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    DEVICE AND METHOD FOR HIGH THROUGHPUT SCREENING OF CRYSTALLIZATION CONDITIONS IN A VAPOR DIFFUSION ENVIRONMENT
    7.
    发明申请
    DEVICE AND METHOD FOR HIGH THROUGHPUT SCREENING OF CRYSTALLIZATION CONDITIONS IN A VAPOR DIFFUSION ENVIRONMENT 审中-公开
    蒸汽扩散环境中结晶条件高通量筛选的装置和方法

    公开(公告)号：US20090111711A1

    公开(公告)日：2009-04-30

    申请号：US12109723

    申请日：2008-04-25

    申请人： Francis A. Lewandowski ,  Joseph Daniel Kwasnoski ,  John C. Spurlino

    发明人： Francis A. Lewandowski ,  Joseph Daniel Kwasnoski ,  John C. Spurlino

    IPC分类号： C40B30/10 ,  C40B60/12

    CPC分类号： C30B7/00 ,  B01L3/06 ,  B01L3/50853 ,  B01L2200/0689 ,  B01L2300/0654 ,  B01L2300/0829 ,  C30B29/58

    摘要： A high-density high-throughput microplate and methods for simultaneously screening a plurality of protein crystallization solutions and for producing diffraction quality protein crystals in a vapor-diffusion environment are disclosed. The microplate has defined side-by-side paired chambers of equal size, wherein the side-by-side paired chambers have a maximum volume of about 8 μl, and wherein the paired chambers have a vapor channel, therein providing vapor exchange between the side-by-side paired chambers. The microplate further includes a membrane to seal the surface of the microplate. The microplate is adapted to receive a crystallization solution in one of the side-by-side paired chambers and a protein solution in the other of the side-by-side paired chambers, wherein the protein solution and the crystallization solution interact via a vapor diffusion process, which enables the formation of protein crystals within the chamber that contains the protein solution.

    摘要翻译： 公开了一种高密度高通量微孔板和用于同时筛选多种蛋白质结晶溶液和用于在蒸气扩散环境中产生衍射质量蛋白质晶体的方法。 微孔板限定了相同尺寸的并排成对的室，其中并排配对室具有约8mul的最大体积，并且其中成对室具有蒸气通道，其中在侧面之间提供蒸气交换 - 双面配对室。 微孔板还包括密封微孔板表面的膜。 微孔板适于在并排配对室之一中接收结晶溶液，并且在另一个并排配对室中接收蛋白质溶液，其中蛋白质溶液和结晶溶液经由蒸气扩散相互作用 方法，其使得能够在包含蛋白质溶液的室内形成蛋白质晶体。