公开(公告)号：US20100035325A1

公开(公告)日：2010-02-11

申请号：US12221791

申请日：2008-08-06

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： C12N9/98

CPC分类号： C12N11/04 ,  C12N9/1247 ,  C12N9/1252 ,  C12N9/127 ,  C12N9/1276 ,  C12N9/22 ,  C12N9/93 ,  C12N11/08 ,  C12P19/34

摘要： The present invention discloses thermostable microcapsules comprising a semipermeable membrane, an aqueous core, one or more enzymes in the aqueous core, and a nucleic acid template for an enzyme-mediated reaction in the aqueous core. In one embodiment of the invention, the aqueous core contains one or more polymerases. Microcapsules of the invention can be used in amplification and sequencing reactions. In particular, the microcapsules of the present invention can be used for high-throughput sequencing.

摘要翻译： 本发明公开了包含半透膜，水芯，水芯中的一种或多种酶的热稳定性微胶囊，以及用于在水核中的酶介导的反应的核酸模板。 在本发明的一个实施方案中，含水核心含有一种或多种聚合酶。 本发明的微胶囊可用于扩增和测序反应。 特别地，本发明的微胶囊可用于高通量测序。

公开(公告)号：US20100035323A1

公开(公告)日：2010-02-11

申请号：US12221792

申请日：2008-08-06

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： C12N11/00

CPC分类号： C12N11/08 ,  C12N11/04

摘要： The present invention provides methods for preparing microcapsules, each microcapsule comprising a semipermeable membrane, an aqueous core, one or more enzymes in the aqueous core, and one nucleic acid template in the aqueous core. Microcapsules are prepared by flowing (i) an innermost fluid flow comprising at least one enzyme and at least one nucleic acid template, (ii) a middle fluid flow comprising a semipermeable membrane forming material, and (iii) an outer fluid flow comprising a solution or gas through a multiple sheath flow device. The outer fluid flow entrains the innermost fluid flow and middle fluid flow in the aperture of the multiple sheath flow device. The fluids exit the multiple sheath flow device as a liquid jet resulting in microcapsules.

摘要翻译： 本发明提供了制备微胶囊的方法，每个微胶囊包含半透膜，含水核心，水核心中的一种或多种酶和水核心中的一种核酸模板。 通过流动（i）包含至少一种酶和至少一种核酸模板的最内流体流，（ii）包含半透膜形成材料的中间流体流，和（iii）包含溶液的外部流体流来制备微胶囊 或气体通过多个鞘流装置。 外部流体流动夹带多个鞘流装置的孔中的最内流体流和中流体流。 流体以液体射流离开多个鞘流动装置，产生微胶囊。

公开(公告)号：US20070012113A1

公开(公告)日：2007-01-18

申请号：US11184360

申请日：2005-07-18

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： G01N29/04

CPC分类号： G01N29/225 ,  G01F23/2962 ,  G01N29/222 ,  G01N2291/02836 ,  G01N2291/02854 ,  G01N2291/044 ,  G01N2291/101 ,  G01N2291/102

摘要： Sensors, including ultrasonic components, within a robotic system facilitate the overall operation and efficiency of the robotic system through the detection of objects and surfaces, including the levels of liquids, without contacting the objects or surfaces. The sensors are well-suited for use in connection with microfluidic volumes and biological materials.

摘要翻译： 机器人系统内的传感器（包括超声波部件）通过检测物体和表面（包括液体水平）而不接触物体或表面来促进机器人系统的整体操作和效率。 传感器非常适合与微流体体积和生物材料结合使用。

公开(公告)号：US20110195477A1

公开(公告)日：2011-08-11

申请号：US13057991

申请日：2009-08-06

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： C12N9/98

CPC分类号： C12N11/04 ,  C12N9/1247 ,  C12N9/1252 ,  C12N9/127 ,  C12N9/1276 ,  C12N9/22 ,  C12N9/93 ,  C12N11/08 ,  C12P19/34

摘要： The present invention discloses thermostable microcapsules comprising a semipermeable membrane, an aqueous core, one or more enzymes in the aqueous core, and a nucleic acid template for an enzyme-mediated reaction in the aqueous core. In one embodiment of the invention, the aqueous core contains one or more polymerases. Microcapsules of the invention can be used in amplification and sequencing reactions. In particular, the microcapsules of the present invention can be used for high-throughput sequencing.

摘要翻译： 本发明公开了包含半透膜，水芯，水芯中的一种或多种酶的热稳定性微胶囊，以及用于在水核中的酶介导的反应的核酸模板。 在本发明的一个实施方案中，含水核心含有一种或多种聚合酶。 本发明的微胶囊可用于扩增和测序反应。 特别地，本发明的微胶囊可用于高通量测序。

公开(公告)号：US20080239304A1

公开(公告)日：2008-10-02

申请号：US11865653

申请日：2007-10-01

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： G01N1/10

CPC分类号： B01L3/50273 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0877 ,  B01L2400/049 ,  G01N21/55

摘要： The present invention relates to apparatus, systems, and methods for analyzing biological samples. The apparatus, systems, and methods can involve using a vacuum source to pull microfluidic volumes through analytical equipment, such as flow cells and the like. Additionally, the invention involves using optical equipment in conjunction with the analytical equipment to analyze samples and control the operation thereof.

