公开(公告)号：US20140349356A1

公开(公告)日：2014-11-27

申请号：US14344608

申请日：2012-09-13

申请人： Min Jiang ,  Min Zhang ,  Jiangfeng Ma ,  Hao Wu ,  Liya Liang ,  Rongming Liu ,  Guangming Wang ,  Ping Wei

发明人： Min Jiang ,  Min Zhang ,  Jiangfeng Ma ,  Hao Wu ,  Liya Liang ,  Rongming Liu ,  Guangming Wang ,  Ping Wei

IPC分类号： C12P7/46

CPC分类号： C12P7/46 ,  C07C51/412 ,  C07C51/43 ,  C07C51/44 ,  C07C55/10

摘要： This invention belongs to the field of biochemical engineering and relates to a method of cyclic utilization of water during separation of succinic acid made by fermentation. This invention uses water from separation process for aerobic growth of E.coli AFP111 and production of succinic acid by anaerobic fermentation, obtaining final succinic acid concentration of 55 g/L and yield of 91.6%. Compared with results of fermentation using culture medium prepared from tap water, succinic acid concentration and productivity increased by 8.5% and 8.46%, respectively. An outstanding advantage of this invention is recovery and utilization of evaporated water during separation of succinic acid, realizing cyclic use of water during industrial production of succinic acid, which is an environment-friendly process. Also, as evaporated water generated during separation of succinic acid contains small amount of organic acids such as acetic acid and formic acid, if this water is used for aerobic growth of thalli, the small amount of organic acids contained therein can be used as gluconeogenesis carbon source, improving activity of some key enzymes in cell and favoring succinic acid production by anaerobic fermentation of thalli.

摘要翻译： 本发明属于生物化学工程领域，涉及通过发酵制备的琥珀酸分离期间循环利用水的方法。 本发明利用分离过程中的水分进行大肠杆菌AFP111的有氧生长，通过厌氧发酵生产琥珀酸，得到最终琥珀酸浓度为55g / L，产率为91.6％。 与使用自来水制备的培养基的发酵结果相比，琥珀酸浓度和产率分别提高了8.5％和8.46％。 本发明的突出优点是分离琥珀酸期间蒸发水的回收利用，在工业生产琥珀酸期间循环使用水，这是一个环保的工艺。 另外，由于琥珀酸分离过程中产生的蒸发水含有少量的有机酸如乙酸和甲酸，所以如果这种水用于铊的有氧生长，其中所含的少量有机酸可用作糖异生碳 来源，改善细胞中一些关键酶的活性，并通过thalli的厌氧发酵有利于琥珀酸生产。

公开(公告)号：US20140155674A1

公开(公告)日：2014-06-05

申请号：US14234807

申请日：2012-07-16

申请人： Dehua Wang ,  Zhuo Yu ,  Huiguo Wang ,  Jiangfeng Ma

发明人： Dehua Wang ,  Zhuo Yu ,  Huiguo Wang ,  Jiangfeng Ma

IPC分类号： C07C7/12 ,  B01D15/02

CPC分类号： C07C7/12 ,  B01D15/02 ,  B01D15/1835 ,  C07C7/13 ,  C07C15/08

摘要： The present invention relates to a process for separating isomers by simulated moving bed (SMB) adsorption, comprising separating the raw materials comprising isomers by SMB adsorption, said SMB comprising many adsorption beds each of which is equipped with grids, each of the grids being equipped with the feedstock inlet and outlet pipeline of the bed, the feedstock charged into and discharged from SMB at least comprising feedstocks, desorbent, extract, and raffinate, the extract being enriched with the target product, characterized in that the extract is used as a flushing liquid and respectively charged to first or second bed at the upstream of the feedstock charging position and to one of second to fourth beds at the downstream of the extract withdrawing position. Such process is used for separation of C8 aromatic isomers by adsorption, and can improve the capacity of the device while effectively increasing the purity of the target product separated by adsorption.

摘要翻译： 本发明涉及通过模拟移动床（SMB）吸附分离异构体的方法，包括通过SMB吸附分离包含异构体的原料，所述SMB包括多个吸附床，每个吸附床配备有格栅，每个网格装备有 与床的原料入口和出口管线相连，原料至少包含原料，解吸剂，提取物和萃余液，并从SMB中排出，该提取物富含目标产物，其特征在于该提取物用作冲洗 液体并分别装入在原料装料位置的上游的第一床或第二床，并在提取物抽出位置的下游分别装入第二至第四床中的一个。 这种方法用于通过吸附分离C8芳族异构体，并且可以提高装置的容量，同时有效提高通过吸附分离的目标产物的纯度。

公开(公告)号：US09562242B2

公开(公告)日：2017-02-07

申请号：US14344608

申请日：2012-09-13

申请人： Min Jiang ,  Min Zhang ,  Jiangfeng Ma ,  Hao Wu ,  Liya Liang ,  Rongming Liu ,  Guangming Wang ,  Ping Wei

发明人： Min Jiang ,  Min Zhang ,  Jiangfeng Ma ,  Hao Wu ,  Liya Liang ,  Rongming Liu ,  Guangming Wang ,  Ping Wei

IPC分类号： C12P7/46 ,  C07C51/41 ,  C07C51/43 ,  C07C51/44

CPC分类号： C12P7/46 ,  C07C51/412 ,  C07C51/43 ,  C07C51/44 ,  C07C55/10

摘要： This invention belongs to the field of biochemical engineering and relates to a method of cyclic utilization of water during separation of succinic acid made by fermentation. This invention uses water from separation process for aerobic growth of E. coli AFP111 and production of succinic acid by anaerobic fermentation, obtaining final succinic acid concentration of 55 g/L and yield of 91.6%. Compared with results of fermentation using culture medium prepared from tap water, succinic acid concentration and productivity increased by 8.5% and 8.46%, respectively. An outstanding advantage of this invention is recovery and utilization of evaporated water during separation of succinic acid, realizing cyclic use of water during industrial production of succinic acid, which is an environment-friendly process. Also, as evaporated water generated during separation of succinic acid contains small amount of organic acids such as acetic acid and formic acid, if this water is used for aerobic growth of thalli, the small amount of organic acids contained therein can be used as gluconeogenesis carbon source, improving activity of some key enzymes in cell and favoring succinic acid production by anaerobic fermentation of thalli.

摘要翻译： 本发明属于生物化学工程领域，涉及通过发酵制备的琥珀酸分离期间循环利用水的方法。 本发明利用分离过程中的水分进行大肠杆菌AFP111的有氧生长，通过厌氧发酵生产琥珀酸，得到最终琥珀酸浓度为55g / L，产率为91.6％。 与使用自来水制备的培养基的发酵结果相比，琥珀酸浓度和产率分别提高了8.5％和8.46％。 本发明的突出优点是分离琥珀酸期间蒸发水的回收利用，在工业生产琥珀酸期间循环使用水，这是一个环保的工艺。 另外，由于琥珀酸分离过程中产生的蒸发水含有少量的有机酸如乙酸和甲酸，所以如果这种水用于铊的有氧生长，其中所含的少量有机酸可用作糖异生碳 来源，改善细胞中一些关键酶的活性，并通过thalli的厌氧发酵有利于琥珀酸生产。