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1.发明授权Thermostabillization of DNA polymerase by protein folding pathway from a hyperthermophile Archaeon, Pyrococcus furiosus 有权通过蛋白质折叠途径从超嗜热菌（Archaeon），激烈热球菌（Pyrococcus furiosus）中热解DNA聚合酶公开(公告)号：US09416408B2
公开(公告)日：2016-08-16
申请号：US12065152
申请日：2006-08-29
申请人： Frank T. Robb
发明人： Frank T. Robb
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2527/125
摘要： The present invention relates to maintaining the activity and stability of enzymes and biologically active proteins at increased temperatures by contacting same with a combination of isolated passive and active chaperones from a hyperthermopilic Archaeon, wherein the chaperones may include heat shock proteins, prefoldin and/or chaperonin proteins.
摘要翻译： 本发明涉及通过使酶和生物活性蛋白质在高温下保持活性和稳定性，其与来自高热原性考古学的分离的被动和活性伴侣的组合接触，其中伴侣可包括热休克蛋白，预折合蛋白和/或伴侣蛋白 蛋白质。
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2.发明授权Heterologous expression of extremophile heat shock proteins and chaperones in microorganisms to increase tolerance to toxic compounds 有权极微热热休克蛋白和分子伴侣在微生物中的异源表达，以增加对有毒化合物的耐受性公开(公告)号：US08685729B2
公开(公告)日：2014-04-01
申请号：US12528620
申请日：2008-02-26
IPC分类号： C12N5/00 , C12N1/00 , C12N1/20 , C12N15/74 , C12Q1/02 , C12P21/06 , C12P7/06 , C12P7/16 , C07K1/00 , C07H21/04
CPC分类号： C07K14/195 , Y02E50/17
摘要： The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as γ-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.
摘要翻译： 本发明提供了增加微生物对毒性剂如溶剂的耐受性的方法和组合物; 并用于增加溶剂产生微生物的溶剂生产。 所述方法包括将感兴趣的微生物工程化以表达异源热休克蛋白/伴侣，例如II型伴侣蛋白或诸如γ-预折蛋白之类的预折合蛋白，其中异源蛋白质来自极端亲本，例如古细菌。
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3.发明申请公开(公告)号：US20120064560A1
公开(公告)日：2012-03-15
申请号：US12999185
申请日：2009-06-16
申请人： Harold E. Smith , Frank T. Robb
发明人： Harold E. Smith , Frank T. Robb
CPC分类号： C12N15/1086 , C07K14/195
摘要： The present invention relates to recombinant protein production, and more specifically, to methods for recovery of properly folder bioactive proteins by expressing chaperone genes from extremophilic Archaea, during recombinant protein synthesis in a host cell thereby significantly improving recovery of properly folded bioactive proteins.
摘要翻译： 本发明涉及重组蛋白质生产，更具体地说，涉及通过在宿主细胞中重组蛋白质合成期间通过表达来自极细古菌的伴侣蛋白基因来恢复适当的文件夹生物活性蛋白质的方法，从而显着改善正确折叠的生物活性蛋白质的恢复。
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公开(公告)号：US07247432B2
公开(公告)日：2007-07-24
申请号：US10390292
申请日：2003-03-17
IPC分类号： C12Q1/68
CPC分类号： C07K14/195 , C12N9/96 , C12Q1/686 , C12Q2527/125
摘要： Small heat shock proteins, e.g., Pyrococcus fuiosus (Pfu-sHSP and/or Pfu-tsHSP), confer thermotolerance on cellular cultures and on proteins in cellular extracts during prolonged incubation at elevated temperature, demonstrating the ability to protect cellular proteins and maintain cellular viability under heat stress conditions. Such heat shock proteins are effective to combat enzymatic aggregation and intracellular precipitation during heat stress, and thereby enable enhancement of the utility and stability of enzymes in various applications, e.g., Taq polymerase in PCR applications, digestive enzymes in microbial degradative applications, etc.
摘要翻译： 小热休克蛋白，例如热球菌（Pfu-sHSP和/或Pfu-tsHSP），在高温下长时间孵育时赋予细胞培养物上的耐热性和细胞提取物中的蛋白质，证明保护细胞蛋白质并保持细胞活力的能力 在热应力条件下。 这种热休克蛋白在热胁迫期间有效地抵抗酶聚集和细胞内沉淀，从而能够增强酶在各种应用中的效用和稳定性，例如PCR应用中的Taq聚合酶，微生物降解应用中的消化酶等。
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公开(公告)号：US5719056A
公开(公告)日：1998-02-17
申请号：US636928
申请日：1996-04-24
IPC分类号： C12N9/02 , C12N9/04 , C12N9/06 , C12N9/10 , C12N9/22 , C12N9/48 , C12N9/58 , C12N15/31 , C12N15/63 , C12N9/16 , C12N15/11 , C12N15/55
CPC分类号： C12N9/0093 , C12N9/0006 , C12N9/0016 , C12N9/1007 , C12N9/22 , C12N9/48 , C12N9/58
摘要： Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS.: 1-9.
