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公开(公告)号：US08480545B2
公开(公告)日：2013-07-09
申请号：US13346597
申请日：2012-01-09
申请人： Colin Irving , John J. Harrington
发明人： Colin Irving , John J. Harrington
IPC分类号： A63B22/06
CPC分类号： A63B22/0605 , A63B21/015 , A63B21/225 , A63B21/4034 , A63B21/4035 , A63B22/0046 , A63B23/02 , A63B23/0405 , A63B23/0476 , A63B2022/0641 , A63B2022/0658 , A63B2225/09 , A63B2225/093
摘要： An apparatus permitting a user to perform a simulated bicycling exercise is provided. The design includes a horizontal base, a lower front pivoting point, and user supporting hardware collectively adjoining pedals, a seat configured to support the user, and handlebars, the user supporting hardware provided above the horizontal base and interfacing with the lower front mounting point. The lower front pivoting point and the user supporting hardware include a multiple component resistive element arrangement and collectively define an axis of rotation forming an angle with the horizontal base of 30 to 45 degrees, sloping upward in a rearward direction from the front pivoting point to the user supporting hardware. When the user is seated on the seat and leans in one direction, such leaning causes the user supporting hardware, seat, and handlebars to rotate in the one direction about the axis of rotation.
摘要翻译： 提供允许用户进行模拟骑自行车运动的装置。 该设计包括水平基座，下部前部枢转点和用户支撑硬件集体毗邻的踏板，被配置为支撑用户的座椅和把手，提供在水平基座上方的用户支撑硬件并且与下部前部安装点对接。 下部前枢转点和用户支撑硬件包括多分量电阻元件布置，并且共同地限定与水平底座成30度到45度角的旋转轴线，从前方枢转点向后方向上倾斜 用户支持硬件。 当用户坐在座椅上并沿着一个方向倾斜时，这种倾斜使用户支撑五金，座椅和把手围绕旋转轴线在一个方向上旋转。
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公开(公告)号：US07842792B2
公开(公告)日：2010-11-30
申请号：US10331329
申请日：2002-12-30
CPC分类号： C07K14/705 , A61K48/00 , C07K2319/00 , C12N9/003 , C12N15/1096 , C12N15/63 , C12N15/67 , C12N15/85 , C12N15/90 , C12N2800/107 , C12N2800/108 , C12N2800/204 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. In another embodiment, the expression of the endogenous gene may be further increased by co-integration of one or more amplifiable markers, and selecting for increased copies of the one or more amplifiable markers located on the integrated vector. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration.
摘要翻译： 本发明一般涉及通过原位重组方法激活基因表达或引起基因过度表达。 本发明还通常涉及用于在细胞中以比通常在细胞中发现的那些更高的水平表达内源基因的方法。 在本发明的一个实施方案中，内源基因的表达在通过激活基因表达的调节序列的非同源或非法重组整合入细胞后被激活或增加。 在另一个实施方案中，可以通过一个或多个可扩增标记的共同整合以及选择位于整合载体上的一个或多个可扩增标记物的增加拷贝来进一步增加内源基因的表达。 本发明还提供了鉴定，激活，分离和/或表达不能被现有方法发现的基因的方法，因为不需要靶序列进行整合。
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公开(公告)号：US20090048076A1
公开(公告)日：2009-02-19
申请号：US11893634
申请日：2007-08-17
CPC分类号： A63B22/0605 , A63B21/00076 , A63B21/015 , A63B21/225 , A63B21/4034 , A63B21/4035 , A63B22/0015 , A63B23/0476 , A63B2022/0641 , A63B2022/0658 , A63B2071/0063 , A63B2225/09 , A63B2225/093
摘要： An apparatus permitting a user to perform a simulated bicycling exercise is provided. The design includes a frame and a first mounting point and a second mounting point configured to maintain the frame. A seat is connected to the frame and configured to support the user. A wheel is positioned in association with said frame and pedals configured to interact with the wheel, and the frame is configured to pivot about the first mounting point and second mounting point in response to leaning by the user. Handlebars may be provided that enable further force application and enhance the leaning or pivoting in the bicycle riding simulation experience.
摘要翻译： 提供允许用户进行模拟骑自行车运动的装置。 该设计包括框架和第一安装点以及被配置为保持框架的第二安装点。 座椅连接到框架并配置为支持用户。 与所述框架相关联的轮子和被配置为与车轮相互作用的踏板定位，并且框架构造成响应于使用者的倾斜而围绕第一安装点和第二安装点枢转。 可以提供手把，其能够进一步施加力并增强骑自行车骑行模拟经验中的倾斜或枢转。
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公开(公告)号：US07419828B2
公开(公告)日：2008-09-02
申请号：US09484331
申请日：2000-01-18
申请人： John J. Harrington
发明人： John J. Harrington
CPC分类号： C07K14/705 , A61K48/00 , C07K2319/00 , C12N9/003 , C12N15/1096 , C12N15/63 , C12N15/67 , C12N15/85 , C12N15/90 , C12N2800/107 , C12N2800/108 , C12N2800/204 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. The invention is specifically directed to methods of drug discovery using the activated gene products for compound testing.
