公开(公告)号：US08114617B2

公开(公告)日：2012-02-14

申请号：US12970250

申请日：2010-12-16

申请人： Kevin J. Reagan ,  Erik Schaefer ,  Jimin Wang

发明人： Kevin J. Reagan ,  Erik Schaefer ,  Jimin Wang

IPC分类号： G01N33/53 ,  G01N33/543

CPC分类号： C07K16/2863 ,  C07K16/18 ,  C07K2317/34 ,  C12Q1/485 ,  G01N2333/4709

摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.

摘要翻译： 本发明涉及一种用于测量特定位点蛋白质磷酸化的方法，因此是能够使这些位点磷酸化的酶的蛋白激酶活性的指标。 该方法包括在特定丝氨酸，苏氨酸或酪氨酸残基处的靶蛋白的体外或体内磷酸化，使该蛋白质（非磷酸化）到含有所有试剂的反应混合物，包括磷酸激酶，其允许产生磷酸化形式 蛋白。 通过使磷酸化蛋白质与针对磷酸化位点特异性的抗体接触来测量磷酸化蛋白质。 本发明包括可用于实践本发明方法的抗体。 本发明特别涉及通过磷酸化和去磷酸化修饰的所有蛋白质，如Tau，Rb和EGFR蛋白质所示，以及对Tau，Rb或EGFR蛋白质的磷酸化位点特异的抗体。

公开(公告)号：US09130142B2

公开(公告)日：2015-09-08

申请号：US14111683

申请日：2012-04-11

申请人： Xiaozhong Zhang ,  Caihua Wan ,  Xili Gao ,  Jimin Wang ,  Lihua Wu

发明人： Xiaozhong Zhang ,  Caihua Wan ,  Xili Gao ,  Jimin Wang ,  Lihua Wu

IPC分类号： H01L29/82 ,  H01L43/02 ,  H01L43/08 ,  H01L43/12

CPC分类号： H01L43/02 ,  H01L43/08 ,  H01L43/12

摘要： The present invention relates to a magnetoresistance device using a semiconductor substrate and a method for manufacturing the same. The magnetoresistance device includes: a semiconductor substrate; an oxidation layer disposed on a surface of the semiconductor substrate; electrodes disposed on the oxidation layer; and at least one diode connected between at least two of the electrodes. The magnetoresistance device of the present invention has excellent performances of a high field magnetoresistance characteristic and high sensitivity at low magnetic field, and has advantages of low power consumption, simple device structure, low cost and simple manufacturing process.

摘要翻译： 本发明涉及使用半导体衬底的磁阻器件及其制造方法。 磁阻器件包括：半导体衬底; 设置在所述半导体衬底的表面上的氧化层; 设置在氧化层上的电极; 以及连接在至少两个电极之间的至少一个二极管。 本发明的磁电阻器件在低磁场下具有优异的高场磁阻特性和高灵敏度的性能，具有功耗低，器件结构简单，成本低，制造工艺简单等优点。

公开(公告)号：US06631329B1

公开(公告)日：2003-10-07

申请号：US09629732

申请日：2000-07-31

申请人： Jimin Wang ,  Laura F. Silvian ,  Thomas A. Steitz

发明人： Jimin Wang ,  Laura F. Silvian ,  Thomas A. Steitz

IPC分类号： G06F1900

CPC分类号： C12N9/93

摘要： The present invention provides the atomic coordinates derived from high resolution x-ray diffraction of the cocrystal complex comprising mupirocin with its target enzyme, isoleucyl-tRNA synthetase from Staphylococcus aureus, and the cognate tRNAile from Escherichia coli. The present invention further provides methods of using the atomic coordinates to identify and design new agents which modulate protein synthesis as well as the agents themselves.

摘要翻译： 本发明提供衍生自包含莫匹罗星与其目标酶的共晶体络合物的高分辨率x射线衍射的原子坐标，来自金黄色葡萄球菌的异亮氨酰-tRNA合成酶和来自大肠杆菌的同源tRNA。 本发明还提供使用原子坐标来鉴定和设计调节蛋白质合成的新试剂以及试剂本身的方法。

公开(公告)号：US20090104628A1

公开(公告)日：2009-04-23

申请号：US12179526

申请日：2008-07-24

申请人： Kevin J. REAGAN ,  Erik Schaefer ,  Jimin Wang

发明人： Kevin J. REAGAN ,  Erik Schaefer ,  Jimin Wang

IPC分类号： G01N33/573 ,  C07K16/40

CPC分类号： C07K16/2863 ,  C07K16/18 ,  C07K2317/34 ,  C12Q1/485 ,  G01N2333/4709

摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.