摘要翻译： 纯化的核酸，其包含选自SEQ ID NO：1-9的核苷酸碱基序列。
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6.发明申请HETEROLOGOUS EXPRESSION OF EXTREMOPHILE HEAT SHOCK PROTEINS AND CHAPERONES IN MICROORGANISMS TO INCREASE TOLERANCE TO TOXIC COMPOUNDS 有权微生物中超临界热休克蛋白和葛根酮的异位表达增加对毒性化合物的耐受性公开(公告)号：US20110045554A1
公开(公告)日：2011-02-24
申请号：US12528620
申请日：2008-02-26
IPC分类号： C12P7/64 , C12P1/00 , C12P7/26 , C12P7/16 , C12P7/06 , C12P5/00 , C12P1/04 , C12P1/02 , C12N15/63 , C12N1/00 , C12N1/21 , C12N1/15 , C12N1/19 , C12N1/13 , C12N15/81 , C12N15/74 , C12N15/80 , C12N15/70
CPC分类号： C07K14/195 , Y02E50/17
摘要： The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as γ-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.
摘要翻译： 本发明提供了增加微生物对毒性剂如溶剂的耐受性的方法和组合物; 并用于增加溶剂产生微生物的溶剂生产。 所述方法包括将感兴趣的微生物工程化以表达异源热休克蛋白/伴侣，例如II型伴侣蛋白或诸如γ-预折蛋白之类的预折合蛋白，其中异源蛋白质来自极端亲本，例如古细菌。
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公开(公告)号：US5610066A
公开(公告)日：1997-03-11
申请号：US166346
申请日：1993-12-10
申请人： Carl W. Fuller , Joseph Szasz , Frank T. Robb , Kimberly M. Borges , Maria Davis
发明人： Carl W. Fuller , Joseph Szasz , Frank T. Robb , Kimberly M. Borges , Maria Davis
IPC分类号： C07K14/195 , C12N9/00 , C12N9/10 , C12N9/12 , C12N9/22 , C12N9/90 , C12N15/63 , C12N15/11 , C12N15/55
CPC分类号： C12N9/93 , C07K14/195 , C12N9/1077 , C12N9/1252 , C12N9/22 , C12N9/90
摘要： Purified nucleic acid comprising a nucleotide base sequence selected from sequence ID NOS.: 1-8.
摘要翻译： 纯化的核酸，其包含选自SEQ ID NO：1-8的核苷酸碱基序列。
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8.发明申请Thermostabillization of Dna Polymerase by Protein Folding Pathway from a Hyperthermophile Archaeon, Pyrococcus Furiosus 有权通过蛋白质折叠途径从嗜热菌菌体，热球菌（Pyrococcus furiosus）对Dna聚合酶进行热释放公开(公告)号：US20080318281A1
公开(公告)日：2008-12-25
申请号：US12065152
申请日：2006-08-29
申请人： Frank T. Robb
发明人： Frank T. Robb
CPC分类号： C12Q1/686 , C12Q2527/125
摘要： The present invention relates to maintaining the activity and stability of enzymes and biologically active proteins at increased temperatures by contacting same with a combination of isolated passive and active chaperones from a hyperthermopilic Archaeon, wherein the chaperones may include heat shock proteins, prefoldin and/or chaperonin proteins.
摘要翻译： 本发明涉及通过使酶和生物活性蛋白质在高温下保持活性和稳定性，其与来自高热原性考古学的分离的被动和活性伴侣的组合接触，其中伴侣可包括热休克蛋白，预折合蛋白和/或伴侣蛋白 蛋白质。
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公开(公告)号：US06579703B2
公开(公告)日：2003-06-17
申请号：US09835909
申请日：2001-04-16
IPC分类号： C12D1934
CPC分类号： C12N9/96 , C07K14/195 , C12Q1/686 , C12Q2527/125
摘要： Small heat shock proteins, e.g., Pyrococcus fuiosus (Pfu-sHSP), confer thermotolerance on cellular cultures and on proteins in cellular extracts during prolonged incubation at elevated temperature, demonstrating the ability to protect cellular proteins and maintain cellular viability under heat stress conditions. Such heat shock proteins are effective to combat enzymatic aggregation and intracellular precipitation during heat stress, and thereby enable enhancement of the utility and stability of enzymes in various applications, e.g., Taq polymerase in PCR applications, digestive enzymes in microbial degradative applications, etc.
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