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    SOLUBLE EXPRESSION OF BULKY FOLDED ACTIVE PROTEINS
    3.
    发明申请
    SOLUBLE EXPRESSION OF BULKY FOLDED ACTIVE PROTEINS 审中-公开
    大肠杆菌折叠活性蛋白的可溶性表达

    公开(公告)号：US20130084602A1

    公开(公告)日：2013-04-04

    申请号：US13643137

    申请日：2011-03-03

    申请人： Sang Jun Lee ,  Young Ok Kim ,  Bo Hye Nam ,  Hee Jeong Kong ,  Kyung Kil Kim

    发明人： Sang Jun Lee ,  Young Ok Kim ,  Bo Hye Nam ,  Hee Jeong Kong ,  Kyung Kil Kim

    IPC分类号： C12N15/70 ,  C12P21/06

    CPC分类号： C12N15/625 ,  C07K2319/02 ,  C07K2319/50 ,  C12N15/70 ,  C12P21/06

    摘要： The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.

    摘要翻译： 本发明涉及通过将前导肽与酸性或碱性pI连接来增强异源蛋白质，特别是具有一个或多个跨膜样结构域或分子内二硫键的大体积折叠活性异源蛋白质的可溶性表达和分泌的表达载体和方法 并具有高亲水性; 通过用具有酸性或中性pI和高亲水性的异源蛋白的N-末端取代一个或多个氨基酸; 或降低编码具有碱性pI值和高亲水性的前导肽的多核苷酸的升高GRNA值。 表达载体和方法可以用于产生异源蛋白质并通过防止不溶性包涵体的形成和通过增强异源蛋白质分泌到周质或外部细胞中的分泌效率来转导患者中的治疗性蛋白质。

    PRODUCTION OF SOLUBLE RECOMBINANT PROTEIN BY PI VALUE CONTROL OF N-TERMINAL
    4.
    发明申请
    PRODUCTION OF SOLUBLE RECOMBINANT PROTEIN BY PI VALUE CONTROL OF N-TERMINAL 审中-公开
    通过PI端控制N-末端生产可溶性重组蛋白

    公开(公告)号：US20100305040A1

    公开(公告)日：2010-12-02

    申请号：US12745187

    申请日：2008-04-17

    申请人： Sang Jun Lee ,  Young Ok Kim ,  Bo Hye Nam

    发明人： Sang Jun Lee ,  Young Ok Kim ,  Bo Hye Nam

    IPC分类号： A61K38/02 ,  C12N15/63 ,  C12N5/10 ,  C12P21/00 ,  C12Q1/02 ,  C07K19/00 ,  C12P21/06 ,  A61P25/00

    CPC分类号： C12N15/62 ,  C12N15/625

    摘要： The present invention relates to a method for improving secretion efficiency of a recombinant foreign protein using a polypeptide fragment containing N-region of a signal sequence (directional signal) or variants thereof with the controlled pI value and/or a secretional enhancer composed of a hydrophilic polypeptide with the controlled pI value. The method of the present invention can be not only useful for the production of a recombinant foreign protein by preventing precipitation of an insoluble precipitate and by increasing extracellular or extra-periplasmic secretion efficiency of a recombinant protein but also useful for the transduction of an effective therapeutic protein by increasing membrane permeability using a strong secretional enhancer.

    摘要翻译： 本发明涉及使用含有受控pI值的信号序列（定向信号）或其变体的N区的多肽片段和/或由亲水性组成的分泌增强子来改善重组外源蛋白的分泌效率的方法 具有受控pI值的多肽。 本发明的方法不仅可用于通过防止不溶性沉淀物的沉淀和通过增加重组蛋白的细胞外或超周质分泌效率来生产重组外源蛋白，而且可用于转导有效治疗 蛋白质通过使用强分泌增强剂增加膜通透性。

    PRODUCTION OF A SOLUBLE NATIVE FORM OF RECOMBINANT PROTEIN BY THE SIGNAL SEQUENCE AND SECRETIONAL ENHANCER
    5.
    发明申请
    PRODUCTION OF A SOLUBLE NATIVE FORM OF RECOMBINANT PROTEIN BY THE SIGNAL SEQUENCE AND SECRETIONAL ENHANCER 审中-公开
    通过信号序列和分泌增强剂生产重组蛋白的可溶性本体

    公开(公告)号：US20090011995A1

    公开(公告)日：2009-01-08

    申请号：US12162118

    申请日：2007-01-30

    申请人： Sang Jun Lee ,  Young Ok Kim ,  Bo-Hye Nam

    发明人： Sang Jun Lee ,  Young Ok Kim ,  Bo-Hye Nam

    IPC分类号： A61K38/16 ,  C12N15/64 ,  C12N1/21 ,  C12P21/00 ,  C12N5/10 ,  A61P25/00 ,  C12N1/19 ,  C12Q1/68 ,  C07K14/00

    CPC分类号： C12N15/63 ,  C07K14/43509 ,  C07K14/461 ,  C07K2319/02 ,  C07K2319/034 ,  C07K2319/50 ,  C12P21/02

    摘要： The present invention is drawn to a method for enhancing secretional efficiency of a heterologous protein using a secretional enhancer consisting of a modified signal sequence which comprises the N-region of a signal sequence and/or a hydrophobic fragment of the said signal sequence comprising the said N-region and/or the hydrophilic polypeptide. The method of the present invention can be used not only for production of recombinant heterologous proteins by inhibiting insoluble precipitation and enhancing secretional efficiency of the recombinant protein into the periplasm or the extracellular fluid and but also for transduction of therapeutic proteins by enhancing membrane-permeability of the recombinant protein using a strong secretional enhancer.

