin and/or insecticidally effective protoxin portion can be used as the active ingredient in an insecticidal composition for combating Coleoptera.

    摘要翻译： PCT No.PCT / EP91 / 00791 Sec。 371日期：1992年11月17日 102（e）日期：1992年11月17日PCT 1991年4月24日PCT PCT。 出版物WO91 / 16433 1991年10月31日。两种新型苏云金芽孢杆菌菌株在保藏号为5870和5871的DSM下保藏，在孢子形成过程中产生新的晶体蛋白，对鞘翅目有毒，并由新基因编码。 晶体蛋白含有原毒素，其可产生作为胰蛋白酶消化产物的毒素。 一种植物，其基因组用来自任一种菌株的DNA序列转化并编码其各自原毒素的杀虫有效部分或编码其各自的毒素，对鞘翅目具有抗性。 每种菌株本身或其晶体，晶体蛋白质，原毒素，毒素和/或杀虫有效的前毒素部分可以用作用于防治鞘翅目的杀虫组合物中的活性成分。

    Bacillus thuringiensis strains and their insecticidal proteins
    9.
    发明授权
    Bacillus thuringiensis strains and their insecticidal proteins 失效
    苏云金芽孢杆菌菌株及其杀虫蛋白

    公开(公告)号：US07361751B2

    公开(公告)日：2008-04-22

    申请号：US10687879

    申请日：2003-10-20

    申请人： Bart Lambert ,  Stefan Jansens ,  Katrien Van Audenhove ,  Marnix Peferoen

    发明人： Bart Lambert ,  Stefan Jansens ,  Katrien Van Audenhove ,  Marnix Peferoen

    IPC分类号： C12N15/32 ,  C07K14/325

    CPC分类号： C12R1/075 ,  A01N63/00 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

    摘要： Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as Spodoptera spp. and Agrotis ipsilon, against Pyralidae such as Ostrinta nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that encodes its respective toxin, is resistant to Lepidoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Lepidoptera.

    摘要翻译： 四种新型苏云金芽孢杆菌菌株，其保藏在BCCM-LMG，保藏号为。 LMG P-12592，LMG P-12593，LMG P-12594和LMG P-13493在孢子形成期间产生对鳞翅目有毒的新的晶体蛋白质，更特别地针对夜蛾科（Spodoptera spp）等等。 和Agrotis ipsilon，针对犀牛科（Ostrinta nubilalis）和针对诸如小菜蛾（Plastella xylostella）的Y科（Yponomeutidae），并由新基因编码。 晶体蛋白含有原毒素，其可以产生作为胰蛋白酶消化产物的毒素。 一种植物，其基因组用来自任一种菌株并编码其各自毒素的DNA序列转化，对鳞翅目具有抗性。 每种菌株本身或其晶体，晶体蛋白质，原毒素或毒素都可用作杀虫鳞虫的杀虫组合物中的活性成分。