公开(公告)号：US20120077270A1

公开(公告)日：2012-03-29

申请号：US13251627

申请日：2011-10-03

Applicant: Stephen Hart ,  Simak Ali ,  Boris Tumi PuFong ,  Andrew C.G. Porter ,  Laki Buluwela ,  Satu Vainikka ,  John David Jenkinson ,  Patrick Kanda

Inventor： Stephen Hart ,  Simak Ali ,  Boris Tumi PuFong ,  Andrew C.G. Porter ,  Laki Buluwela ,  Satu Vainikka ,  John David Jenkinson ,  Patrick Kanda

IPC: C12N5/071 ,  C07K1/00 ,  C07K14/00 ,  C12N5/04 ,  C12N1/20

CPC classification number: C12N15/113 ,  C12N15/1138 ,  C12N15/63 ,  C12N2310/15 ,  C12N2310/335 ,  C12N2310/336 ,  C12N2310/3513

Abstract: A method for suppressing the expression of a selected gene in a cell, the method comprising introducing into the cell a molecule comprising (1) a nucleic acid binding portion which binds to a site or associated with the selected gene which site is present in a genome and (2) an expression repressor portion, wherein the nucleic acid binding portion comprises an oligonucleotide or oligonucleotide mimic or analogue, and wherein the repressor portion comprises a polypeptide or peptidomimetic. Molecules for use in the methods of the invention are provided. The repressor may be a portion of a histone deacetylase or DNA methylase or polypeptide capable of recruiting a histone deacetylase or DNA methylase.

Abstract translation: 一种用于抑制细胞中所选基因表达的方法，所述方法包括向细胞中引入包含（1）结合位点或与选定基因相关的核酸结合部分的分子，该位点存在于基因组中 和（2）表达阻遏物部分，其中所述核酸结合部分包含寡核苷酸或寡核苷酸模拟物或类似物，并且其中所述阻遏物部分包含多肽或肽模拟物。 提供了用于本发明方法的分子。 阻遏物可以是能够募集组蛋白脱乙酰酶或DNA甲基化酶的组蛋白脱乙酰酶或DNA甲基化酶或多肽的一部分。

公开(公告)号：US20090082250A1

公开(公告)日：2009-03-26

申请号：US12152175

申请日：2008-05-13

Applicant: Stephen Hart ,  Simak Ali ,  Boris Tumi PuFong ,  Andrew C. G. Porter ,  Laki Buluwela ,  Satu Vainikka ,  John David Jenkinson ,  Patrick Kanda

Inventor： Stephen Hart ,  Simak Ali ,  Boris Tumi PuFong ,  Andrew C. G. Porter ,  Laki Buluwela ,  Satu Vainikka ,  John David Jenkinson ,  Patrick Kanda

IPC: C12N15/11 ,  A61K38/02 ,  A01H1/06 ,  G01N33/53 ,  C12Q1/68

CPC classification number: C12N15/113 ,  C12N15/1138 ,  C12N15/63 ,  C12N2310/15 ,  C12N2310/335 ,  C12N2310/336 ,  C12N2310/3513

Abstract: A method for suppressing the expression of a selected gene in a cell the method comprising introducing into the cell a molecule comprising (1) a nucleic acid binding portion which binds to a site or associated with the selected gene which site is present in a genome and (2) an expression repressor portion, wherein the nucleic acid binding portion comprises an oligonucleotide or oligonucleotide mimic or analogue, and wherein the repressor portion comprises a polypeptide or peptidomimetic. Molecules for use in the methods of the invention are provided. The repressor may be a portion of a histone deacetylase or DNA methylase or polypeptide capable of recruiting a histone deacetylase or DNA methylase.

Abstract translation: 一种抑制细胞中所选择的基因表达的方法，包括向细胞中引入分子，所述分子包含（1）核酸结合部分，所述核酸结合部分结合位点或与所选基因相关联，所述基因存在于基因组中， （2）表达阻遏物部分，其中所述核酸结合部分包含寡核苷酸或寡核苷酸模拟物或类似物，并且其中所述阻遏物部分包含多肽或肽模拟物。 提供了用于本发明方法的分子。 阻遏物可以是能够募集组蛋白脱乙酰酶或DNA甲基化酶的组蛋白脱乙酰酶或DNA甲基化酶或多肽的一部分。