    摘要翻译： 本发明涉及一种用于增强异源蛋白质的分泌效率的方法，该方法使用由修饰的信号序列组成的分泌增强子，所述修饰的信号序列包含所述信号序列的N区和/或所述信号序列的疏水性片段，所述信号序列包含所述 N-区和/或亲水性多肽。 本发明的方法不仅可用于通过抑制不溶性沉淀和提高重组蛋白进入周质或细胞外液的分泌效率而且通过增强膜的渗透性来转导治疗性蛋白质来生产重组异源蛋白 该重组蛋白使用强分泌增强子。

    Phytase produced from Citrobacter braakii
    6.
    发明授权
    Phytase produced from Citrobacter braakii 有权
    柠檬酸杆菌生产的植酸酶

    公开(公告)号：US07442398B2

    公开(公告)日：2008-10-28

    申请号：US10550758

    申请日：2004-03-25

    申请人： Young Ok Kim ,  Han Woo Kim ,  Jeong Ho Lee ,  Kyung Kil Kim ,  Jong Yun Lee ,  In Soo Kong

    发明人： Young Ok Kim ,  Han Woo Kim ,  Jeong Ho Lee ,  Kyung Kil Kim ,  Jong Yun Lee ,  In Soo Kong

    IPC分类号： A23L1/36 ,  C12N9/16 ,  C12P19/30 ,  C12P21/06 ,  C07H21/02 ,  C07H21/04 ,  A23C9/121

    CPC分类号： C12N9/16 ,  A23K20/189

    摘要： The present invention relates to phytase enzyme, a gene encoding the enzyme, and a Citrobacter sp. producing the enzyme. Particularly, the present invention relates to the phytase enzyme produced from Citrobacter sp. having (a) molecular weight of 47 kDa, (b) optimal pH of 3.5-4.5, (c) optimal temperature of 45-55° C., (d) a substrates phytate, p-nitrophenyl phosphate, tetrasodium pyrophosphate, ATP or ADP, (e) Michaelis constant of 0.3-0.5 mM utilizing phytate as substrate, and (f) high resistance to protease such as pepsin, trypsin, papain, elastase or pancreatin. The present invention also relates to the gene coding the phytase enzyme and the Citrobacter braakii producing the enzyme. The phytase enzyme and the Citrobacter braakii producing the enzyme of the present invention can be used in manufacturing a feed of monogastrics as feed additive and in recovering a specific decomposition product of phytate at low price.

    摘要翻译： 本发明涉及植酸酶，编码酶的基因和柠檬酸杆菌属。 产生酶。 特别地，本发明涉及由柠檬酸杆菌属（Citrobacter sp。）生产的植酸酶。 （a）分子量为47kDa，（b）最佳pH为3.5-4.5，（c）最适温度为45-55℃，（d）底物植酸盐，磷酸对硝基苯酯，焦磷酸四钠，ATP或 ADP，（e）利用植酸盐作为底物的0.3-0.5mM的米氏常数，和（f）对蛋白酶如胃蛋白酶，胰蛋白酶，木瓜蛋白酶，弹性蛋白酶或胰酶的高抗性。 本发明还涉及编码植酸酶的基因和产生该酶的柠檬酸杆菌（Citrobacter braakii）。 产生本发明酶的植酸酶和柠檬酸杆菌可用于制造作为饲料添加剂的单胃饲料的饲料，并以低价回收植酸盐的特定分解产物。

    Liquid crystal display device and method for driving the same
    8.
    发明授权
    Liquid crystal display device and method for driving the same 有权
    液晶显示装置及其驱动方法

    公开(公告)号：US07834831B2

    公开(公告)日：2010-11-16

    申请号：US11411904

    申请日：2006-04-27

    申请人： Young Ok Kim

    发明人： Young Ok Kim

    IPC分类号： G09G3/36 ,  G02F1/1345

    CPC分类号： G02F1/13452 ,  G02F1/1345

    摘要： A COG type LCD device and method are disclosed, which improve picture quality by minimizing driving voltage drop. The device according to an embodiment comprises an image area including a thin film transistor and a pixel electrode in a pixel region defined by gate and data lines crossing each other. A plurality of drive IC chips are formed using a COG (Chip On Glass) method in the periphery of the image display area. A LOG (Line On Glass) line is provided for cascading the plurality of drive IC chips to divide various signals inputted from an external driving circuit and to provide the divided signals to the respective drive IC chips. The LOG line includes analog and digital power lines. First and second switches are provided between the analog and digital power lines so as to temporarily short the analog and digital power lines.

    摘要翻译： 公开了一种COG型LCD装置和方法，其通过最小化驱动电压降来提高图像质量。 根据实施例的器件包括由彼此交叉的栅极和数据线限定的像素区域中的薄膜晶体管和像素电极的图像区域。 在图像显示区域的周围使用COG（玻璃上芯片）方法形成多个驱动IC芯片。 提供LOG（Line On Glass）线，用于级联多个驱动IC芯片以分割从外部驱动电路输入的各种信号，并将分频信号提供给相应的驱动IC芯片。 LOG线包括模拟和数字电源线。 第一和第二开关设置在模拟和数字电源线之间，以便暂时缩短模拟和数字电力线。