摘要翻译： 本发明涉及一种用于测量特定位点蛋白质磷酸化的方法，因此是能够使这些位点磷酸化的酶的蛋白激酶活性的指标。 该方法包括在特定丝氨酸，苏氨酸或酪氨酸残基处的靶蛋白的体外或体内磷酸化，使该蛋白质（非磷酸化）到含有所有试剂的反应混合物，包括磷酸激酶，其允许产生磷酸化形式 蛋白。 通过使磷酸化蛋白质与针对磷酸化位点特异的抗体接触来测量磷酸化蛋白质。 本发明包括可用于实践本发明方法的抗体。 本发明特别涉及通过磷酸化和去磷酸化修饰的所有蛋白质，如Tau，Rb和EGFR蛋白质所示，以及对Tau，Rb或EGFR蛋白质的磷酸化位点特异的抗体。

公开(公告)号：US20140035076A1

公开(公告)日：2014-02-06

申请号：US14111683

申请日：2012-04-11

申请人： Xiaozhong Zhang ,  Caihua Wan ,  Xili Gao ,  Jimin Wang ,  Lihua Wu

发明人： Xiaozhong Zhang ,  Caihua Wan ,  Xili Gao ,  Jimin Wang ,  Lihua Wu

IPC分类号： H01L43/02 ,  H01L43/12

CPC分类号： H01L43/02 ,  H01L43/08 ,  H01L43/12

摘要： The present invention relates to a magnetoresistance device using a semiconductor substrate and a method for manufacturing the same. The magnetoresistance device includes: a semiconductor substrate; an oxidation layer disposed on a surface of the semiconductor substrate; electrodes disposed on the oxidation layer; and at least one diode connected between at least two of the electrodes. The magnetoresistance device of the present invention has excellent performances of a high field magnetoresistance characteristic and high sensitivity at low magnetic field, and has advantages of low power consumption, simple device structure, low cost and simple manufacturing process.

摘要翻译： 本发明涉及使用半导体衬底的磁阻器件及其制造方法。 磁阻器件包括：半导体衬底; 设置在所述半导体衬底的表面上的氧化层; 设置在氧化层上的电极; 以及连接在至少两个电极之间的至少一个二极管。 本发明的磁电阻器件在低磁场下具有优异的高场磁阻特性和高灵敏度的性能，具有功耗低，器件结构简单，成本低，制造工艺简单等优点。

公开(公告)号：US20120135426A1

公开(公告)日：2012-05-31

申请号：US13245045

申请日：2011-09-26

申请人： Kevin J. Reagan ,  Erik Schaefer ,  Jimin Wang

发明人： Kevin J. Reagan ,  Erik Schaefer ,  Jimin Wang

IPC分类号： G01N33/566 ,  C07K16/18

CPC分类号： C07K16/2863 ,  C07K16/18 ,  C07K2317/34 ,  C12Q1/485 ,  G01N2333/4709

摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.

公开(公告)号：US08484613B2

公开(公告)日：2013-07-09

申请号：US12695908

申请日：2010-01-28

申请人： Harikumar Somakumar ,  Jimin Wang

发明人： Harikumar Somakumar ,  Jimin Wang

IPC分类号： G06F9/44

CPC分类号： G06F11/3604 ,  G06F11/004

摘要： Some embodiments of the present invention provide techniques and systems for optimizing bounds-checking During operation, the system can receive one or more instructions which when executed evaluate a first expression whose value is required to be between a lower bound expression's value and an upper bound expression's value, such that at least one of the following three values is not determinable before execution: the first expression's value, the lower bound expression's value, and the upper bound expression's value. Next, the system can use computer algebra to determine whether the first expression's value is guaranteed to be between the lower bound expression's value and the upper bound expression's value. If the system determines that the first expression's value is not guaranteed to be between the lower bound expression's value and the upper bound expression's value, the system can add bounds-checking instructions to the one or more instructions.

