公开(公告)号：US08753870B2

公开(公告)日：2014-06-17

申请号：US13605787

申请日：2012-09-06

申请人： Hun-joo Lee ,  Jeong-gun Lee ,  Jeong-woo Choi ,  Yeon-jeong Kim ,  Jong-myeon Park ,  Mi-jeong Song ,  Jin-mi Oh

发明人： Hun-joo Lee ,  Jeong-gun Lee ,  Jeong-woo Choi ,  Yeon-jeong Kim ,  Jong-myeon Park ,  Mi-jeong Song ,  Jin-mi Oh

IPC分类号： C12M1/00 ,  C12Q1/68 ,  G01N33/53 ,  C12M1/34 ,  C12M3/00 ,  G01N30/96 ,  C07H21/02

CPC分类号： G01N33/54306 ,  G01N33/54366

摘要： A device, system, and method for detecting or separating target molecules allowing efficient detection even when only a small amount of target molecules or target cells are included in a sample involving the use of a target molecule linkage portion, a signal production portion, and first and second separation portions.

摘要翻译： 用于检测或分离目标分子的装置，系统和方法，即使当仅使用少量靶分子或靶细胞包括使用靶分子连接部分，信号产生部分和第一 和第二分离部。

公开(公告)号：US20130178543A1

公开(公告)日：2013-07-11

申请号：US13594610

申请日：2012-08-24

申请人： Yeon-jeong KIM ,  You-sun KIM ,  Jeong-gun LEE ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG

发明人： Yeon-jeong KIM ,  You-sun KIM ,  Jeong-gun LEE ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG

IPC分类号： G01N33/574 ,  C12N5/095 ,  C40B30/04 ,  C07K17/00 ,  A61P35/00 ,  A61P35/04 ,  C12N5/09 ,  G01N33/577

CPC分类号： C07K14/705 ,  C12Q1/6881 ,  C12Q1/6886 ,  C12Q2600/158 ,  G01N33/57484

摘要： A method of detecting a cancer stem cell or circulating tumor cell that has undergone epithelial-mesenchymal transition, comprising determining the level of caveolin-1 expressed by a sample cell, and comparing the level of caveolin-1 expressed by the sample cell to a control, wherein higher expression of caveolin-1 by the sample cell indicates that the sample cell is a cancer cell, as well as a method of detecting cancer or metastasis in a subject, and related methods and compositions.

摘要翻译： 一种检测已经经历上皮 - 间质转化的癌症干细胞或循环肿瘤细胞的方法，包括测定由样品细胞表达的窖蛋白-1的水平，并将由样品细胞表达的窖蛋白-1的水平与对照组 其中样品细胞中较高表达的小窝蛋白-1表明样品细胞是癌细胞，以及检测受试者的癌症或转移的方法，以及相关的方法和组合物。

公开(公告)号：US20130149774A1

公开(公告)日：2013-06-13

申请号：US13615267

申请日：2012-09-13

申请人： Min-seok KIM ,  Tae-seok SIM ,  Yeon-jeong KIM ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG ,  Jeong-gun LEE

发明人： Min-seok KIM ,  Tae-seok SIM ,  Yeon-jeong KIM ,  Sang-hyun BAEK ,  Jin-mi OH ,  Hyo-young JEONG ,  Jeong-gun LEE

IPC分类号： G01N27/02 ,  C12M1/36

CPC分类号： G01N15/12 ,  C12M41/36 ,  G01N2015/0065 ,  G01N2015/1081

摘要： A cell counter system includes an inlet via which a fluid containing a plurality of cells inflows; a channel in which the fluid flows; a valve unit, which controls flow of the fluid in the channel; an electrode unit, which is arranged in the channel for measuring impedance for counting a number of the plurality of cells; and an outlet, which is connected to the channel to drain the fluid.

