公开(公告)号：US20150377858A1

公开(公告)日：2015-12-31

申请号：US14754567

申请日：2015-06-29

申请人： Gerald Thomas ,  Brian Young ,  Juven Lara ,  Charles Noll ,  James Ingle

发明人： Gerald Thomas ,  Brian Young ,  Juven Lara ,  Charles Noll ,  James Ingle

IPC分类号： G01N33/497

CPC分类号： G01N1/22 ,  A61B5/0071 ,  A61B5/082 ,  A61B5/087 ,  G01D11/24 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/497 ,  G01N33/4972 ,  G01N33/52 ,  G01N33/64 ,  G01N2001/2244 ,  G01N2021/6482 ,  G01N2033/4975 ,  G01N2201/022 ,  G01N2201/062 ,  G01N2201/068 ,  Y10T436/200833 ,  Y10T436/202499

摘要： Fluorescence chromophores such as phenylene diamine derivatives can undergo oxidative coupling and polymerization to form optical, colorimetric and fluorogenic, multimers and polymers. The presence of carbonyl containing moieties such as aldehydes and ketones under favorable environmental conditions can initiate, catalyze, accelerate and modulate this reaction which in turn provides a mechanism for the detection and quantitation of such moieties. Selected phenylene diamine derivatives can be used for the detection and quantitation of aldehyde and ketones via measurement of the reaction and the associated spectroscopic transformation. In particular, the use of meta-phenylene diamine (mPDA) and related compounds for aldehyde detection and quantitation is described. The method provides a convenient means for monitoring aldehyde and ketone levels without use of separation steps. The method is applicable to kinetic and quasi-endpoint detection assay formats.

摘要翻译： 荧光发色团如苯二胺衍生物可进行氧化偶联和聚合以形成光学，比色和荧光，多聚体和聚合物。 在有利的环境条件下，含有羰基的部分如醛和酮的存在可引发，催化，加速和调节该反应，而反应又提供了检测和定量这些部分的机理。 选择的苯二胺衍生物可用于通过测量反应和相关的光谱转化来检测和定量醛和酮。 特别地，描述了使用间苯二胺（mPDA）和相关化合物进行醛检测和定量。 该方法提供了在不使用分离步骤的情况下监测醛和酮水平的方便手段。 该方法适用于动力学和准终点检测测定方法。