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公开(公告)号：US20140227709A1
公开(公告)日：2014-08-14
申请号：US14252755
申请日：2014-04-14
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L7/52 , B01L2200/10 , B01L2200/16 , B01L2300/1827 , B01L2400/0406 , C12Q1/6837 , C12Q1/6851 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143 , C12Q2547/107 , C12Q2537/157
摘要： Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.
摘要翻译： 提供了用于检测样品中感兴趣的低拷贝核酸的方法。 在一种方法中，将包含感兴趣的核酸的样品等分成多个反应混合物，其中至少两个是单拷贝反应混合物。 反应混合物在流过微流体装置的通道时进行一次或多次扩增反应。 将至少一种反应混合物配制在包含悬浮在不混溶液体中的水性液滴的乳液的水相中。 感兴趣的核酸在进行扩增反应之前以水相的至少一个水滴形式存在于单拷贝中。 当将其配制在乳液中时，对反应混合物进行扩增。 在该方法的多个步骤期间，核酸连续流动。
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2.发明申请公开(公告)号：US20100129896A1
公开(公告)日：2010-05-27
申请号：US12684601
申请日：2010-01-08
IPC分类号： C12M1/00
CPC分类号： C12Q1/686 , B01L3/5027 , B01L7/52 , B01L2200/10 , B01L2200/16 , B01L2300/1827 , B01L2400/0406 , C12Q1/6837 , C12Q1/6851 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143 , C12Q2547/107 , C12Q2537/157
摘要： Systems for differentiating the lengths of nucleic acids of interest in a sample are provided. The system includes a microfluidic device, a detector, and a software system. The microfluidic device includes an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acid of interest. The detector is integral with or proximal to the microfluidic device and is configured to detect the amplicons as one or more signals from a homogenous mixture. The software system interprets one or more coincidentally detected signals to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.
摘要翻译： 提供了用于区分样品中感兴趣的核酸长度的系统。 该系统包括微流体装置，检测器和软件系统。 微流体装置包括在提供感兴趣的核酸的一个或多个扩增子的条件下含有反应混合物的扩增微通道或微室。 检测器与微流体装置成一体或靠近微流体装置，并且被配置为将扩增子检测为来自均匀混合物的一个或多个信号。 软件系统解释一个或多个巧合检测的信号以指示感兴趣的一个或多个单个核酸分子的长度，从而区分感兴趣的核酸的长度。
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公开(公告)号：US06881312B2
公开(公告)日：2005-04-19
申请号：US10319045
申请日：2002-12-13
申请人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
发明人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
IPC分类号： B01L3/00 , B01L7/00 , B01L9/00 , G01N21/05 , G01N21/64 , G01N27/447 , G01N35/00 , G01N37/00 , G01N27/26
CPC分类号： G01N21/6452 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00628 , B01J2219/0063 , B01J2219/00659 , B01J2219/00707 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/54 , B01L9/527 , B01L2200/023 , B01L2200/027 , B01L2200/028 , B01L2200/147 , B01L2300/0654 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N27/44721 , G01N27/44782 , G01N35/0099 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421
摘要： Analytical systems and methods that use a modular interface structure for providing an interface between a sample substrate and an analytical unit, where the analytical unit typically has a particular interface arrangement for implementing various analytical and control functions. Using a number of variants for each module of the modular interface structure advantageously provides cost effective and efficient ways to perform numerous tests using a particular substrate or class of substrates with a particular analytical and control systems interface arrangement. Improved optical illumination and detection system for simultaneously analyzing reactions or conditions in multiple parallel microchannels are also provided. Increased throughput and improved emissions detection is provided by the present invention by simultaneously illuminating multiple parallel microchannels at a non-normal incidence using an excitation beam including multiple excitation frequencies, and simultaneously detecting emissions from the substances in the microchannels in a direction normal to the substrate using a detection module with multiple detectors.
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公开(公告)号：US06681616B2
公开(公告)日：2004-01-27
申请号：US10008604
申请日：2001-11-09
申请人： Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
发明人： Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
IPC分类号： G01N1106
CPC分类号： G05D7/0694 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L9/527 , B01L2200/027 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N11/08 , G01N27/44791 , G01N2035/1044 , Y10T137/8158
摘要： Microfluidic devices, systems, and methods measure viscosity, flow times, and/or other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.
摘要翻译： 微流体装置，系统和方法测量通道内的粘度，流动时间和/或其他流动特性，并且可以使用测量的流动特性来产生期望的流量。 多储层压力调节器和压力控制器系统，电动系统和/或其它流体输送机构可以产生流动，可控地混合流体等。
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公开(公告)号：US06990851B2
公开(公告)日：2006-01-31
申请号：US10728528
申请日：2003-12-05
申请人： Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
发明人： Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
CPC分类号： G05D7/0694 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L9/527 , B01L2200/027 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N11/08 , G01N27/44791 , G01N2035/1044 , Y10T137/8158
摘要： Microfluidic devices, systems, and methods measure viscosity, flow times, and/or other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.
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公开(公告)号：US07040144B2
公开(公告)日：2006-05-09
申请号：US10207735
申请日：2002-07-26
申请人： Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill , Josh Molho , Anubhav Tripathi , Matthew B. Kerby
发明人： Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill , Josh Molho , Anubhav Tripathi , Matthew B. Kerby
CPC分类号： G01N27/44791 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L9/527 , B01L2200/027 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N11/08 , G01N2035/1044 , G05D7/0694
摘要： Microfluidic devices, systems, and methods measure viscosity, flow times, and/or pressures, other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.
