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7 条记录 (耗时 0.014 秒)

    Human chromosome 16 genes, compositions, methods of making and using same
    3.
    发明授权
    Human chromosome 16 genes, compositions, methods of making and using same 失效
    人染色体16基因，组成，制备和使用方法

    公开(公告)号：US6030806A

    公开(公告)日：2000-02-29

    申请号：US762500

    申请日：1996-12-09

    申请人： Gregory M. Landes ,  Timothy C. Burn ,  Timothy D. Connors ,  William R. Dackowski ,  Terence J. Van Raay ,  Katherine W. Klinger

    发明人： Gregory M. Landes ,  Timothy C. Burn ,  Timothy D. Connors ,  William R. Dackowski ,  Terence J. Van Raay ,  Katherine W. Klinger

    IPC分类号： C07K14/47 ,  C07K14/475 ,  C12P21/06 ,  C07H21/04 ,  C12N5/10 ,  C12P21/02

    CPC分类号： C07K14/475 ,  C07K14/47 ,  A01K2217/05

    摘要： In accordance with the present invention, there are provided isolated nucleic acids encoding a human netrin, a human ATP binding cassette transporter, a human ribosomal L3 subtype, and a human augmenter of liver regeneration as well as isolated protein products encoded thereby. The present invention provides nucleic acid probes that hybridize to invention nucleic acids as well as isolated nucleic acids comprising unique gene sequences located on chromosome 16. Further provided are vectors containing invention nucleic acids, host cells transformed therewith, as well as transgenic non-human mammals that express invention polypeptides. The present invention includes antisense oligonucleotides, antibodies and compositions containing same. Additionally, the invention provides methods for identifying compounds that bind to invention polypeptides.

    摘要翻译： 根据本发明，提供编码人netrin，人ATP结合盒转运蛋白，人核糖体L3亚型和肝再生人增强剂的分离的核酸以及由此编码的分离的蛋白质产物。 本发明提供了与本发明核酸杂交的核酸探针以及包含位于染色体16上的唯一基因序列的分离的核酸。进一步提供了含有本发明核酸，用其转化的宿主细胞以及转基因非人哺乳动物的载体 其表达发明多肽。 本发明包括反义寡核苷酸，抗体和含有它们的组合物。 另外，本发明提供了鉴定结合本发明多肽的化合物的方法。

    Method for obtaining informative cells
    4.
    发明授权
    Method for obtaining informative cells 失效
    获取信息细胞的方法

    公开(公告)号：US5627029A

    公开(公告)日：1997-05-06

    申请号：US243647

    申请日：1994-05-16

    申请人： Timothy W. Houseal ,  Karen Pavelka ,  Katherine W. Klinger ,  William R. Dackowski

    发明人： Timothy W. Houseal ,  Karen Pavelka ,  Katherine W. Klinger ,  William R. Dackowski

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C12N15/00 ,  H01L21/20

    CPC分类号： C12Q1/6841 ,  C12Q1/6806 ,  Y10S435/81 ,  Y10T436/143333

    摘要： The subject invention relates to a method for processing a liquid sample of biological material by: a) transferring a liquid sample containing both uncultured informative cells and non-informative cells onto a substrate wherein the surface tension of the liquid sample is sufficient to maintain the liquid sample beaded on the surface of the substrate; b) incubating the beaded liquid sample on the surface of the substrate for a period of time appropriate for the selective settling of the informative cells onto the surface of the substrate; and c) breaking the surface tension of the liquid portion of the sample wherein the liquid portion of the sample is removed along with the non-informative cells and wherein the informative cells remain on the surface of the substrate.A kit for performing the method is also provided.

