公开(公告)号：US5064541A

公开(公告)日：1991-11-12

申请号：US499864

申请日：1990-03-27

Applicant: Tzyy-Wen Jeng ,  Kristin D. Elmore ,  Gary M. Oosta ,  Terry A. Pry

Inventor： Tzyy-Wen Jeng ,  Kristin D. Elmore ,  Gary M. Oosta ,  Terry A. Pry

IPC: B01D39/00 ,  B01D39/16 ,  B01D39/18 ,  B01D39/20 ,  B01L3/00 ,  G01N33/49 ,  G01N33/52 ,  G01N33/53

CPC classification number: B01L3/508 ,  B01D39/00 ,  B01D39/163 ,  B01D39/1661 ,  B01D39/18 ,  B01D39/201 ,  B01D39/2034 ,  B01D39/2075 ,  B01L3/5023 ,  G01N33/491 ,  G01N33/526 ,  G01N33/5302 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0825 ,  B01L2300/087 ,  B01L2400/0406 ,  Y10T436/25375

Abstract: A device and method for permitting the separation of plasma or serum from whole blood. The device comprises a matrix of hydrophilic sintered porous material to which at least one red blood cell agglutinating agent has been applied. According to a first method of using the device, a sample of whole blood is applied to a first end of the matrix and the red blood cells within the sample come in contact with the agglutinating agents present in the matrix. The blood cells agglutinate, and are entrapped in the interstices of the matrix, while substantially blood-cell-free serum or plasma accumulates near the outlet of the device. A filter means in liquid receiving relationship with the matrix functions to wick the serum of plasma from the matrix.According to an alternative aspect of the invention, a filter means in liquid receiving relationship with the outlet of the matrix functions to retain any blood cells which pass through the matrix as the filter means wicks the plasma or serum from the matrix. Additional agglutinating agents may be incorporated within the filter means to facilitate retention of blood cells which pass through the matrix.Another aspect of the present invention involves a device for the measurement of a serum or plasma sample, utilizing a matrix of sintered porous material, such as sintered glass, sintered steel, sintered ceramics, sintered plastics, and equivalents thereof. The matrix is characterized by a reproducible fluid uptake capacity proportional to the fixed dimensions of said matrix, a minimal reactivity with plasma or serum components, and a hydrophilic internal surface which enables the matrix to collect and retain a predetermined volume of sample for analysis.

Abstract translation: 允许血浆或血清从全血分离的装置和方法。 该装置包括已经施加至少一种红细胞凝集剂的亲水性烧结多孔材料的基质。 根据使用该装置的第一种方法，将全血样品施加到基质的第一端，并且样品内的红细胞与存在于基质中的凝集剂接触。 血细胞凝集，并被截留在基质的空隙中，而基本上无血细胞的血清或血浆聚集在装置的出口附近。 与基质液体接收关系的过滤装置用于从基质中吸收血浆的血清。 根据本发明的另一方面，与基质的出口液体接收关系的过滤器装置用于保持通过基质的任何血细胞，因为过滤器装置将血浆或血清从基质中吸出。 可以在过滤器装置内并入额外的凝集剂以促进通过基质的血细胞的保留。 本发明的另一方面涉及一种利用烧结多孔材料如烧结玻璃，烧结钢，烧结陶瓷，烧结塑料及其等同物的基质来测量血清或血浆样品的装置。 该基质的特征在于与所述基质的固定尺寸成比例的可再现的流体吸收能力，与血浆或血清组分的最小反应性，以及亲水性内表面，其使基质收集并保留预定体积的样品用于分析。