摘要翻译： 细胞计数器系统包括通过其流入含有多个细胞的流体入口的入口; 流体流动的通道; 阀单元，其控制流体在通道中的流动; 电极单元，布置在通道中用于测量用于对多个单元的数量进行计数的阻抗; 和出口，其连接到通道以排出流体。

公开(公告)号：US20130137092A1

公开(公告)日：2013-05-30

申请号：US13605787

申请日：2012-09-06

申请人： Hun-joo LEE ,  Jeong-gun LEE ,  Jeong-woo CHOI ,  Yeon-jeong KIM ,  Jong-myeon PARK ,  Mi-jeong SONG ,  Jin-mi OH

发明人： Hun-joo LEE ,  Jeong-gun LEE ,  Jeong-woo CHOI ,  Yeon-jeong KIM ,  Jong-myeon PARK ,  Mi-jeong SONG ,  Jin-mi OH

IPC分类号： C12M1/34 ,  G01N33/566

CPC分类号： G01N33/54306 ,  G01N33/54366

摘要： A device, system, and method for detecting or separating target molecules allowing efficient detection even when only a small amount of target molecules or target cells are included in a sample involving the use of a target molecule linkage portion, a signal production portion, and first and second separation portions.

摘要翻译： 用于检测或分离目标分子的装置，系统和方法，即使当仅使用少量靶分子或靶细胞包括使用靶分子连接部分，信号产生部分和第一 和第二分离部。

公开(公告)号：US20130017226A1

公开(公告)日：2013-01-17

申请号：US13635868

申请日：2011-03-18

申请人： Young Woo Park ,  Ki Won Jo ,  Srok Ho Yoo ,  Jung Yu ,  Dong Ji Kim ,  Sun-Ha Yoon ,  Eun Jung Song ,  Eun Kyung Lee ,  Jin Mi Oh ,  Kyu Won Cho ,  Mi La Cho ,  Ho Youn Kim ,  Mi Kyung Park ,  Hye Jwa Oh ,  Jin Sil Park ,  Yun Ju Woo ,  Jae Kyeong Byun ,  Jun Geol Ryu

发明人： Young Woo Park ,  Ki Won Jo ,  Srok Ho Yoo ,  Jung Yu ,  Dong Ji Kim ,  Sun-Ha Yoon ,  Eun Jung Song ,  Eun Kyung Lee ,  Jin Mi Oh ,  Kyu Won Cho ,  Mi La Cho ,  Ho Youn Kim ,  Mi Kyung Park ,  Hye Jwa Oh ,  Jin Sil Park ,  Yun Ju Woo ,  Jae Kyeong Byun ,  Jun Geol Ryu

IPC分类号： C07K19/00 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  A61K38/17 ,  A61P19/04 ,  A61P7/06 ,  A61P3/10 ,  A61P19/02 ,  A61P25/00 ,  A61P17/00 ,  A61P21/00 ,  C12N15/62 ,  A61P37/06

CPC分类号： C07K14/525 ,  C07K14/70575 ,  C07K2319/32 ,  Y10S514/814 ,  Y10S514/885 ,  Y10S514/886 ,  Y10S514/903

摘要： The present invention relates to TNFR2-TWEAKR fusion protein, more precisely to TNFR2-TWEAKR fusion protein acting as a double-antagonist to TNF-α and TWEAK, known as major causes of autoimmune arthritis which is one of autoimmune diseases. When the composition comprising TNFR2-TWEAKR fusion protein was treated to Th17 cells, the secretion of the inflammatory cytokine IL-17 was reduced but the secretion of the anti-inflammatory cytokine IL-10 generated in Treg cells was increased. Such effect of TNFR2-TWEAKR fusion protein was far greater than that of a single protein such as TNFR2-Fc or TWEAK-Fc. The TNFR2-TWEAKR fusion protein of the present invention has not only excellent treatment effect on arthritis in CIA mouse model not also excellent treatment effect on autoimmune rheumatoid arthritis by increasing the expression of Treg, the immune suppressive cells. Therefore, the TNFR2-TWEAKR fusion protein of the present invention can be effectively used as an active ingredient for the composition for the prevention and treatment of autoimmune disease.