摘要翻译： 本发明涉及用于分析生物样品的装置，系统和方法。 装置，系统和方法可以涉及使用真空源来通过分析设备（例如流动池等）拉动微流体积。 此外，本发明涉及使用光学设备与分析设备结合来分析样品并控制其操作。

公开(公告)号：US20100091289A1

公开(公告)日：2010-04-15

申请号：US12563677

申请日：2009-09-21

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： G01N21/00

CPC分类号： B01L3/50273 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0877 ,  B01L2400/049 ,  G01N21/55

摘要： The present invention relates to apparatus, systems, and methods for analyzing biological samples. The apparatus, systems, and methods can involve using a vacuum source to pull microfluidic volumes through analytical equipment, such as flow cells and the like. Additionally, the invention involves using optical equipment in conjunction with the analytical equipment to analyze samples and control the operation thereof.

摘要翻译： 本发明涉及用于分析生物样品的装置，系统和方法。 装置，系统和方法可以涉及使用真空源来通过分析设备（例如流动池等）拉动微流体积。 此外，本发明涉及使用光学设备与分析设备结合来分析样品并控制其操作。

公开(公告)号：US07276720B2

公开(公告)日：2007-10-02

申请号：US10990242

申请日：2004-11-16

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： G01N21/85 ,  G01N21/05

CPC分类号： B01L3/50273 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0877 ,  B01L2400/049 ,  G01N21/55

摘要： The present invention relates to apparatus, systems, and methods for analyzing biological samples. The apparatus, systems, and methods can involve using a vacuum source to pull microfluidic volumes through analytical equipment, such as flow cells and the like. Additionally, the invention involves using optical equipment in conjunction with the analytical equipment to analyze samples and control the operation thereof.

摘要翻译： 本发明涉及用于分析生物样品的装置，系统和方法。 装置，系统和方法可以涉及使用真空源来通过分析设备（例如流动池等）拉动微流体积。 此外，本发明涉及使用光学设备与分析设备结合来分析样品并控制其操作。

公开(公告)号：US20060012784A1

公开(公告)日：2006-01-19

申请号：US10990242

申请日：2004-11-16

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： G01N21/00

CPC分类号： B01L3/50273 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0877 ,  B01L2400/049 ,  G01N21/55

摘要： The present invention relates to apparatus, systems, and methods for analyzing biological samples. The apparatus, systems, and methods can involve using a vacuum source to pull microfluidic volumes through analytical equipment, such as flow cells and the like. Additionally, the invention involves using optical equipment in conjunction with the analytical equipment to analyze samples and control the operation thereof.

公开(公告)号：US20110287426A1

公开(公告)日：2011-11-24

申请号：US13113906

申请日：2011-05-23

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/50273 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0877 ,  B01L2400/049 ,  G01N21/55

摘要： The present invention relates to apparatus, systems, and methods for analyzing biological samples. The apparatus, systems, and methods can involve using a vacuum source to pull microfluidic volumes through analytical equipment, such as flow cells and the like. Additionally, the invention involves using optical equipment in conjunction with the analytical equipment to analyze samples and control the operation thereof.

摘要翻译： 本发明涉及用于分析生物样品的装置，系统和方法。 装置，系统和方法可以涉及使用真空源来通过分析设备（例如流动池等）拉动微流体积。 此外，本发明涉及使用光学设备与分析设备结合来分析样品并控制其操作。

公开(公告)号：US20110195474A1

公开(公告)日：2011-08-11

申请号：US13057976

申请日：2009-08-06

申请人： Kevin Ulmer

发明人： Kevin Ulmer

IPC分类号： C12N11/00 ,  B29C39/12

CPC分类号： C12N11/04 ,  C12N11/08

摘要： The present invention provides methods for preparing micro-capsules, each microcapsule comprising a semipermeable membrane, an aqueous core, one or more enzymes in the aqueous core, and one nucleic acid template in the aqueous core. Microcapsules are prepared by flowing (i) an innermost fluid flow comprising at least one enzyme and at least one nucleic acid template, (ii) a middle fluid flow comprising a semipermeable membrane forming material, and (iii) an outer fluid flow comprising a solution or gas through a multiple sheath flow device. The outer fluid flow entrains the innermost fluid flow and middle fluid flow in the aperture of the multiple sheath flow device. The fluids exit the multiple sheath flow device as a liquid jet resulting in microcapsules.

摘要翻译： 本发明提供了制备微胶囊的方法，每个微胶囊包含半透膜，水芯，含水芯中的一种或多种酶和水核心中的一种核酸模板。 通过流动（i）包含至少一种酶和至少一种核酸模板的最内流体流，（ii）包含半透膜形成材料的中间流体流，和（iii）包含溶液的外部流体流来制备微胶囊 或气体通过多个鞘流装置。 外部流体流动夹带多个鞘流装置的孔中的最内流体流和中流体流。 流体以液体射流离开多个鞘流动装置，产生微胶囊。