公开(公告)号：US20120064574A1

公开(公告)日：2012-03-15

申请号：US13321038

申请日：2010-05-12

申请人： Ken Tokuyasu ,  Jeung-yil Park ,  Riki Shiroma ,  Muhammad Imran Al-Haq

发明人： Ken Tokuyasu ,  Jeung-yil Park ,  Riki Shiroma ,  Muhammad Imran Al-Haq

IPC分类号： C12P7/14 ,  C07C31/08 ,  C12P3/00 ,  C08B1/00 ,  C01F11/02 ,  C12P19/00

CPC分类号： C12P7/10 ,  C12P19/14 ,  C12P2201/00 ,  D21C1/06 ,  D21C5/005 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/582

摘要： The present invention aims to develop a pretreatment technology for performing efficient saccharification without losing carbohydrates (in particular, free carbohydrates, starch, xylan, or the like) due to solid-liquid separation and washing steps, as a pretreatment for enzymatic saccharification of a lignocellulosic biomass feedstock (including a lignocellulosic biomass feedstock containing readily degradable carbohydrates). Provided are: a production method for a slurry to be used as a substrate for an enzymatic saccharification reaction, comprising: pulverizing an aerial part of a plant as a lignocellulosic biomass feedstock; preparing a slurry containing the biomass feedstock, calcium hydroxide, and water; subjecting the slurry to an alkali treatment; and neutralizing the slurry by introduction of and/or pressurization with carbon dioxide to decrease a pH to 5 to 7; an enzymatic saccharification method, comprising using, as a substrate, a slurry obtained by the production method for a slurry; and a production method for ethanol, comprising using, as a substrate, a saccharification product obtained by the enzymatic saccharification method.

摘要翻译： 本发明旨在开发一种用于进行有效糖化而不会由于固液分离和洗涤步骤而导致的碳水化合物（特别是游离碳水化合物，淀粉，木聚糖等）的预处理技术，作为用于木质纤维素酶促糖化的预处理 生物质原料（包括含有易降解碳水化合物的木质纤维素生物质原料）。 提供：用作用于酶促糖化反应的底物的浆料的制备方法，包括：将植物的地上部分粉碎为木质纤维素生物质原料; 制备含有生物质原料，氢氧化钙和水的浆料; 对浆料进行碱处理; 并通过引入和/或加压二氧化碳来中和浆液以将pH降至5至7; 一种酶促糖化方法，包括使用通过浆料的制备方法获得的浆料作为基材; 以及乙醇的制造方法，其特征在于，使用通过所述酶糖化法得到的糖化物作为底物。

公开(公告)号：US6057144A

公开(公告)日：2000-05-02

申请号：US13067

申请日：1998-01-26

申请人： Ken Tokuyasu ,  Yutaka Mori ,  Shioka Hamamatsu ,  Kiyoshi Hayashi

发明人： Ken Tokuyasu ,  Yutaka Mori ,  Shioka Hamamatsu ,  Kiyoshi Hayashi

IPC分类号： C12N15/09 ,  C08B37/08 ,  C12N1/21 ,  C12N9/78 ,  C12N9/80 ,  C12N15/00 ,  C12N15/55 ,  C12P19/04 ,  C12P19/26 ,  C12R1/00 ,  C12R1/19 ,  C12N15/63

CPC分类号： C12Y305/01041 ,  C08B37/003 ,  C12N9/80 ,  C12P19/04

摘要： A chitin deacetylase gene encoding a protein, a plasmid vector containing said gene and a transformant transformed with the plasmid vector. An object of the present invention is to make contribution to the industrial production of said enzyme, by cloning the gene of said enzyme to elucidate the structure of the gene and express the gene.

