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公开(公告)号:US11814639B2
公开(公告)日:2023-11-14
申请号:US16963191
申请日:2019-01-31
CPC分类号: C12N15/8283
摘要: An object of the present invention is to provide a cucumber mosaic virus (CMV)-resistant solanaceous plant. The present invention provides a CMV-resistant solanaceous plant having a mutated eIF4E gene encoding an eIF4E protein nonfunctional for CMV. In addition, the present invention provides a method for producing a CMV-resistant plant, the method comprising mutating an eIF4E gene of a solanaceous plant into a mutated eIF4E gene encoding an eIF4E protein nonfunctional for CMV.
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公开(公告)号:US11766061B2
公开(公告)日:2023-09-26
申请号:US17111911
申请日:2020-12-04
申请人: Kikkoman Corporation
CPC分类号: A23L27/50 , A23L11/50 , A23L27/24 , A23V2002/00
摘要: Provided herein is a salt-free or low-salt soy sauce-like liquid seasoning having a desirable flavor and desirable functionality with no contamination by microorganisms. A method for producing such a sauce-like liquid seasoning is also provided. The salt-free or low-salt soy sauce-like liquid seasoning having a desirable flavor and desirable functionality is obtained by inoculating raw material grains of primarily soybean or wheat with a koji mold to prepare a solid koji, adding salt-free or low-salt water for brewing to the solid koji to prepare a moromi, pasteurizing the moromi, and fermenting the moromi with yeast in a container adapted to reduce entry of harmful microorganisms.
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公开(公告)号:US20230213499A1
公开(公告)日:2023-07-06
申请号:US17801033
申请日:2021-02-18
申请人: KIKKOMAN CORPORATION
发明人: Yosuke MASAKARI , Chiaki INAMOTO , Miki NAKAMURA , Eiichiro KAN , Atsushi ICHIYANAGI , Haruka HIRAGUCHI , Ryota SUZUKI
CPC分类号: G01N33/50 , C12Q1/32 , G01N2333/904 , C12Q2520/00 , C12Q2521/50
摘要: An object of the present invention is to provide a device for evaluating a state of interstitial fluid, blood, urine, tears, sweat, saliva of human and non-human organisms, foods and drinks, brewed product, etc., a system, a program, and an evaluation method using these.
The present invention is to provide a device for evaluating a state of a sample which comprises an action part for allowing lactate dehydrogenase to act on a sample, and a sensor for sensing the state of the sample on which lactate dehydrogenase is allowed to act, a system, a program, a method for evaluating the state of the sample using these, and an enzyme to be used therefor.-
公开(公告)号:US20230203559A1
公开(公告)日:2023-06-29
申请号:US18067158
申请日:2022-12-16
申请人: KIKKOMAN CORPORATION
摘要: A quantitation method of ethanolamine phosphate capable of measuring trace amounts of ethanolamine phosphate, or oxidoreductase used for the quantitation method thereof, a quantitation composition, a quantitation kit, a sensor chip, or a sensor is provided. According to an embodiment of the present invention, a quantitation method of ethanolamine phosphate including allowing phosphatase to act on a sample to convert ethanolamine phosphate contained in the sample into ethanolamine, and allowing oxidoreductase to act on the ethanolamine is provided.
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公开(公告)号:US11629341B2
公开(公告)日:2023-04-18
申请号:US17319362
申请日:2021-05-13
申请人: KIKKOMAN CORPORATION
发明人: Keiichi Ichikawa , Yasutomo Shinohara , Seiichi Hara , Keiko Kurosawa , Yumiko Yamashita , Michiaki Yamashita
摘要: The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine.
(1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).-
公开(公告)号:US11549134B2
公开(公告)日:2023-01-10
申请号:US16832712
申请日:2020-03-27
申请人: Kikkoman Corporation
摘要: Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.
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公开(公告)号:US11518976B2
公开(公告)日:2022-12-06
申请号:US16612103
申请日:2018-05-11
申请人: Kikkoman Corporation
发明人: Tadaomi Kawashima , Naho Ikari , Hiroshi Kawamoto
IPC分类号: C12N1/20 , A23L33/135 , C12N15/74 , A23K10/18
摘要: An object of the present invention is to provide a method for efficiently obtaining a lactic acid bacterium that is made to contain a large amount of double-stranded RNA; and a lactic acid bacterium having a high double-stranded RNA content obtained by the method. The object is achieved by: (1) a method for producing a double-stranded RNA-containing lactic acid bacterium, including a step of culturing a lactic acid bacterium under at least one condition of an aeration condition and a low-temperature condition lower than an optimum temperature, thereby obtaining the double-stranded RNA-containing lactic acid bacterium; (2) a double-stranded RNA-containing lactic acid bacterium, in which the content of double-stranded RNA is 2.0 times or more as compared with the content of double-stranded RNA when a bacterium of the same strain is cultured for the same culture time under an optimum temperature and non-aeration condition; or the like.
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公开(公告)号:US11253567B2
公开(公告)日:2022-02-22
申请号:US16775216
申请日:2020-01-28
申请人: Kikkoman Corporation
发明人: Taro Horiba
IPC分类号: A61K36/752 , A61K31/366 , A61K31/585
摘要: Provided are: a citrus seed extract-containing composition, a food and a drug all of which sufficiently exert a novel function of a citrus (specifically, a citrus seed extract), and a method for producing the citrus seed extract-containing composition. The citrus seed extract-containing composition includes obacunone and nomilin both derived from a citrus seed extract. Herein, the ratio of the obacunone content to the nomilin content (i.e., obacunone content/nomilin content) is at least 0.020.
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公开(公告)号:US20220033840A1
公开(公告)日:2022-02-03
申请号:US17297887
申请日:2019-11-27
发明人: Hiroki ATARASHI , Kenji NAKAHARA
IPC分类号: C12N15/82
摘要: Present invention relates to a virus resistant solanaceous plant, a solanaceous plant cell, and a method for producing the solanaceous plant, and the solanaceous plant has inhibitory properties against: infection by a virus of genus Begomovirus causing tomato yellow leaf curl symptoms, proliferation of the infected virus, and/or expression of infection symptoms. The present invention provides a solanaceous plant having a mutation in at least one gene selected from a group consisting of translation initiation factor eIF4E gene, receptor-like kinase RLK gene, coatomer complex deltaCOP gene, nuclear shuttle protein interactor NSI gene, and genes homologous thereto. The mutation in such a solanaceous plant either inhibits the expression of the mutated gene or makes a protein encoded by the mutated gene to be non-functional for the virus, and the solanaceous plant has virus resistance against the above-mentioned virus.
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公开(公告)号:US11155398B2
公开(公告)日:2021-10-26
申请号:US16500478
申请日:2018-04-02
摘要: To provide a container including an inner container that is deformed as content decreases, the container being a dispensing container reusable and excellent in an aroma retaining property. To implement such container, a dispensing container (10) includes a container body (13) including a flexible inner container configured to be filled with content and to deflate as the content decreases, and an outer container encompassing the inner container and having an intake hole through which external air is taken into a space between the inner container and the outer container, a discharge cap (15) including a top surface part having a discharge port through which the content is discharged, the discharge cap (15) being mounted on a mouth portion (13a) of the container body (13), and an external air introduction hole that provides communication between the outside and the intake hole. The inner container and the outer container are made of a polyethylene terephthalate resin.
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