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    Ex vivo remodeling of excised blood vessels for vascular grafts
    3.
    发明申请
    Ex vivo remodeling of excised blood vessels for vascular grafts 审中-公开

    公开(公告)号：US20060155164A1

    公开(公告)日：2006-07-13

    申请号：US11273888

    申请日：2005-11-14

    申请人： Valerie Clerin ,  Rebecca Gusic ,  Keith Gooch

    发明人： Valerie Clerin ,  Rebecca Gusic ,  Keith Gooch

    IPC分类号： A61F2/04

    CPC分类号： A61L27/3695 ,  A61F2/062 ,  A61L27/3625 ,  A61L27/3691 ,  C12N5/0691 ,  Y10S623/916

    摘要： The present invention provides an ex vivo vascular remodeling methods and system by which an excised, small diameter blood vessel can be harvested and expanded to provide viable vascular grafts, as demonstrated at the physical and molecular levels, and as optimized in vivo. The tissue-engineered vessels generated by the present invention closely resemble native vessels in terms of structure, histologically, including endothelial coverage and intricate structural components such as the internal elastic lamina, viability (as measured with the MTT assay and TUNEL analysis), and function (vasoactivity, mechanical and biomechanical properties). Thus, the resulting vascular grafts behave in a manner similar to native arteries in terms of mechanical integrity, and provide clinically relevant patency rates when implanted in vivo. Moreover, the ex vivo methods and system permit the precise control of the mechanical environment involving the excised vessel, while at the same time permitting carefully monitoring of the resulting growth/remodeling, thereby opening new avenues of research regarding the mechanical stimuli responsible for specific aspects of remodeling in vivo.

    Expression of the cysteine protease legumain in vascular and inflammatory diseases
    4.
    发明申请
    Expression of the cysteine protease legumain in vascular and inflammatory diseases 审中-公开
    半胱氨酸蛋白酶在血管和炎症疾病中的表达

    公开(公告)号：US20100158924A1

    公开(公告)日：2010-06-24

    申请号：US12700178

    申请日：2010-02-04

    申请人： Valerie Clerin ,  Heather H. Shih ,  Kathleen Shields ,  Jeffrey Feldman ,  Gustave T. Hebert ,  Debra D. Pittman ,  Nanhua Dan Deng

    发明人： Valerie Clerin ,  Heather H. Shih ,  Kathleen Shields ,  Jeffrey Feldman ,  Gustave T. Hebert ,  Debra D. Pittman ,  Nanhua Dan Deng

    IPC分类号： A61K39/395 ,  C07H21/00 ,  C07K14/00 ,  C07K16/00 ,  A61K31/7052 ,  A61K38/02 ,  C12Q1/37 ,  A61P9/00 ,  A61P29/00

    CPC分类号： C12N9/6472 ,  A61K38/00 ,  C12Y304/22034 ,  G01N33/5091 ,  G01N2333/95 ,  G01N2800/32

    摘要： The present invention provides isolated and purified polynucleotides, polypeptides, and antibodies related to mammalian (e.g., mouse and human) legumain and the novel legumain splice variant, ZB-1. The invention further relates to the use of these isolated and purified polynucleotides, polypeptides, and antibodies, as well as other legumain and ZB-1 agonists and antagonists, in modulating legumain and/or ZB-1 activity, expression, and/or secretion in a cell or cell population, e.g., monocytes, macrophages, foam cells, vascular endothelial cells, kidney proximal tubule cells, arterial endothelial cells, sites of inflammatory cell invasion into a vessel intima, and neointimal lesional areas of an artery. The invention also provides legumain and ZB-1 antagonists, e.g., antagonistic small molecules, antibodies and antibody fragments to legumain and ZB-1, legumain and ZB-1 inhibitory polypeptides, and legumain and ZB-1 inhibitory polynucleotides. The present invention is also directed to novel methods for diagnosing, prognosing, monitoring, treating, ameliorating and/or preventing vascular disorders/diseases and inflammatory disorders/diseases.

    摘要翻译： 本发明提供了与哺乳动物（例如，小鼠和人）legumain和新型legumain拼接变体ZB-1相关的分离和纯化的多核苷酸，多肽和抗体。 本发明还涉及这些分离和纯化的多核苷酸，多肽和抗体以及其他legumain和ZB-1激动剂和拮抗剂在调节legumain和/或ZB-1活性，表达和/或分泌中的用途 细胞或细胞群，例如单核细胞，巨噬细胞，泡沫细胞，血管内皮细胞，肾近端小管细胞，动脉内皮细胞，炎性细胞侵入血管内膜的部位和动脉的新内膜损伤区域。 本发明还提供legumain和ZB-1拮抗剂，例如对于legumain和ZB-1，legumain和ZB-1抑制性多肽的拮抗性小分子，抗体和抗体片段，以及legumain和ZB-1抑制性多核苷酸。 本发明还涉及用于诊断，预后，监测，治疗，改善和/或预防血管疾病/疾病和炎症性疾病/疾病的新颖方法。