公开(公告)号：US20100158924A1

公开(公告)日：2010-06-24

申请号：US12700178

申请日：2010-02-04

申请人： Valerie Clerin ,  Heather H. Shih ,  Kathleen Shields ,  Jeffrey Feldman ,  Gustave T. Hebert ,  Debra D. Pittman ,  Nanhua Dan Deng

发明人： Valerie Clerin ,  Heather H. Shih ,  Kathleen Shields ,  Jeffrey Feldman ,  Gustave T. Hebert ,  Debra D. Pittman ,  Nanhua Dan Deng

IPC分类号： A61K39/395 ,  C07H21/00 ,  C07K14/00 ,  C07K16/00 ,  A61K31/7052 ,  A61K38/02 ,  C12Q1/37 ,  A61P9/00 ,  A61P29/00

CPC分类号： C12N9/6472 ,  A61K38/00 ,  C12Y304/22034 ,  G01N33/5091 ,  G01N2333/95 ,  G01N2800/32

摘要： The present invention provides isolated and purified polynucleotides, polypeptides, and antibodies related to mammalian (e.g., mouse and human) legumain and the novel legumain splice variant, ZB-1. The invention further relates to the use of these isolated and purified polynucleotides, polypeptides, and antibodies, as well as other legumain and ZB-1 agonists and antagonists, in modulating legumain and/or ZB-1 activity, expression, and/or secretion in a cell or cell population, e.g., monocytes, macrophages, foam cells, vascular endothelial cells, kidney proximal tubule cells, arterial endothelial cells, sites of inflammatory cell invasion into a vessel intima, and neointimal lesional areas of an artery. The invention also provides legumain and ZB-1 antagonists, e.g., antagonistic small molecules, antibodies and antibody fragments to legumain and ZB-1, legumain and ZB-1 inhibitory polypeptides, and legumain and ZB-1 inhibitory polynucleotides. The present invention is also directed to novel methods for diagnosing, prognosing, monitoring, treating, ameliorating and/or preventing vascular disorders/diseases and inflammatory disorders/diseases.

摘要翻译： 本发明提供了与哺乳动物（例如，小鼠和人）legumain和新型legumain拼接变体ZB-1相关的分离和纯化的多核苷酸，多肽和抗体。 本发明还涉及这些分离和纯化的多核苷酸，多肽和抗体以及其他legumain和ZB-1激动剂和拮抗剂在调节legumain和/或ZB-1活性，表达和/或分泌中的用途 细胞或细胞群，例如单核细胞，巨噬细胞，泡沫细胞，血管内皮细胞，肾近端小管细胞，动脉内皮细胞，炎性细胞侵入血管内膜的部位和动脉的新内膜损伤区域。 本发明还提供legumain和ZB-1拮抗剂，例如对于legumain和ZB-1，legumain和ZB-1抑制性多肽的拮抗性小分子，抗体和抗体片段，以及legumain和ZB-1抑制性多核苷酸。 本发明还涉及用于诊断，预后，监测，治疗，改善和/或预防血管疾病/疾病和炎症性疾病/疾病的新颖方法。

公开(公告)号：US20070274987A1

公开(公告)日：2007-11-29

申请号：US11806000

申请日：2007-05-25

申请人： Valerie Clerin ,  Heather Shih ,  Kathleen Shields ,  Jeffrey Feldman ,  Gustave Hebert ,  Debra Pittman ,  Nanhua Deng

发明人： Valerie Clerin ,  Heather Shih ,  Kathleen Shields ,  Jeffrey Feldman ,  Gustave Hebert ,  Debra Pittman ,  Nanhua Deng

IPC分类号： A61K39/395 ,  A61K38/17 ,  C07H21/04 ,  A61K39/00 ,  C07K14/705 ,  C07K16/28

CPC分类号： C12N9/6472 ,  A61K38/00 ,  C12Y304/22034 ,  G01N33/5091 ,  G01N2333/95 ,  G01N2800/32

摘要： The present invention provides isolated and purified polynucleotides, polypeptides, and antibodies related to mammalian (e.g., mouse and human) legumain and the novel legumain splice variant, ZB-1. The invention further relates to the use of these isolated and purified polynucleotides, polypeptides, and antibodies, as well as other legumain and ZB-1 agonists and antagonists, in modulating legumain and/or ZB-1 activity, expression, and/or secretion in a cell or cell population, e.g., monocytes, macrophages, foam cells, vascular endothelial cells, kidney proximal tubule cells, arterial endothelial cells, sites of inflammatory cell invasion into a vessel intima, and neointimal lesional areas of an artery. The invention also provides legumain and ZB-1 antagonists, e.g., antagonistic small molecules, antibodies and antibody fragments to legumain and ZB-1, legumain and ZB-1 inhibitory polypeptides, and legumain and ZB-1 inhibitory polynucleotides. The present invention is also directed to novel methods for diagnosing, prognosing, monitoring, treating, ameliorating and/or preventing vascular disorders/diseases and inflammatory disorders/diseases.

摘要翻译： 本发明提供了与哺乳动物（例如，小鼠和人）legumain和新型legumain拼接变体ZB-1相关的分离和纯化的多核苷酸，多肽和抗体。 本发明还涉及这些分离和纯化的多核苷酸，多肽和抗体以及其他legumain和ZB-1激动剂和拮抗剂在调节legumain和/或ZB-1活性，表达和/或分泌中的用途 细胞或细胞群，例如单核细胞，巨噬细胞，泡沫细胞，血管内皮细胞，肾近端小管细胞，动脉内皮细胞，炎性细胞侵入血管内膜的部位和动脉的新内膜损伤区域。 本发明还提供legumain和ZB-1拮抗剂，例如对于legumain和ZB-1，legumain和ZB-1抑制性多肽的拮抗性小分子，抗体和抗体片段，以及legumain和ZB-1抑制性多核苷酸。 本发明还涉及用于诊断，预后，监测，治疗，改善和/或预防血管疾病/疾病和炎症性疾病/疾病的新颖方法。

公开(公告)号：US20050244849A1

公开(公告)日：2005-11-03

申请号：US11014625

申请日：2004-12-16

申请人： Debra Pittman ,  Jeffrey Feldman ,  Kathleen Shields ,  William Trepicchio

发明人： Debra Pittman ,  Jeffrey Feldman ,  Kathleen Shields ,  William Trepicchio

IPC分类号： A61K38/00 ,  C07K14/47 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G06F19/00

CPC分类号： C07K14/4713 ,  A61K38/00

摘要： The invention provides methods for identifying, designing, and optimizing therapeutics for R.A using as targets one or more genes (and/or their encoded gene products) that have been shown to be up- or down-regulated in cells of R.A. relative to normal counterpart cells. Methods and compositions for diagnostic assays for detecting R.A. are also provided.

摘要翻译： 本发明提供了用于鉴定，设计和优化用于作为靶标的治疗的治疗方法，所述基因一个或多个基因（和/或其编码的基因产物）已被证明在R.A.的细胞中被上调或下调。 相对于正常的对应细胞。 用于检测R.A.的诊断测定法的方法和组合物 也提供。