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公开(公告)号：US07575865B2
公开(公告)日：2009-08-18
申请号：US11195254
申请日：2005-08-01
申请人： John H. Leamon , Keith E. McDade , Joseph M. Fierro , James R. Knight , Jaran Charumilind , Eugene W. Myers, Jr. , John W. Simpson , Greg A. Volkmer
发明人： John H. Leamon , Keith E. McDade , Joseph M. Fierro , James R. Knight , Jaran Charumilind , Eugene W. Myers, Jr. , John W. Simpson , Greg A. Volkmer
CPC分类号： B01L3/502761 , B01L3/5027 , B01L3/5085 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G06F19/22
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译： 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。
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公开(公告)号：US20110251078A1
公开(公告)日：2011-10-13
申请号：US13044330
申请日：2011-03-09
申请人： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
发明人： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
IPC分类号： C40B30/00
CPC分类号： C12Q1/6874 , C12Q2523/303 , C12Q2565/131 , C12Q2525/204 , C12Q2521/101 , C12Q2535/122
摘要： The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.
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公开(公告)号：US20100035252A1
公开(公告)日：2010-02-11
申请号：US12319140
申请日：2008-12-31
申请人： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. Van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
发明人： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. Van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
CPC分类号： C12Q1/6874 , C12Q2523/303 , C12Q2565/131 , C12Q2525/204 , C12Q2521/101 , C12Q2535/122
摘要： The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.
摘要翻译： 本发明提供了用于测序经受力的核酸的装置及其使用方法，并因此在张力下考虑。 所述方法可以采用但不依赖于外源可检测标记的核苷酸的引入。
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公开(公告)号：US20090011959A1
公开(公告)日：2009-01-08
申请号：US12154901
申请日：2008-05-27
IPC分类号： C40B50/14
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; ligating the fragments to universal adaptors; performing strand displacement and extension of the nicked fragments; purifying the double-stranded ligation products; capturing the double-stranded ligation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.
摘要翻译： 本发明涉及产生用于扩增和测序反应的单链DNA文库的方法。 在各方面，所公开的方法包括：片段化DNA; 抛光片段的末端; 将碎片连接到通用适配器; 进行缝合位移和切口碎片的延伸; 纯化双链结合产物; 将双链结合产物捕获到固体支持物上; 并分离单链DNA文库片段，并将这些片段结合到另一种固体支持物上。
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公开(公告)号：US07323305B2
公开(公告)日：2008-01-29
申请号：US10767779
申请日：2004-09-22
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
摘要翻译： 本文提供了用于进行快速DNA测序的装置和方法，例如基因组测序。 该方法包括以下步骤：制备用于基因组测序的样品DNA，以代表性方式扩增所制备的DNA，并仅在一个引物杂交步骤对经扩增的DNA进行多次测序反应。
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公开(公告)号：US08906617B2
公开(公告)日：2014-12-09
申请号：US13044361
申请日：2011-03-09
申请人： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. Van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
发明人： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. Van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
CPC分类号： C12Q1/6874 , C12Q2523/303 , C12Q2565/131 , C12Q2525/204 , C12Q2521/101 , C12Q2535/122
摘要： The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.
摘要翻译： 本发明提供了用于测序经受力的核酸的装置及其使用方法，并因此在张力下考虑。 所述方法可以采用但不依赖于外源可检测标记的核苷酸的引入。
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公开(公告)号：US08748102B2
公开(公告)日：2014-06-10
申请号：US13033240
申请日：2011-02-23
申请人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
发明人： Jan Berka , Yi-Ju Chen , John H. Leamon , Steve Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
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公开(公告)号：US20110251110A1
公开(公告)日：2011-10-13
申请号：US13044352
申请日：2011-03-09
申请人： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. Van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
发明人： Jonathan M. Rothberg , John H. Leamon , John F. Davidson , Antoine M. Van Oijen , Wolfgang Hinz , Melville Davey , Bradley Hann , Jonathan Schultz
CPC分类号： C12Q1/6874 , C12Q2523/303 , C12Q2565/131 , C12Q2525/204 , C12Q2521/101 , C12Q2535/122
摘要： The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.
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公开(公告)号：US07927797B2
公开(公告)日：2011-04-19
申请号：US11045678
申请日：2005-01-28
申请人： John R. Nobile , William L. Lee , John H. Leamon
发明人： John R. Nobile , William L. Lee , John H. Leamon
CPC分类号： B01F3/0807 , B01F5/0413 , B01F5/0471 , B01F13/0059 , B01J2219/00495 , B01J2219/005 , B01J2219/00664 , B01J2219/00722 , B01L3/5027 , B01L7/525 , B01L2200/0673 , B01L2300/1827 , B01L2300/1844 , B01L2400/0439 , B01L2400/0487 , C12Q1/686 , C40B40/06 , C40B60/14 , G01N35/1095 , C12Q2527/15 , C12Q2527/125
摘要： Embodiments of the present invention are directed to methods and devices/systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.
摘要翻译： 本发明的实施方案涉及用于扩增遗传物质的方法和装置/系统，并且可以包括在连续流中提供油包水乳剂。 乳液可以包括多个包含微反应器的水滴。 多个微反应器中的每一个可以包括能够捕获核酸模板，单种核酸模板和用于扩增核酸模板的拷贝数的足够试剂的单个珠。 该方法还包括使乳液流过第一温度区和第二较低温度区，以热处理微反应器以通过聚合酶链反应扩增核酸模板。
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公开(公告)号：US07244567B2
公开(公告)日：2007-07-17
申请号：US10768729
申请日：2004-01-28
申请人： Yi-Ju Chen , John H. Leamon , Kenton L. Lohman , Michael T. Ronan , Jonathan M. Rothberg , Maithreyan Srinivasan , Michael P. Weiner
发明人： Yi-Ju Chen , John H. Leamon , Kenton L. Lohman , Michael T. Ronan , Jonathan M. Rothberg , Maithreyan Srinivasan , Michael P. Weiner
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/5085 , B01L7/52 , B01L2300/0636 , B01L2300/0819 , B01L2300/0877 , C07H21/00 , C12N15/1075 , C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q1/6867 , C12Q1/6869 , C12Q1/6874 , G01N21/253 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N2021/6484 , G01R33/1269 , Y02P20/582 , Y10T436/143333 , C12Q2565/119 , C12Q2531/113 , C12Q2565/537 , C12Q2547/107 , C12Q2547/101 , C12Q2537/143 , C12Q2527/125 , C12Q2525/186 , C12Q2565/301 , C12Q2563/149 , C12Q2531/125 , C12Q2537/149 , C12Q2535/119
摘要： This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.
摘要翻译： 本发明涉及DNA测序方法。 更具体地，本发明涉及通过使用封闭和未封闭的测序引物对DNA的有义链和反义链进行测序的方法。 简而言之，这些方法包括将未封闭引物退火至第一条核酸的步骤; 将第二封闭引物退火至核酸的第二链; 用聚合酶沿着第一链延长核酸; 终止第一个测序引物; 解封第二底漆; 并沿着第二链延长核酸。
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