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    Use of Bacterial Beta-Lactamase for In Vitro Diagnostics and In Vivo Imaging, Diagnostics and Therapeutics
    1.
    发明申请
    Use of Bacterial Beta-Lactamase for In Vitro Diagnostics and In Vivo Imaging, Diagnostics and Therapeutics 审中-公开
    使用细菌β-内酰胺酶进行体外诊断和体内成像，诊断和治疗

    公开(公告)号：US20160376629A1

    公开(公告)日：2016-12-29

    申请号：US15262374

    申请日：2016-09-12

    Applicant: Jeffrey D. Cirillo ,  Jianghong Rao

    Inventor： Jeffrey D. Cirillo ,  Jianghong Rao

    IPC: C12Q1/18 ,  G01N21/64 ,  G01N21/78

    CPC classification number: C12Q1/18 ,  A61K49/0013 ,  A61K49/0021 ,  A61K49/0028 ,  A61K49/0032 ,  A61K49/0041 ,  A61K49/0052 ,  C09B57/02 ,  C12Q1/04 ,  C12Q1/34 ,  C12Q1/66 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/78 ,  G01N2021/6417 ,  G01N2201/061 ,  G01N2333/986

    Abstract: Provided herein provided is an assay system for monitoring drug susceptibility of a pathogenic bacteria comprising color-producing substrates for a beta-lactamase of the pathogenic bacteria, an assay device for visibly detecting a product of the beta-lactamase on the substrate thereof and a reader configured to quantify the visibly detected product. Also provided is an in vitro method to determine susceptibility to a drug effective against a pathogenic bacteria, for example, a pathogenic Mycobacteria, that has a beta-lactamase activity. An excitation wavelength is delivered to a biological sample obtained from a subject having an infection from the pathogenic bacteria in the presence of a beta-lactamase substrate. The intensity of a signal, such as a fluorescent, luminescent or colorimetric signal, at an emission wavelength of a product of the beta-lactamase on the subject is correlated to drug susceptibility.

    Abstract translation: 本文提供了一种用于监测病原细菌的药物敏感性的测定系统，其包括致病细菌的β-内酰胺酶的着色产生底物，用于可见地检测其底物上的β-内酰胺酶的产物和读数器 配置为量化可见检测到的产品。 还提供了一种体外方法来确定对具有β-内酰胺酶活性的致病细菌例如致病分枝杆菌有效的药物的易感性。 在β-内酰胺酶底物的存在下，将激发波长输送到从具有病原菌感染的受试者获得的生物样品。 在受试者的β-内酰胺酶的产物的发射波长处的信号强度，例如荧光，发光或比色信号与药物敏感性相关。

    Hydrolase detection system with caged substrates
    3.
    发明授权
    Hydrolase detection system with caged substrates 失效
    水解酶检测系统与笼式底物

    公开(公告)号：US08530178B2

    公开(公告)日：2013-09-10

    申请号：US12445378

    申请日：2007-10-17

    Applicant: Daniel Sobek ,  Jianghong Rao

    Inventor： Daniel Sobek ,  Jianghong Rao

    IPC: C12Q1/42

    CPC classification number: C12Q1/34

    Abstract: An enzyme detection method includes forming a caged substrate; releasing an uncaged substrate by cleaving a caging molecule from the caged substrate; and emitting a light emission from a Bioluminescence Resonance Energy Transfer luminescent nanocrystal conjugate reacting with the uncaged substrate.

    Abstract translation: 酶检测方法包括形成笼状基材; 通过从笼形基底切割笼状分子释放未起皮的底物; 并且从与未起始的底物反应的生物发光共振能量转移发光纳米晶体共轭物发射发光。

    ACTIVATABLE BIOLUMINESCENT PROBE SYSTEM AND METHOD OF USE THEREOF
    6.
    发明申请
    ACTIVATABLE BIOLUMINESCENT PROBE SYSTEM AND METHOD OF USE THEREOF 审中-公开
    可激活的生物发光探测系统及其使用方法

    公开(公告)号：US20110200529A1

    公开(公告)日：2011-08-18

    申请号：US13119721

    申请日：2009-09-17

    Applicant: Jianghong Rao ,  Zuyong Xia

    Inventor： Jianghong Rao ,  Zuyong Xia

    IPC: A61K49/00 ,  G01N21/76 ,  C12M1/34

    CPC classification number: G01N33/542

    Abstract: A method of use of an activatable bioluminescent probe system includes: providing a bioluminescent protein and a quencher in a reaction environment; modifying a ligand between the quencher and the bioluminescent protein using a ligand interacting molecule; adding a bioluminescence initiating molecule to the reaction environment; and measuring light originating from the interaction between the bioluminescent protein and the bioluminescence initiating molecule.