摘要翻译： 编码蛋白质的甲壳质脱乙酰酶基因，含有该基因的质粒载体和用质粒载体转化的转化体。 本发明的目的是通过克隆所述酶的基因以阐明基因的结构并表达该基因来对所述酶的工业生产作出贡献。

公开(公告)号：US08759007B2

公开(公告)日：2014-06-24

申请号：US12636434

申请日：2009-12-11

申请人： Sachiko Machida ,  Kiyoshi Hayashi ,  Ken Tokuyasu ,  Yoshikiyo Sakakibara ,  Shigeru Matsunaga

发明人： Sachiko Machida ,  Kiyoshi Hayashi ,  Ken Tokuyasu ,  Yoshikiyo Sakakibara ,  Shigeru Matsunaga

IPC分类号： G01N33/53 ,  C12P21/00 ,  C40B20/08 ,  C40B40/10 ,  C40B50/16

CPC分类号： G01N33/54353 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00725 ,  C40B40/10 ,  G01N33/566

摘要： The present invention provides a method for detecting modified LDL, abnormal cells or bacteria using an intermolecular interaction analysis method, in which a region involved in ligand recognition by a receptor is expressed, without modification or as a biotinylated protein, in cells or in a test tube, and thereafter, the expressed region or the expressed biotinylated protein is immobilized via avidin or streptavidin to a solid phase while the orientation thereof is maintained, and the immobilized protein is utilized; and a kit for detecting the modified LDL or the like.

摘要翻译： 本发明提供了使用分子间相互作用分析方法检测修饰的LDL，异常细胞或细菌的方法，其中在细胞中或在试验中表达受体的配体识别的区域而不经修饰或作为生物素化蛋白质表达 然后，将表达的区域或表达的生物素化蛋白质通过抗生物素蛋白或链霉抗生物素蛋白固定在固相中，同时保持其取向，并利用固定的蛋白质; 以及用于检测修饰的LDL等的试剂盒。

公开(公告)号：US06437107B1

公开(公告)日：2002-08-20

申请号：US09393072

申请日：1999-09-07

申请人： Ken Tokuyasu ,  Yutaka Mori ,  Yuki Kitagawa ,  Kiyoshi Hayashi

发明人： Ken Tokuyasu ,  Yutaka Mori ,  Yuki Kitagawa ,  Kiyoshi Hayashi

IPC分类号： C08B3708

CPC分类号： C07H9/06 ,  C08B37/003

摘要： 2-Methyl-{4-O-(2-amino-2-deoxy-&bgr;-glucopyranosyl)-1,2-dideoxy-&agr;-glucopyrano}(2,1-d)-2-oxazoline of the following formula or an acid addition salt thereof A 50% deacetylated chitin having a repeating structure represented by the following formula, an oligosaccharide thereof or an acid addition salt thereof wherein n is an integer.

摘要翻译： 具有下式的2-甲基 - {4-O-（2-氨基-2-脱氧-β-吡喃葡萄糖基）-1,2-二脱氧-α-吡喃葡萄糖}（2,1-d）-2-恶唑啉或 其酸加成盐具有由下式表示的重复结构的50％脱乙酰壳多糖，其寡糖或其酸加成盐，其中n为整数

公开(公告)号：US5955320A

公开(公告)日：1999-09-21

申请号：US900820

申请日：1997-07-25

申请人： Ken Tokuyasu ,  Hiroshi Ono ,  Mayumi Kameyama ,  Yutaka Mori ,  Shioka Hamamatsu ,  Kiyoshi Hayashi

发明人： Ken Tokuyasu ,  Hiroshi Ono ,  Mayumi Kameyama ,  Yutaka Mori ,  Shioka Hamamatsu ,  Kiyoshi Hayashi

IPC分类号： C07H13/06 ,  C07H15/12 ,  C12P19/26 ,  C07H3/04 ,  C12P19/12

CPC分类号： C07H15/12 ,  C12P19/26

摘要： Di-N-acetyl-D-chitobiose, which is easily available as a chitin decomposate or a chemical reagent, is reacted with a microorganisms-derived deacetylase to produce 2-acetylamino-4-O-(2-amino-2-deoxy-.beta.-D-glucopyranosyl)-2-deoxy-D-glucose of formula (1). ##STR1## The method is quantitative and efficiently produces the compound (1). The compound (1) and its salts are usable as substrates for measuring enzymatic activities, and also as starting substances in the production of various food materials and in the field of glyco-chain engineering.

