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    Methods for the identification and characterization of reticulocytes in whole blood
    2.
    发明授权
    Methods for the identification and characterization of reticulocytes in whole blood 失效
    全血中网织红细胞的鉴定和表征方法

    公开(公告)号：US5350695A

    公开(公告)日：1994-09-27

    申请号：US802593

    申请日：1991-12-05

    申请人： Gregory M. Colella ,  Daniel Ben-David ,  Albert Cupo ,  Sophie S. Fan ,  Gena Fischer ,  Grace E. Martin ,  Leonard Ornstein

    发明人： Gregory M. Colella ,  Daniel Ben-David ,  Albert Cupo ,  Sophie S. Fan ,  Gena Fischer ,  Grace E. Martin ,  Leonard Ornstein

    IPC分类号： G01N15/14 ,  G01N1/30 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N33/5094 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

    摘要： Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include organic cationic dyes for staining the reticulocytes in the blood sample and buffer solutions for maintaining a pH of about 6 to about 9. The dyes may be the blue absorption dyes Oxazine 750 or New Methylene Blue. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration. One method also includes adjusting the measured absorption signals for pseudo-absorption.

    摘要翻译： 表征和区分网织红细胞和成熟红细胞的方法使用试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。染料可以是蓝色吸收染料恶嗪 750或新亚甲基蓝。 当用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂时，并且将试剂组合物和全血样品混合物通过流式细胞仪的感测区域，测量每个细胞散射和吸收的光， 红细胞可以与网织红细胞区分开，并从测定的细胞 - 细胞计算出网织红细胞或红细胞的体积，血红蛋白浓度和每种网织红细胞或红细胞的血红蛋白含量，平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白， 细胞内体积和血红蛋白浓度。 一种方法还包括调整测量的吸收信号用于伪吸收。

    Methods for the identification and characterization of reticulocytes in whole blood
    5.
    发明授权
    Methods for the identification and characterization of reticulocytes in whole blood 失效
    全血中网织红细胞的鉴定和表征方法

    公开(公告)号：US5360739A

    公开(公告)日：1994-11-01

    申请号：US802585

    申请日：1991-12-05

    申请人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    发明人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N15/1459 ,  G01N33/5094 ,  G01N1/38 ,  G01N15/1463 ,  G01N2015/0076 ,  G01N2015/1486 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

    摘要： Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include an organic cationic dye for staining the reticulocytes in the blood sample and a buffer solution for maintaining a pH of about 6 to about 9. The dyes may be the red excitable fluorescent dye Oxazine 750, or the blue excitable fluorescent dyes Acridine Orange or derivatives of Acridine Orange. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered through at least one angular interval and that fluoresced by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

    摘要翻译： 表征和区分网织红细胞和成熟红细胞的方法使用试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。染料可以是红色可激发 荧光染料Oxazine 750，或蓝色可激发荧光染料吖啶橙或吖啶橙衍生物。 当用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂时，试剂组合物和全血样品混合物通过流式细胞计数器的感测区域时，散射在至少一个角度间隔的光， 测量每个细胞发荧光的红细胞，网织红细胞和网织红细胞或红细胞的体积，血红蛋白浓度和血红蛋白含量以及网织红细胞的平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白和/ 或红细胞从测量的细胞逐个体积和血红蛋白浓度计算。

    Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood
    7.
    发明授权
    Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood 失效
    试剂组合物及其在全血中鉴定和表征网织红细胞的用途

    公开(公告)号：US5411891A

    公开(公告)日：1995-05-02

    申请号：US961582

    申请日：1992-10-15

    申请人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    发明人： Sophie S. Fan ,  Daniel Ben-David ,  Gregory M. Colella ,  Albert Cupo ,  Gena Fischer ,  Leonard Ornstein

    IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N21/27 ,  G01N21/64 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/50 ,  G01N33/72 ,  G01N33/80

    CPC分类号： G01N33/80 ,  G01N1/30 ,  G01N15/1459 ,  G01N33/5094 ,  G01N1/38 ,  G01N15/1463 ,  G01N2015/0076 ,  G01N2015/1486 ,  Y10S436/80 ,  Y10T436/10 ,  Y10T436/101666

    摘要： A reagent composition which include an organic cationic dye for staining the reticulocytes in the blood sample and a buffer solution for maintaining a pH of about 6 to about 9 is provided. The dyes may be the red excitable fluorescent dye Oxazine 750, or the blue excitable fluorescent dyes Acridine Orange or derivatives of Acridine Orange. A zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes. The reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered through at least one angular interval and that fluoresced by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