摘要翻译： 微流体装置，系统和方法测量通道内的粘度，流动时间和/或压力，其它流动特性，并且所测量的流动特性可用于产生所需的流量。 多储层压力调节器和压力控制器系统，电动系统和/或其它流体输送机构可以产生流动，可控地混合流体等。
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公开(公告)号：US06547941B2
公开(公告)日：2003-04-15
申请号：US09920090
申请日：2001-07-31
申请人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
发明人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
IPC分类号： G01N2726
CPC分类号： G01N21/6452 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00628 , B01J2219/0063 , B01J2219/00659 , B01J2219/00707 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/54 , B01L9/527 , B01L2200/023 , B01L2200/027 , B01L2200/028 , B01L2200/147 , B01L2300/0654 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N27/44721 , G01N27/44782 , G01N35/0099 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421
摘要： Analytical systems and methods that use a modular interface structure for providing an interface between a sample substrate and an analytical unit, where the analytical unit typically has a particular interface arrangement for implementing various analytical and control functions. Using a number of variants for each module of the modular interface structure advantageously provides cost effective and efficient ways to perform numerous tests using a particular substrate or class of substrates with a particular analytical and control systems interface arrangement. Improved optical illumination and detection system for simultaneously analyzing reactions or conditions in multiple parallel microchannels are also provided. Increased throughput and improved emissions detection is provided by the present invention by simultaneously illuminating multiple parallel microchannels at a non-normal incidence using an excitation beam including multiple excitation frequencies, and simultaneously detecting emissions from the substances in the microchannels in a direction normal to the substrate using a detection module with multiple detectors.
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公开(公告)号：US06358387B1
公开(公告)日：2002-03-19
申请号：US09536274
申请日：2000-03-27
申请人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
发明人： Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
IPC分类号： C02F140
CPC分类号： G01N21/6452 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00628 , B01J2219/0063 , B01J2219/00659 , B01J2219/00707 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/54 , B01L9/527 , B01L2200/023 , B01L2200/027 , B01L2200/028 , B01L2200/147 , B01L2300/0654 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N27/44721 , G01N27/44782 , G01N35/0099 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421
摘要： Analytical systems and methods that use a modular interface structure for providing an interface between a sample substrate and an analytical unit, where the analytical unit typically has a particular interface arrangement for implementing various analytical and control functions. Using a number of variants for each module of the modular interface structure advantageously provides cost effective and efficient ways to perform numerous tests using a particular substrate or class of substrates with a particular analytical and control systems interface arrangement. Improved optical illumination and detection system for simultaneously analyzing reactions or conditions in multiple parallel microchannels are also provided. Increased throughput and improved emissions detection is provided by the present invention by simultaneously illuminating multiple parallel microchannels at a non-normal incidence using an excitation beam including multiple excitation frequencies, and simultaneously detecting emissions from the substances in the microchannels in a direction normal to the substrate using a detection module with multiple detectors.
摘要翻译： 分析系统和方法使用模块化接口结构来提供样品基底和分析单元之间的界面，其中分析单元通常具有用于实现各种分析和控制功能的特定界面布置。 使用模块化接口结构的每个模块的许多变体有利地提供了使用具有特定分析和控制系统接口布置的特定基板或类别的基板执行大量测试的成本有效和有效的方式。 还提供了用于同时分析多个并行微通道中的反应或条件的改进的光学照明和检测系统。 通过使用包括多个激发频率的激发束同时照射非法线入射的多个平行微通道并且同时在垂直于衬底的方向上检测来自微通道中的物质的发射，本发明提供了增加的吞吐量和改进的排放物检测 使用具有多个检测器的检测模块。
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公开(公告)号：US07695981B2
公开(公告)日：2010-04-13
申请号：US11433824
申请日：2006-05-15
申请人： Haixia Dai , Khashayar Pakbaz , Michael Spaid , Theo Nikiforov
发明人： Haixia Dai , Khashayar Pakbaz , Michael Spaid , Theo Nikiforov
IPC分类号： H01L21/00
CPC分类号： C23C18/1608 , B82Y30/00 , C23C18/1844 , C23C18/50 , H01L21/288 , H01L21/76814 , H01L21/76843 , H01L21/76849 , H01L21/7685 , H01L21/76861 , H01L21/76864 , H01L21/76867 , H01L21/76868 , H01L21/76871 , H01L21/76874 , H01L21/76879
摘要： A seed layer is formed on a substrate using a first biological agent. The seed layer may comprise densified nanoparticles which are bound to the biological agent. The seed layer is then used for a deposition of a metal layer, such as a barrier layer, an interconnect layer, a cap layer and/or a bus line for a solid state device.
摘要翻译： 使用第一生物试剂在基材上形成种子层。 种子层可以包含与生物制剂结合的致密化纳米颗粒。 然后种子层用于沉积用于固态器件的金属层，例如阻挡层，互连层，盖层和/或总线。
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公开(公告)号：US20070261479A1
公开(公告)日：2007-11-15
申请号：US11750950
申请日：2007-05-18
申请人： Michael Spaid , Jeffrey Wolk
发明人： Michael Spaid , Jeffrey Wolk
IPC分类号： G01N33/00
CPC分类号： G01N33/558
摘要： The present invention relates to novel methods and systems for determining the interaction of molecules using the phenomenon of Taylor-Aris dispersion present in fluid flow in conduits. The method involves relating a change in dispersion of molecules to their level of interaction. The present invention also relates to an assay method using Taylor-Aris dispersion in a microfluidic system in order to examine molecular interactions in a variety of chemical and biochemical systems.
摘要翻译： 本发明涉及使用存在于管道中的流体流中的泰勒 - 阿里斯分散现象来确定分子相互作用的新方法和系统。 该方法涉及将分子的分散变化与其相互作用的水平相关联。 本发明还涉及在微流体系统中使用泰勒 - 阿里斯分散体以测试各种化学和生化系统中的分子相互作用的测定方法。
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