利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.012 秒)

    Methods for protein purification using aqueous two-phase extraction
    3.
    发明授权
    Methods for protein purification using aqueous two-phase extraction 有权
    使用水相两相萃取的蛋白质纯化方法

    公开(公告)号：US06437101B1

    公开(公告)日：2002-08-20

    申请号：US09307549

    申请日：1999-05-07

    申请人： Kirk James Hayenga ,  Pascal P. Valex

    发明人： Kirk James Hayenga ,  Pascal P. Valex

    IPC分类号： A61K3824

    CPC分类号： C07K14/61

    摘要： Methods are provided in this invention for the isolation of human growth hormone, growth hormone antagonist, or a homologue of either, from a biological source. The methods of the invention use multi-phase extraction.

    摘要翻译： 在本发明中提供了用于从生物来源分离人生长激素，生长激素拮抗剂或其同源物的方法。 本发明的方法使用多相萃取。

    Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
    4.
    发明授权
    Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same 有权
    在丝状真菌中表达的异源多肽，其制备方法和用于制备其的载体

    公开(公告)号：US06379928B1

    公开(公告)日：2002-04-30

    申请号：US09468265

    申请日：1999-12-10

    申请人： Randy Michael Berka ,  Daniel Cullen ,  Gregory Lawrence Gray ,  Kirk James Hayenga ,  Virgil Bryan Lawlis

    发明人： Randy Michael Berka ,  Daniel Cullen ,  Gregory Lawrence Gray ,  Kirk James Hayenga ,  Virgil Bryan Lawlis

    IPC分类号： C12P2100

    CPC分类号： C12N9/2428 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/61 ,  C12N9/58 ,  C12N9/6481 ,  C12N15/62 ,  C12N15/80

    摘要： Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3′ terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.

    摘要翻译： 公开了用于从丝状真菌表达和分泌异源多肽的新型载体。 这些载体用于表达和分泌这些异源多肽的新方法中。 用于转化丝状真菌以表达和分泌异源多肽的载体包括编码异源多肽的DNA序列和编码在给定丝状真菌中在分泌系统中有功能的信号序列的DNA序列，并且其与 编码异源多肽的序列。 这样的信号序列可以是通常与异源多肽相关的信号序列，或者可以来自其他来源。 载体还可以含有编码启动子序列的DNA序列，其由丝状真菌功能识别，并且可操作地连接于编码信号序列的DNA序列。 优选地，功能性多腺苷酸化序列可操作地连接到编码异源多肽的DNA序列的3'末端。 上述载体中的每一种用于转化丝状真菌的新方法，其中表达编码信号序列和异源多肽的DNA序列。 由此合成的多肽此后由丝状真菌分泌。

    Heterologous polypeptides expressed in filamentous fungi, process for making same, and vectors for making same
    5.
    发明授权
    Heterologous polypeptides expressed in filamentous fungi, process for making same, and vectors for making same 失效
    在丝状真菌中表达的异源多肽，其制备方法和用于制备其的载体

    公开(公告)号：US06171817B2

    公开(公告)日：2001-01-09

    申请号：US08976967

    申请日：1997-11-24

    申请人： Randy Michael Berka ,  Daniel Cullen ,  Gregory Lawrence Gray ,  Kirk James Hayenga ,  Virgil Bryan Lawlis

    发明人： Randy Michael Berka ,  Daniel Cullen ,  Gregory Lawrence Gray ,  Kirk James Hayenga ,  Virgil Bryan Lawlis

    IPC分类号： C12N1500

    CPC分类号： C12N9/2428 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/61 ,  C12N9/58 ,  C12N9/6481 ,  C12N15/62 ,  C12N15/80

    摘要： Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3′ terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.

    摘要翻译： 公开了用于从丝状真菌表达和分泌异源多肽的新型载体。 这些载体用于表达和分泌这些异源多肽的新方法中。 用于转化丝状真菌以表达和分泌异源多肽的载体包括编码异源多肽的DNA序列和编码在给定丝状真菌中在分泌系统中有功能的信号序列的DNA序列，并且可操作地连接到 编码异源多肽的序列。 这样的信号序列可以是通常与异源多肽相关的信号序列，或者可以来自其他来源。 载体还可以含有编码启动子序列的DNA序列，其由丝状真菌功能识别，并且可操作地连接于编码信号序列的DNA序列。 优选地，功能性多腺苷酸化序列可操作地连接到编码异源多肽的DNA序列的3'末端。 上述载体中的每一种用于转化丝状真菌的新方法，其中表达编码信号序列和异源多肽的DNA序列。 由此合成的多肽此后由丝状真菌分泌。