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公开(公告)号:US07575865B2
公开(公告)日:2009-08-18
申请号:US11195254
申请日:2005-08-01
申请人: John H. Leamon , Keith E. McDade , Joseph M. Fierro , James R. Knight , Jaran Charumilind , Eugene W. Myers, Jr. , John W. Simpson , Greg A. Volkmer
发明人: John H. Leamon , Keith E. McDade , Joseph M. Fierro , James R. Knight , Jaran Charumilind , Eugene W. Myers, Jr. , John W. Simpson , Greg A. Volkmer
CPC分类号: B01L3/502761 , B01L3/5027 , B01L3/5085 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G06F19/22
摘要: An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译: 本文提供了用于进行快速DNA测序的装置和方法,例如基因组测序。 该方法包括以下步骤:制备用于基因组测序的样品DNA,以代表性方式扩增所制备的DNA,并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。