    摘要翻译： 提供了一种试剂组合物，其包括用于染色血液样品中的网织红细胞的有机阳离子染料和用于保持约6至约9的pH的缓冲溶液。 染料可以是红色可激发荧光染料Oxazine 750，或蓝色可激发荧光染料吖啶橙或吖啶橙衍生物。 用于网织红细胞和红细胞的等容体积的试剂组合物中包含两性离子表面活性剂。 试剂组合物和全血样品混合物通过流式细胞仪的感测区域，测量散布在至少一个角度间隔内并由每个细胞发荧光的光，红细胞可与网织红细胞和体积，血红蛋白浓度 并根据测定的细胞间细胞体积和血红蛋白浓度计算网织红细胞或红细胞的血红蛋白含量，以及网织红细胞和/或红细胞的平均细胞体积，平均红细胞血红蛋白浓度和平均细胞血红蛋白。

    METHOD AND LONG-TERM STABLE BICARBONATE-CONTAINING DILUENT COMPOSITION, AND STORAGE MEANS THEREFOR, FOR REDUCING OR REVERSING AERATION INDUCED CELL SHRINKAGE AND STORAGE INDUCED CELL SWELLING OF A WHOLE BLOOD SAMPLE
    8.
    发明授权
    METHOD AND LONG-TERM STABLE BICARBONATE-CONTAINING DILUENT COMPOSITION, AND STORAGE MEANS THEREFOR, FOR REDUCING OR REVERSING AERATION INDUCED CELL SHRINKAGE AND STORAGE INDUCED CELL SWELLING OF A WHOLE BLOOD SAMPLE 有权
    方法和长期稳定的含二碳酸酯的稀释剂组合物及其储存方法，用于减少或逆转诱导的细胞收缩和储存诱导的全血样品的细胞裂解

    公开(公告)号：US06468732B1

    公开(公告)日：2002-10-22

    申请号：US09819263

    申请日：2001-03-28

    申请人： Michael J. Malin ,  Leonard Ornstein

    发明人： Michael J. Malin ,  Leonard Ornstein

    IPC分类号： A01N102

    CPC分类号： A01N1/02 ,  A01N1/0226 ,  Y10T436/107497 ,  Y10T436/108331

    摘要： The invention provides a bicarbonate-containing, crosslinker-free reagent composition which reverses and/or reduces sample handling effects, such as aeration-induced shrinkage and storage-induced swelling of cells in a whole blood sample, particularly for a blood sample requiring repeated sampling and/or subjected to long-term storage. The reagent composition serves as a diluent medium for a blood sample, or an aliquot or portion thereof. The pH of the reagent is in the range of about 7.2 to about 7.5, preferably 7.3. The stability of the pH 7.2-7.5 bicarbonate-containing reagent over time is maintained by storing the reagent in a flexible collapsible container which is impermeable to carbon dioxide and is comprised of a multilayered flexible material, preferably plastic, and attachable to a hematology analyzer.

    摘要翻译： 本发明提供了一种含碳酸氢盐的不含交联剂的试剂组合物，其反转和/或降低样品处理效果，例如通气诱导的收缩和储存诱导的全血样品中的细胞肿胀，特别是需要重复取样的血液样品 和/或经受长期储存。 试剂组合物用作血液样品或等分试样或其部分的稀释介质。 试剂的pH在约7.2至约7.5的范围内，优选7.3。 通过将试剂储存在二氧化碳不可渗透的柔性可折叠容器中并由多层柔性材料（优选塑料）组成并且可连接到血液分析仪来维持pH 7.2-7.5含碳酸氢盐试剂随时间的稳定性。

    Method for discriminating surface stained lymphocytes
    9.
    发明授权
    Method for discriminating surface stained lymphocytes 失效
    辨别表面染色淋巴细胞的方法

    公开(公告)号：US5122453A

    公开(公告)日：1992-06-16

    申请号：US188803

    申请日：1988-05-03

    申请人： Grace E. Martin ,  Robert J. Kanter ,  Young R. Kim ,  Leonard Ornstein ,  Hudson R. Ansley

    发明人： Grace E. Martin ,  Robert J. Kanter ,  Young R. Kim ,  Leonard Ornstein ,  Hudson R. Ansley

    IPC分类号： G01N33/569

    CPC分类号： G01N33/56972 ,  Y10S436/805 ,  Y10S436/825

    摘要： A flow cytometry method for reproducibly detecting and counting a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color-producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.

    摘要翻译： 一种用于可重复地检测和计数白细胞悬浮液或全血样品中感兴趣的淋巴细胞群的流式细胞术方法，其中红细胞随后被裂解。 将悬浮液（或样品）与包含天然的，携带附着的酶或生物素或其它标记的一级抗体和固定剂的试剂以任何顺序组合。 当酶不连接时，酶与第一抗体特异性地偶联。 将固定的悬浮液与产生色素的酶 - 细胞化学试剂反应。 现在包括染色和未染色的固定细胞的悬浮液通过流式细胞仪，并且基于它们的光散射和光吸收性质对细胞进行表征和计数。