摘要翻译： 作为几丁质分解物或化学试剂容易获得的二-N-乙酰基-D-壳寡糖与微生物衍生的脱乙酰酶反应以产生2-乙酰氨基-4-O-（2-氨基-2-脱氧 - β-D-吡喃葡萄糖基）-2-脱氧-D-葡萄糖。 该方法是定量和有效地产生化合物（1）。 化合物（1）及其盐可用作测定酶活性的底物，也可用作生产各种食品的起始物质和糖链工程领域。

公开(公告)号：US20100167340A1

公开(公告)日：2010-07-01

申请号：US12636434

申请日：2009-12-11

申请人： Sachiko Machida ,  Kiyoshi Hayashi ,  Ken Tokuyasu ,  Yoshikiyo Sakakibara ,  Shigeru Matsunaga

发明人： Sachiko Machida ,  Kiyoshi Hayashi ,  Ken Tokuyasu ,  Yoshikiyo Sakakibara ,  Shigeru Matsunaga

IPC分类号： C12P21/06

CPC分类号： G01N33/54353 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00725 ,  C40B40/10 ,  G01N33/566

摘要： The present invention provides a method for detecting modified LDL, abnormal cells or bacteria using an intermolecular interaction analysis method, in which a region involved in ligand recognition by a receptor is expressed, without modification or as a biotinylated protein, in cells or in a test tube, and thereafter, the expressed region or the expressed biotinylated protein is immobilized via avidin or streptavidin to a solid phase while the orientation thereof is maintained, and the immobilized protein is utilized; and a kit for detecting the modified LDL or the like.

摘要翻译： 本发明提供了使用分子间相互作用分析方法检测修饰的LDL，异常细胞或细菌的方法，其中在细胞中或在试验中表达受体的配体识别的区域而不经修饰或作为生物素化蛋白质表达 然后，将表达的区域或表达的生物素化蛋白质通过抗生物素蛋白或链霉抗生物素蛋白固定在固相中，同时保持其取向，并利用固定的蛋白质; 以及用于检测修饰的LDL等的试剂盒。

公开(公告)号：US5994520A

公开(公告)日：1999-11-30

申请号：US900819

申请日：1997-07-25

申请人： Ken Tokuyasu ,  Hiroshi Ono ,  Mayumi Kameyama ,  Yutaka Mori ,  Shioka Hamamatsu ,  Kiyoshi Hayashi

发明人： Ken Tokuyasu ,  Hiroshi Ono ,  Mayumi Kameyama ,  Yutaka Mori ,  Shioka Hamamatsu ,  Kiyoshi Hayashi

IPC分类号： C12P19/26 ,  C07H15/203 ,  C07H15/04 ,  C07H15/00 ,  C07H15/10

CPC分类号： C07H15/203

摘要： Disclosed is p-nitrophenyl 2-acetylamino-4-O-(2-amino-2-deoxy-.beta.-D-glucopyranosyl)-2-deoxy-.beta.-D-glucopyranoside of formula (1) and its acid-addition salts. ##STR1## The compound of formula (1) is produced by reacting a p-nitrophenyl derivative of chitin dimer with a microorganism-derived deacetylase. The compound and its salts do not act on exo-type chitinases, and are specific to only endo-type ones.

摘要翻译： 公开了式（1）的对硝基苯基2-乙酰氨基-4-O-（2-氨基-2-脱氧-β-D-吡喃葡萄糖基）-2-脱氧-β-D-吡喃葡萄糖苷及其酸加成盐。 通过使几丁质二聚体的对硝基苯基衍生物与微生物衍生的脱乙酰酶反应来制备式（1）化合物。 该化合物及其盐不作用于外型几丁质酶，仅针对内切型几丁质酶。