    Abstract translation: 使用可激活的生物发光探针系统的方法包括：在反应环境中提供生物发光蛋白和猝灭剂; 使用配体相互作用分子修饰猝灭剂和生物发光蛋白质之间的配体; 在反应环境中加入生物发光引发分子; 并测量源自生物发光蛋白和生物发光起始分子之间相互作用的光。

    Bioluminogenic assay system for measuring beta-lactamase activity
    7.
    发明授权
    Bioluminogenic assay system for measuring beta-lactamase activity 有权
    用于测量β-内酰胺酶活性的生物发光测定系统

    公开(公告)号：US07867758B2

    公开(公告)日：2011-01-11

    申请号：US12060864

    申请日：2008-04-01

    Applicant: Jianghong Rao ,  Min-kyung So ,  Hequan Yao

    Inventor： Jianghong Rao ,  Min-kyung So ,  Hequan Yao

    IPC: C12M1/00 ,  C12Q1/34

    CPC classification number: C12Q1/66 ,  C12Q1/34 ,  G01N2333/986

    Abstract: A bioluminogenic assay system including: providing a bioluminogenic substrate incorporating a beta-lactam antibiotic, a bioluminescence initiating compound, and a chemical linkage joining the beta-lactam antibiotic to the bioluminescence initiating compound; exposing the bioluminogenic substrate to a beta-lactamase enzyme that catalyzes the release of the bioluminescence initiating compound from the bioluminogenic substrate; co-exposing the bioluminogenic substrate to a bioluminescence indicator reaction that employs the bioluminescence initiating compound as a substrate; and detecting a light from the bioluminescence indicator reaction as a measure of the activity of the beta-lactamase enzyme.

    Abstract translation: 生物发光测定系统，包括：提供掺入β-内酰胺抗生素的生物发光底物，生物发光引发化合物和将β-内酰胺抗生素与生物发光引发化合物连接的化学键; 将生物发光底物暴露于催化生物发光起始化合物从生物发光底物释放的β-内酰胺酶; 将生物发光底物共同暴露于采用生物发光引发化合物作为底物的生物发光指示剂反应; 并检测来自生物发光指示剂反应的光作为β-内酰胺酶活性的量度。

    Beta-lactamase substrates having phenolic ethers
    10.
    发明授权
    Beta-lactamase substrates having phenolic ethers 有权
    具有酚醚的β-内酰胺酶底物

    公开(公告)号：US07396926B2

    公开(公告)日：2008-07-08

    申请号：US10044486

    申请日：2002-01-11

    Applicant: Roger Y. Tsien ,  Jianghong Rao

    Inventor： Roger Y. Tsien ,  Jianghong Rao

    IPC: C07D501/34 ,  C07D501/30 ,  C07D501/28 ,  C07D501/24 ,  C07D505/18 ,  C07D505/20 ,  C07D519/06 ,  G01N33/573 ,  C07D463/22 ,  C07D463/20 ,  C07D463/18

    CPC classification number: C12Q1/6897 ,  C07D463/18 ,  C07D501/00 ,  C07D505/00 ,  C07F9/65613 ,  C12Q1/34

    Abstract: Provided are fluorescent substrates for β-lactamases having the general formula I: in which R is a benzyl, 2-thienylmethyl, or cyanomethyl group; R′ is selected from the group consisting of H, physiologically acceptable salts or metal, ester groups, ammonium cations, —CHR2OCO(CH2)nCH3, —CHR2OCOC(CH3)3, acylthiomethyl, acyloxy-alpha-benzyl, deltabutyrolactonyl, methoxycarbonyloxymethyl, phenyl, methylsulphinylmethyl, β-morpholinoethyl, dialkylaminoethyl, and dialkylaminocarbonyloxymethyl, in which R2 is selected from the group consisting of H and lower alkyl; A is selected from the group consisting of S, O, SO, SO2 and CH2; and Z is a donor fluorescent moiety. Also provided are methods of use of the compound of general formula I.

    Abstract translation: 提供具有通式I的β-内酰胺酶的荧光底物：其中R是苄基，2-噻吩基甲基或氰基甲基; R'选自H，生理上可接受的盐或金属，酯基，铵阳离子，-CHR 2 OCO（CH 2）n， （CH 3）3，酰基硫甲基，酰氧基-α- 苄基，异丙基丁内酰胺，甲氧基羰氧基甲基，苯基，甲基亚磺酰基甲基，β-吗啉代乙基，二烷基氨基乙基和二烷基氨基羰基氧基甲基，其中R 2选自H和低级烷基; A选自S，O，SO，SO 2和CH 2; Z是供体荧光部分。 还提供了通式I化合物的使用方法。

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