    摘要翻译： 本发明涉及通过以下操作来处理生物材料的液体样品的方法：a）将含有未培养的信息细胞和非信息性细胞的液体样品转移到基底上，其中液体样品的表面张力足以维持液体 样品串珠在基底表面上; b）将珠状液体样品在衬底的表面上孵育适合于将信息细胞选择性沉降到衬底的表面上的时间段; 以及c）破坏样品的液体部分的表面张力，其中样品的液体部分与非信息细胞一起被去除，并且其中信息性细胞保留在基底的表面上。 还提供了用于执行该方法的套件。

    Method of detecting cellular material
    5.
    发明授权
    Method of detecting cellular material 失效
    检测细胞材料的方法

    公开(公告)号：US5501954A

    公开(公告)日：1996-03-26

    申请号：US259224

    申请日：1994-06-13

    申请人： Anna M. Mahr ,  Ann E. Bowe ,  Alyson L. Ruff-Roberts ,  Katherine W. Klinger

    发明人： Anna M. Mahr ,  Ann E. Bowe ,  Alyson L. Ruff-Roberts ,  Katherine W. Klinger

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07H21/04 ,  C12Q1/70 ,  G01N33/53

    CPC分类号： C12Q1/6806 ,  C12Q1/6841

    摘要： The method of the present invention relates to a rapid procedure for detecting DNA in a cell, while preserving the morphology of the nucleus for analysis. The method comprises depositing a cell onto a polymeric membrane filter wherein the DNA contained in the cell is retained on the polymeric membrane filter and is available for binding with a fluorescently labeled nucleic acid probe, incubating the polymeric membrane filter with the labeled nulceic acid probe, and detecting the labeled nulceic acid probe wherein detection of the nulceic acid probe is indicative of the presence of the DNA. There are no separate permeabilization steps needed to permit the probes to enter the cell and hybridize to the DNA. The method is simple and quick and is applicable to the sample volumes found in clinical laboratories.

    摘要翻译： 本发明的方法涉及用于检测细胞中DNA的快速方法，同时保留用于分析的细胞核的形态。 该方法包括将细胞沉积在聚合物膜过滤器上，其中细胞中所含的DNA保留在聚合物膜过滤器上，并且可用于与荧光标记的核酸探针结合，将聚合物膜过滤器与标记的硝酸探针， 并检测标记的酸果酸探针，其中检测到硝酸探针指示DNA的存在。 不需要单独的透化步骤来允许探针进入细胞并与DNA杂交。 该方法简便快捷，适用于临床实验室的样品量。

    Polycystic kidney disease gene
    6.
    发明授权
    Polycystic kidney disease gene 失效
    多囊肾病基因

    公开(公告)号：US5654170A

    公开(公告)日：1997-08-05

    申请号：US323443

    申请日：1994-10-12

    申请人： Katherine W. Klinger ,  Gregory M. Landes ,  Timothy C. Burn ,  Timothy D. Connors ,  William Dackowski ,  Gregory Germino ,  Feng Qian

    发明人： Katherine W. Klinger ,  Gregory M. Landes ,  Timothy C. Burn ,  Timothy D. Connors ,  William Dackowski ,  Gregory Germino ,  Feng Qian

    IPC分类号： A61K38/00 ,  C07K14/47 ,  C12N15/12 ,  C12P21/02 ,  C07H21/04 ,  C12N15/63

    CPC分类号： C07K14/47 ,  A61K38/00

    摘要： The present invention involves isolated nucleic acid encoding human PKD1, and sequences derived therefrom. The invention also encompasses vectors comprising these nucleic acids, host cells transformed with the vectors, and methods for producing PKD1 protein or fragments thereof. In another aspect, the invention involves isolated oligonucleotides that hybridize only to the authentic expressed PKD1 gene, and not to PKD1 homologues. In yet another aspect, the invention involves isolated mutant PKD1 genes, and their cDNA cognates. Further provided are isolated oligonucleotides that discriminate between normal and mutant versions of the PKD1 gene. Methods and compositions for treating APKD or disease conditions having the characteristics of APKD are also provided.

    摘要翻译： 本发明涉及编码人PKD1的分离的核酸及其衍生的序列。 本发明还包括包含这些核酸的载体，用载体转化的宿主细胞，以及用于产生PKD1蛋白或其片段的方法。 另一方面，本发明涉及仅与真实表达的PKD1基因杂交的分离的寡核苷酸，而不涉及PKD1同源物。 在另一方面，本发明涉及分离的突变体PKD1基因及其cDNA同源物。 还提供了区分PKD1基因的正常和突变形式的分离的寡核苷酸。 还提供了用于治疗APKD或具有APKD特征的疾病状况的方法和组合物。