摘要翻译： 本发明的一些实施例提供了用于优化边界检查的技术和系统。在操作期间，系统可以接收一个或多个指令，当执行时，该指令评估第一个表达式，其值需要在下限表达式的值和上限表达式 值，使得以下三个值中的至少一个在执行之前不可确定：第一个表达式的值，下限表达式的值和上限表达式的值。 接下来，系统可以使用计算机代数来确定第一个表达式的值是否保证在下界表达式的值和上限表达式的值之间。 如果系统确定第一个表达式的值不能保证在下限表达式的值和上限表达式的值之间，则系统可以向一个或多个指令添加边界检查指令。

公开(公告)号：US20110185341A1

公开(公告)日：2011-07-28

申请号：US12695908

申请日：2010-01-28

申请人： Harikumar Somakumar ,  Jimin Wang

发明人： Harikumar Somakumar ,  Jimin Wang

IPC分类号： G06F9/44

CPC分类号： G06F11/3604 ,  G06F11/004

摘要： Some embodiments of the present invention provide techniques and systems for optimizing bounds-checking During operation, the system can receive one or more instructions which when executed evaluate a first expression whose value is required to be between a lower bound expression's value and an upper bound expression's value, such that at least one of the following three values is not determinable before execution: the first expression's value, the lower bound expression's value, and the upper bound expression's value. Next, the system can use computer algebra to determine whether the first expression's value is guaranteed to be between the lower bound expression's value and the upper bound expression's value. If the system determines that the first expression's value is not guaranteed to be between the lower bound expression's value and the upper bound expression's value, the system can add bounds-checking instructions to the one or more instructions.

摘要翻译： 本发明的一些实施例提供了用于优化边界检查的技术和系统。在操作期间，系统可以接收一个或多个指令，当执行时，该指令评估第一个表达式，其值需要在下限表达式的值和上限表达式 值，使得以下三个值中的至少一个在执行之前不可确定：第一个表达式的值，下限表达式的值和上限表达式的值。 接下来，系统可以使用计算机代数来确定第一个表达式的值是否保证在下界表达式的值和上限表达式的值之间。 如果系统确定第一个表达式的值不能保证在下限表达式的值和上限表达式的值之间，则系统可以向一个或多个指令添加边界检查指令。

公开(公告)号：US20110165588A1

公开(公告)日：2011-07-07

申请号：US12970250

申请日：2010-12-16

申请人： Kevin J. Reagan ,  Erik Schaefer ,  Jimin Wang

发明人： Kevin J. Reagan ,  Erik Schaefer ,  Jimin Wang

IPC分类号： G01N33/53

CPC分类号： C07K16/2863 ,  C07K16/18 ,  C07K2317/34 ,  C12Q1/485 ,  G01N2333/4709

摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.

摘要翻译： 本发明涉及一种用于测量特定位点蛋白质磷酸化的方法，因此是能够使这些位点磷酸化的酶的蛋白激酶活性的指标。 该方法包括在特定丝氨酸，苏氨酸或酪氨酸残基处的靶蛋白的体外或体内磷酸化，使该蛋白质（非磷酸化）到含有所有试剂的反应混合物，包括磷酸激酶，其允许产生磷酸化形式 蛋白。 通过使磷酸化蛋白质与针对磷酸化位点特异性的抗体接触来测量磷酸化蛋白质。 本发明包括可用于实践本发明方法的抗体。 本发明特别涉及通过磷酸化和去磷酸化修饰的所有蛋白质，如Tau，Rb和EGFR蛋白质所示，以及对Tau，Rb或EGFR蛋白质的磷酸化位点特异的抗体。

公开(公告)号：US07888050B2

公开(公告)日：2011-02-15

申请号：US12179526

申请日：2008-07-24

申请人： Kevin Reagan ,  Erik Schaefer ,  Jimin Wang

发明人： Kevin Reagan ,  Erik Schaefer ,  Jimin Wang

IPC分类号： G01N33/53 ,  G01N33/543

CPC分类号： C07K16/2863 ,  C07K16/18 ,  C07K2317/34 ,  C12Q1/485 ,  G01N2333/4709

摘要： The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins.

摘要翻译： 本发明涉及一种用于测量特定位点蛋白质磷酸化的方法，因此是能够使这些位点磷酸化的酶的蛋白激酶活性的指标。 该方法包括在特定丝氨酸，苏氨酸或酪氨酸残基处的靶蛋白的体外或体内磷酸化，使该蛋白质（非磷酸化）到含有所有试剂的反应混合物，包括磷酸激酶，其允许产生磷酸化形式 蛋白。 通过使磷酸化蛋白质与针对磷酸化位点特异性的抗体接触来测量磷酸化蛋白质。 本发明包括可用于实践本发明方法的抗体。 本发明特别涉及通过磷酸化和去磷酸化修饰的所有蛋白质，如Tau，Rb和EGFR蛋白质所示，以及对Tau，Rb或EGFR蛋白质的磷酸化位点特异的抗体。