摘要翻译： 本发明一般涉及通过原位重组方法激活基因表达或引起基因过度表达。 本发明还通常涉及用于在细胞中以比通常在细胞中发现的那些更高的水平表达内源基因的方法。 在本发明的一个实施方案中，内源基因的表达在通过激活基因表达的调节序列的非同源或非法重组整合入细胞后被激活或增加。 本发明还提供了鉴定，激活，分离和/或表达不能被现有方法发现的基因的方法，因为不需要靶序列进行整合。 本发明具体涉及使用活化基因产物进行化合物测试的药物发现方法。
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公开(公告)号：US07033782B2
公开(公告)日：2006-04-25
申请号：US09760897
申请日：2001-01-17
申请人： John J. Harrington
发明人： John J. Harrington
CPC分类号： C07K14/705 , A61K48/00 , C07K2319/00 , C12N9/003 , C12N15/1096 , C12N15/63 , C12N15/67 , C12N15/85 , C12N15/90 , C12N2800/107 , C12N2800/108 , C12N2800/204 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. Thus, by the present invention, endogenous genes, including those associated with human disease and development, may be activated and isolated without prior knowledge of the sequence, structure, function, or expression profile of the genes.
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公开(公告)号：US06670185B1
公开(公告)日：2003-12-30
申请号：US09479123
申请日：2000-01-07
IPC分类号： C12N1509
CPC分类号： C07K14/705 , A61K48/00 , C07K2319/00 , C12N9/003 , C12N15/1096 , C12N15/63 , C12N15/67 , C12N15/85 , C12N15/90 , C12N2800/107 , C12N2800/108 , C12N2800/204 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. The invention also provides methods for isolation of nucleic acid molecules (particularly cDNA molecules) encoding a variety of proteins. Thus, by the present invention, endogenous genes, including those associated with human disease and development, may be activated and isolated without prior knowledge of the sequence, structure, function, or expression profile of the genes.
摘要翻译： 本发明一般涉及通过原位重组方法激活基因表达或引起基因过度表达。 本发明还通常涉及用于在细胞中以比通常在细胞中发现的那些更高的水平表达内源基因的方法。 在本发明的一个实施方案中，内源基因的表达在通过激活基因表达的调节序列的非同源或非法重组整合入细胞后被激活或增加。 本发明还提供了鉴定，激活，分离和/或表达不能被现有方法发现的基因的方法，因为不需要靶序列进行整合。 本发明还提供了分离编码多种蛋白质的核酸分子（特别是cDNA分子）的方法。 因此，通过本发明，可以在没有基因的序列，结构，功能或表达谱的事先知识的情况下激活和分离内源基因，包括与人类疾病和发育相关的内源基因。
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公开(公告)号：US06541221B1
公开(公告)日：2003-04-01
申请号：US09481282
申请日：2000-01-11
IPC分类号： C12N1500
CPC分类号： C07K14/705 , A61K48/00 , C07K2319/00 , C12N9/003 , C12N15/1096 , C12N15/63 , C12N15/67 , C12N15/85 , C12N15/90 , C12N2800/107 , C12N2800/108 , C12N2800/204 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Expression of an endogenous gene is activated or increased following integration into a cell, by non-homologous or illegitimate recombination, of (1) an enhancer sequence that activates expression of the gene and (2) a sequence that encodes an amplifiable marker. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. The invention also provides cells containing the enhancer and amplifiable marker sequence and expressing increased amounts of a desired gene. The invention also provides methods for the isolation of nucleic acid molecules (particularly cDNA molecules) encoding a variety of proteins, including transmembrane proteins, and for the isolation of cells expressing such proteins. Thus, by the present invention, endogenous genes, including those associated with human disease and development, may be activated and isolated without prior knowledge of the sequence, structure, function, or expression profile of the genes.