摘要翻译： 本发明涉及TNFR2-TWEAKR融合蛋白，更确切地说，作为TNF-α和TWEAK的双拮抗剂的TNFR2-TWEAKR融合蛋白，被称为自身免疫性疾病之一的自身免疫性关节炎的主要原因。 当将包含TNFR2-TWEAKR融合蛋白的组合物治疗至Th17细胞时，炎性细胞因子IL-17的分泌减少，但在Treg细胞中产生的抗炎细胞因子IL-10的分泌增加。 TNFR2-TWEAKR融合蛋白的这种作用远远大于单一蛋白如TNFR2-Fc或TWEAK-Fc的这种作用。 本发明的TNFR2-TWEAKR融合蛋白不仅对CIA小鼠模型的关节炎具有优异的治疗作用，而且通过增加免疫抑制细胞Treg的表达，对自身免疫性类风湿性关节炎也不是很好的治疗效果。 因此，本发明的TNFR2-TWEAKR融合蛋白可以有效地用作预防和治疗自身免疫性疾病的组合物的活性成分。

公开(公告)号：US08916525B2

公开(公告)日：2014-12-23

申请号：US13635868

申请日：2011-03-18

申请人： Young Woo Park ,  Ki Won Jo ,  Srok Ho Yoo ,  Jung Yu ,  Dong Jin Kim ,  Sun-Ha Yoon ,  Eun Jung Song ,  Eun Kyung Lee ,  Jin Mi Oh ,  Kyu Won Cho ,  Mi La Cho ,  Ho Youn Kim ,  Mi Kyung Park ,  Hye Jwa Oh ,  Jin Sil Park ,  Yun Ju Woo ,  Jae Kyeong Byun ,  Jun Geol Ryu

发明人： Young Woo Park ,  Ki Won Jo ,  Srok Ho Yoo ,  Jung Yu ,  Dong Jin Kim ,  Sun-Ha Yoon ,  Eun Jung Song ,  Eun Kyung Lee ,  Jin Mi Oh ,  Kyu Won Cho ,  Mi La Cho ,  Ho Youn Kim ,  Mi Kyung Park ,  Hye Jwa Oh ,  Jin Sil Park ,  Yun Ju Woo ,  Jae Kyeong Byun ,  Jun Geol Ryu

IPC分类号： A61K38/17 ,  A61P17/00 ,  A61P19/02 ,  A61P19/04 ,  A61P21/00 ,  A61P25/00 ,  A61P3/10 ,  A61P7/06 ,  C07K14/525 ,  C07K14/705

CPC分类号： C07K14/525 ,  C07K14/70575 ,  C07K2319/32 ,  Y10S514/814 ,  Y10S514/885 ,  Y10S514/886 ,  Y10S514/903

摘要： The present invention relates to TNFR2-TWEAKR fusion protein, more precisely to TNFR2-TWEAKR fusion protein acting as a double-antagonist to TNF-α and TWEAK, known as major causes of autoimmune arthritis which is one of autoimmune diseases. When the composition comprising TNFR2-TWEAKR fusion protein was treated to Th17 cells, the secretion of the inflammatory cytokine IL-17 was reduced but the secretion of the anti-inflammatory cytokine IL-10 generated in Treg cells was increased. Such effect of TNFR2-TWEAKR fusion protein was far greater than that of a single protein such as TNFR2-Fc or TWEAK-Fc. The TNFR2-TWEAKR fusion protein of the present invention has not only excellent treatment effect on arthritis in CIA mouse model not also excellent treatment effect on autoimmune rheumatoid arthritis by increasing the expression of Treg, the immune suppressive cells. Therefore, the TNFR2-TWEAKR fusion protein of the present invention can be effectively used as an active ingredient for the composition for the prevention and treatment of autoimmune disease.

摘要翻译： 本发明涉及TNFR2-TWEAKR融合蛋白，更确切地说，作为TNF-α和TWEAK的双拮抗剂的TNFR2-TWEAKR融合蛋白，被称为自身免疫性疾病之一的自身免疫性关节炎的主要原因。 当将包含TNFR2-TWEAKR融合蛋白的组合物治疗至Th17细胞时，炎性细胞因子IL-17的分泌减少，但在Treg细胞中产生的抗炎细胞因子IL-10的分泌增加。 TNFR2-TWEAKR融合蛋白的这种作用远远大于单一蛋白如TNFR2-Fc或TWEAK-Fc的这种作用。 本发明的TNFR2-TWEAKR融合蛋白不仅对CIA小鼠模型的关节炎具有优异的治疗作用，而且通过增加免疫抑制细胞Treg的表达，对自身免疫性类风湿性关节炎也不是很好的治疗效果。 因此，本发明的TNFR2-TWEAKR融合蛋白可以有效地用作预防和治疗自身免疫性疾病的组合物的活性成分。