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6 条记录 (耗时 0.018 秒)

    Location Analysis Using Fire Retardant-Protected Nucleic Acid-Labeled Tags
    1.
    发明申请
    Location Analysis Using Fire Retardant-Protected Nucleic Acid-Labeled Tags 有权
    使用阻燃保护的核酸标记标签的位置分析

    公开(公告)号：US20110306141A1

    公开(公告)日：2011-12-15

    申请号：US12815981

    申请日：2010-06-15

    申请人： Mary F. Bronchetti ,  Laura T. Bortolin

    发明人： Mary F. Bronchetti ,  Laura T. Bortolin

    IPC分类号： G09F3/00 ,  C09K21/00

    CPC分类号： C12Q1/68 ,  C12Q1/6816 ,  Y10T436/13 ,  C12Q2565/514

    摘要： A nucleic acid tag comprising a nucleotide-support platform attached to a nucleic acid molecule, a fire retardant, and an encapsulant. Unique nucleic acid-containing tags containing a fire- or heat-protective element are seeded at one or more geographic locations. Using sequence analysis techniques, the person or object of interest is examined for the presence of one or more of the seeded nucleic acids. The geographic location associated with each detected nucleic acid is used to backtrack the item's path or extrapolate a probable point of origin.

    摘要翻译： 包含与核酸分子连接的核苷酸支持平台，阻燃剂和密封剂的核酸标签。 含有耐火或热保护元件的独特的含有核酸的标签种植在一个或多个地理位置。 使用序列分析技术，检查感兴趣的人或物体是否存在一种或多种接种的核酸。 与每个检测到的核酸相关联的地理位置用于回溯物品的路径或外推可能的原点。

    PNA-DNA oligomers and methods of use thereof
    4.
    发明申请
    PNA-DNA oligomers and methods of use thereof 审中-公开
    PNA-DNA寡聚体及其使用方法

    公开(公告)号：US20080131880A1

    公开(公告)日：2008-06-05

    申请号：US11604014

    申请日：2006-11-22

    申请人： Laura T. Bortolin ,  Christina M. Rudzinski ,  Amanda L. Stephens

    发明人： Laura T. Bortolin ,  Christina M. Rudzinski ,  Amanda L. Stephens

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34 ,  G06G7/48

    CPC分类号： C12Q1/6823 ,  C12Q2525/107 ,  C12Q2525/197 ,  C12Q2565/30 ,  C12Q2521/319

    摘要： Peptide nucleic acid (PNA)-deoxyribonucleic acid (DNA) oligomers and methods of using the PNA-DNA oligomers to detect and/or amplify a target nucleic acid in a sample are provided. The PNA-DNA oligomers of the invention are relatively insensitive to ionic concentration and many inhibitory proteins and, consequently, are particularly advantageous for direct use in environmental or challenging samples to detect nucleic acid, especially that of microorganisms, including food and water pathogens and/or bioterrorism agents. Methods are also provided for use of the PNA-DNA oligomers in applications including polymerase chain reaction, nucleic acid sequencing, mutation detection and as nucleic acid probes.

    摘要翻译： 提供了肽核酸（PNA） - 脱氧核糖核酸（DNA）寡聚体和使用PNA-DNA寡聚体检测和/或扩增样品中靶核酸的方法。 本发明的PNA-DNA寡聚体对离子浓度和许多抑制性蛋白质相对不敏感，因此特别有利于直接用于环境或挑战样品中以检测核酸，特别是微生物，包括食物和水病原体和/ 或生物恐怖主义者。 还提供了在应用中使用PNA-DNA寡聚体的方法，包括聚合酶链反应，核酸测序，突变检测和核酸探针。

    Apparatus and method for isolating a nucleic acid from a sample
    5.
    发明授权
    Apparatus and method for isolating a nucleic acid from a sample 失效
    从样品中分离核酸的装置和方法

    公开(公告)号：US07217513B2

    公开(公告)日：2007-05-15

    申请号：US10193742

    申请日：2002-07-10

    申请人： Lalitha Parameswaran ,  Albert Young ,  Laura T. Bortolin ,  Mark A. Hollis ,  James Harper ,  Johanna Bobrow

    发明人： Lalitha Parameswaran ,  Albert Young ,  Laura T. Bortolin ,  Mark A. Hollis ,  James Harper ,  Johanna Bobrow

    IPC分类号： C12Q1/68

    CPC分类号： B01L3/0282 ,  B01L3/0241 ,  B01L3/5023 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/049 ,  C12Q1/6806 ,  C12Q2565/137 ,  C12Q2549/125

    摘要： A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The method can be conducted in an apparatus that includes a porous support having a component that deactivates a nucleic acid amplification inhibitor component of a sample contacting the porous support and a housing having an opening and defining an interior, said interior being in fluid communication with the porous support, whereby at least a portion of a fluid directed through the opening is directed through at least a portion of the porous support and separates at least a portion of a nucleic acid component of a sample contacting the porous support from the support, thereby preparing the nucleic acid component for amplification.

    摘要翻译： 用于制备用于扩增的样品的核酸组分的方法包括使样品与多孔载体接触，多孔载体使样品的核酸扩增抑制剂组分失活并引导流体通过多孔载体，由此样品的核酸组分为 引导通过多孔载体的至少一部分并与载体分离，由此制备用于扩增的核酸组分。 该方法可以在包括多孔载体的装置中进行，所述多孔载体具有使与多孔载体接触的样品的核酸扩增抑制剂组分失活的组分和具有开口并限定内部的壳体，所述内部与 多孔载体，其中通过开口引导的流体的至少一部分被引导通过多孔载体的至少一部分，并将与多孔载体接触的样品的核酸组分的至少一部分与载体分离，由此制备 用于扩增的核酸组分。

    Location analysis using fire retardant-protected nucleic acid-labeled tags
    6.
    发明授权
    Location analysis using fire retardant-protected nucleic acid-labeled tags 有权
    使用阻燃保护核酸标签的位置分析

    公开(公告)号：US08703493B2

    公开(公告)日：2014-04-22

    申请号：US12815981

    申请日：2010-06-15

    申请人： Mary F. Bronchetti ,  Laura T. Bortolin

    发明人： Mary F. Bronchetti ,  Laura T. Bortolin

    IPC分类号： G01N37/00

    CPC分类号： C12Q1/68 ,  C12Q1/6816 ,  Y10T436/13 ,  C12Q2565/514

    摘要： A nucleic acid tag comprising a nucleotide-support platform attached to a nucleic acid molecule, a fire retardant, and an encapsulant. Unique nucleic acid-containing tags containing a fire- or heat-protective element are seeded at one or more geographic locations. Using sequence analysis techniques, the person or object of interest is examined for the presence of one or more of the seeded nucleic acids. The geographic location associated with each detected nucleic acid is used to backtrack the item's path or extrapolate a probable point of origin.

    摘要翻译： 包含与核酸分子连接的核苷酸支持平台，阻燃剂和密封剂的核酸标签。 含有耐火或热保护元件的独特的含有核酸的标签种植在一个或多个地理位置。 使用序列分析技术，检查感兴趣的人或物体是否存在一种或多种接种的核酸。 与每个检测到的核酸相关联的地理位置用于回溯物品的路径或外推可能的原点。