摘要翻译： 通过非同源或非法重组融合到细胞中，内源基因的表达被激活或增加，（1）激活基因表达的增强子序列和（2）编码可扩增标记的序列。 本发明还提供了鉴定，激活，分离和/或表达不能被现有方法发现的基因的方法，因为不需要靶序列进行整合。 本发明还提供含有增强子和可扩增标记序列并表达增加量的所需基因的细胞。 本发明还提供了分离编码多种蛋白质（包括跨膜蛋白）的核酸分子（特别是cDNA分子）以及分离表达这种蛋白质的细胞的方法。 因此，通过本发明，可以在没有基因的序列，结构，功能或表达谱的事先知识的情况下激活和分离内源基因，包括与人类疾病和发育相关的内源基因。
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公开(公告)号：US06524818B1
公开(公告)日：2003-02-25
申请号：US09484997
申请日：2000-01-18
IPC分类号： C12N1500
CPC分类号： C07K14/705 , A61K48/00 , C07K2319/00 , C12N9/003 , C12N15/1096 , C12N15/63 , C12N15/67 , C12N15/85 , C12N15/90 , C12N2800/107 , C12N2800/108 , C12N2800/204 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. In another embodiment, the expression of the endogenous gene may be further increased by co-integration of one or more amplifiable markers, and selecting for increased copies of the one or more amplifiable markers located on the integrated vector. In another embodiment, the invention is directed to activation of endogenous genes by non-targeted integration of specialized activation vectors, which are provided by the invention, into the genome of a host cell. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. The invention also provides methods for isolation of nucleic acid molecules (particularly cDNA molecules) encoding a variety of proteins, including transmembrane proteins, and for isolation of cells expressing such transmembrane proteins which may be heterologous transmembrane proteins. The invention also is directed to isolated genes, gene products, nucleic acid molecules, to compositions comprising such genes, gene products and nucleic acid molecules, and to vectors and host cells comprising such genes and nucleic acid molecules, that may be used in a variety of therapeutic and diagnostic applications. Thus, by the present invention, endogenous genes, including those associated with human disease and development, may be activated and isolated without prior knowledge of the sequence, structure, function, or expression profile of the genes.
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公开(公告)号：US06410266B1
公开(公告)日：2002-06-25
申请号：US09479122
申请日：2000-01-07
IPC分类号： C12N1563
CPC分类号： C07K14/705 , A61K48/00 , C07K2319/00 , C12N9/003 , C12N15/1096 , C12N15/63 , C12N15/67 , C12N15/85 , C12N15/90 , C12N2800/107 , C12N2800/108 , C12N2800/204 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. In another embodiment, the expression of the endogenous gene may be further increased by co-integration of one or more amplifiable markers, and selecting for increased copies of the one or more ampliflable markers located on the integrated vector. In another embodiment, the invention is directed to activation of endogenous genes by non-targeted integration of specialized activation vectors, which are provided by the invention, into the genome of a host cell. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. The invention also provides methods for isolation of nucleic acid molecules (particularly cDNA molecules) encoding a variety of proteins, including transmembrane proteins, and for isolation of cells expressing such transmembrane proteins which maybe heterologous transmembrane proteins. The invention also is directed to isolated genes, gene products, nucleic acid molecules, to compositions comprising such genes, gene products and nucleic acid molecules, and to vectors and host cells comprising such genes and nucleic acid molecules, that may be used in a variety of therapeutic and diagnostic applications. Thus, by the present invention, endogenous genes, including those associated with human disease and development, may be activated and isolated without prior knowledge of the sequence, structure, function, or expression profile of the genes.
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公开(公告)号：US07316923B1
公开(公告)日：2008-01-08
申请号：US09484895
申请日：2000-01-18
CPC分类号： C12N15/63 , A61K48/00 , C07K2319/00 , C12N15/1096 , C12N15/67 , C12N15/85 , C12N15/90 , C12N2800/108 , C12N2800/204 , C12N2800/60 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： The present invention is directed generally to activating gene expression or causing over-expression of a gene by recombination methods in situ. The invention also is directed generally to methods for expressing an endogenous gene in a cell at levels higher than those normally found in the cell. In one embodiment of the invention, expression of an endogenous gene is activated or increased following integration into the cell, by non-homologous or illegitimate recombination, of a regulatory sequence that activates expression of the gene. In another embodiment, the expression of the endogenous gene may be further increased by co-integration of one or more amplifiable markers, and selecting for increased copies of the one or more amplifiable markers located on the integrated vector. In another embodiment, the invention is directed to activation of endogenous genes by non-targeted integration of specialized activation vectors, which are provided by the invention, into the genome of a host cell. The invention also provides methods for the identification, activation, isolation, and/or expression of genes undiscoverable by current methods since no target sequence is necessary for integration. The invention also provides methods for isolation of nucleic acid molecules (particularly cDNA molecules) encoding a variety of proteins, including transmembrane proteins, and for isolation of cells expressing such transmembrane proteins which may be heterologous transmembrane proteins. The invention also is directed to isolated genes, gene products, nucleic acid molecules, to compositions comprising such genes, gene products and nucleic acid molecules, and to vectors and host cells comprising such genes and nucleic acid molecules, that may be used in a variety of therapeutic and diagnostic applications. Thus, by the present invention, endogenous genes, including those associated with human disease and development, may be activated and isolated without prior knowledge of the sequence, structure, function, or expression profile of the genes.
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