公开(公告)号：US20240252668A1

公开(公告)日：2024-08-01

申请号：US18286664

申请日：2022-04-15

申请人： Anne-Sophie BLUEMMEL ,  Kathrin BUNTIN ,  Joseph Anthony D'ALESSIO ,  Liqiong FAN ,  Boris FESSLER ,  Doris GABRIEL ,  Dominik HAINZL ,  Keith HOFFMASTER ,  Shwetha IYER ,  Eshita KHERA ,  Eusebio Manchado ROBLES ,  Pierre MAUDENS ,  Dominik PISTORIUS ,  Roee RAMOT ,  Vincent ROMANET ,  Kuno WUERSCH ,  Padmaja YERRAMILLI-RAO ,  Novartis AG

发明人： Liqiong Fan ,  Dominik Hainzl ,  Roee Ramot ,  Shwetha Iyer ,  Joseph Anthony D'Alessio ,  Kuno Wuersch ,  Dominik Pistorius ,  Eusebio Manchado Robles ,  Kathrin Buntin ,  Doris Gabriel ,  Boris Fessler ,  Pierre Maudens ,  Vincent Romanet ,  Anne-Sophie Bluemmel ,  Keith Hoffmaster ,  Eshita Khera ,  Padmaja Yerramilli-Rao

IPC分类号： A61K47/68 ,  A61P35/00 ,  C07K11/02 ,  C07K14/47 ,  C07K16/28 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/18 ,  C12N15/52 ,  C12P21/02 ,  C12R1/01 ,  G01N33/574

CPC分类号： A61K47/6849 ,  A61K47/6811 ,  A61K47/6865 ,  A61P35/00 ,  C07K11/02 ,  C07K14/4722 ,  C07K16/28 ,  C12N9/0006 ,  C12N9/0073 ,  C12N9/18 ,  C12N9/93 ,  C12N15/52 ,  C12P21/02 ,  C12Y301/01001 ,  G01N33/57492 ,  C12R2001/01

摘要： This application discloses microorganisms and methods of producing GNAQ/GNA11 inhibitors and methods of making antibody drug conjugates of anti-PMEL17 antibodies or antigen binding fragments conjugated to a GNAQ/GNA11 inhibitor. The disclosure also relates to formulations comprising antibody drug conjugates of anti-PMEL 17 antibodies or antigen binding fragments conjugated to a GNAQ/GNA1 inhibitor and methods of treating or preventing cancer using the formulations.

公开(公告)号：US20100035293A1

公开(公告)日：2010-02-11

申请号：US12308138

申请日：2007-06-08

申请人： Kim Brouwer ,  Xianbin Tian ,  Peljin Zhang ,  Keith Hoffmaster

发明人： Kim Brouwer ,  Xianbin Tian ,  Peljin Zhang ,  Keith Hoffmaster

IPC分类号： C12Q1/02 ,  C12N5/07 ,  C12N5/071

CPC分类号： C12N5/067 ,  C12N2500/14 ,  C12N2503/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  G01N33/5014 ,  G01N33/5067

摘要： A method of pulsing cultured hepatocytes, such as sandwich-cultured hepatocytes. The method includes providing a culture of hepatocytes, the culture having at least one bile canaliculus; exposing the culture of hepatocytes to a calcium-free buffer, whereby the contents of the at least one bile canaliculus are released; and removing the calcium-free buffer Pulsing cultured hepatocytes can reduce cholestasis arid can provide an in vitro culture of hepatocytes the more closely reflects in vivo hepatocyte characteristics.

摘要翻译： 脉冲培养的肝细胞如夹心培养的肝细胞的方法。 该方法包括提供肝细胞的培养物，所述培养物具有至少一个胆小管; 将肝细胞培养物暴露于无钙缓冲液中，由此释放至少一个胆小管的内容物; 并去除无钙缓冲液脉冲培养的肝细胞可以减少胆汁淤积，可以提供肝细胞的体外培养，更加体现体内肝细胞特征。