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公开(公告)号:US5610066A
公开(公告)日:1997-03-11
申请号:US166346
申请日:1993-12-10
申请人: Carl W. Fuller , Joseph Szasz , Frank T. Robb , Kimberly M. Borges , Maria Davis
发明人: Carl W. Fuller , Joseph Szasz , Frank T. Robb , Kimberly M. Borges , Maria Davis
IPC分类号: C07K14/195 , C12N9/00 , C12N9/10 , C12N9/12 , C12N9/22 , C12N9/90 , C12N15/63 , C12N15/11 , C12N15/55
CPC分类号: C12N9/93 , C07K14/195 , C12N9/1077 , C12N9/1252 , C12N9/22 , C12N9/90
摘要: Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS.: 1-8.
摘要翻译: 纯化的核酸,其包含选自SEQ ID NO:1-8的核苷酸碱基序列。
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2.
公开(公告)号:US06875573B2
公开(公告)日:2005-04-05
申请号:US10190967
申请日:2002-07-08
申请人: Carl Fuller , Joseph Szasz
发明人: Carl Fuller , Joseph Szasz
CPC分类号: C12N9/1252
摘要: Thermostable DNA polymerases both in native form and having single amino acid substitutions and optionally N-terminal deletions are disclosed. These polymerases exhibit a substantial improvement of DNA sequencing performance compared to Taq DNA polymerase. The instant DNA polymerases also possess improved salt tolerance.
摘要翻译: 公开了天然形式和具有单个氨基酸取代和任选的N-末端缺失的热稳定的DNA聚合酶。 与Taq DNA聚合酶相比,这些聚合酶显示出DNA测序性能的显着改善。 即时DNA聚合酶也具有改善的耐盐性。
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公开(公告)号:US5939257A
公开(公告)日:1999-08-17
申请号:US738172
申请日:1996-10-25
申请人: Joseph Szasz , Maria Davis
发明人: Joseph Szasz , Maria Davis
CPC分类号: C12N9/16
摘要: Enzymatically active thermostable alkaline phosphatases from Rhodothermus marinus, Thermus thermophilus, and Thermosipho africanus.
摘要翻译: 来自Rhodothermus marinus,Thermus thermophilus和Thermosipho africanus的酶活性热稳定性碱性磷酸酶。
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公开(公告)号:US5633138A
公开(公告)日:1997-05-27
申请号:US452988
申请日:1995-05-30
申请人: Maria Davis , Joseph Szasz
发明人: Maria Davis , Joseph Szasz
CPC分类号: C12N9/16
摘要: Alkaline phosphatase from Thermus thermophilus has been isolated. The enzyme has a pH optimum of greater than 10.5 and is stable to heating at 65.degree. C for 1 hour. The claimed invention relates to a method of detecting nucleic acids in a sample by providing a nucleic acid probe labelled with thermostable alkaline phosphatase from Thermus thermophilus; contacting the sample with said labelled nucleic acid; and detecting said nucleic acid in said sample by means of said thermostable alkaline phosphatase.
摘要翻译: 已经分离了嗜热栖热菌的碱性磷酸酶。 该酶的最适pH值大于10.5,在65℃加热1小时是稳定的。 所要求保护的发明涉及一种通过提供用嗜热嗜热菌耐热碱性磷酸酶标记的核酸探针来检测样品中的核酸的方法; 使样品与所述标记的核酸接触; 以及通过所述耐热碱性磷酸酶检测所述样品中的所述核酸。
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