公开(公告)号：US20080131949A1

公开(公告)日：2008-06-05

申请号：US11804598

申请日：2007-05-18

申请人： Laura T. Bortolin ,  Lalitha Parameswaran ,  James Harper ,  Johanna Bobrow ,  Mark A. Hollis ,  Drew Chapman Brown ,  Eric Scott Clasen ,  John Calvin Schmidt

发明人： Laura T. Bortolin ,  Lalitha Parameswaran ,  James Harper ,  Johanna Bobrow ,  Mark A. Hollis ,  Drew Chapman Brown ,  Eric Scott Clasen ,  John Calvin Schmidt

IPC分类号： C12N13/00 ,  C12M1/00

CPC分类号： B01L3/502 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/043 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/048 ,  B01L2300/0609 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L2400/0644 ,  B01L2400/0683 ,  C07H21/04 ,  C12N15/1013

摘要： A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The sample is separated from raw sample components through means that include a magnetic substrate. An apparatus suitable for conducting the method of the invention also is described.

摘要翻译： 用于制备用于扩增的样品的核酸组分的方法包括使样品与多孔载体接触，多孔载体使样品的核酸扩增抑制剂组分失活并引导流体通过多孔载体，由此样品的核酸组分为 引导通过多孔载体的至少一部分并与载体分离，由此制备用于扩增的核酸组分。 样品通过包括磁性底物的方式与原始样品组分分离。 还描述了适用于进行本发明的方法的装置。

公开(公告)号：US08062846B2

公开(公告)日：2011-11-22

申请号：US11804598

申请日：2007-05-18

申请人： Laura T. Bortolin ,  Lalitha Parameswaran ,  James Harper ,  Johanna Bobrow ,  Mark A. Hollis ,  Drew Chapman Brown ,  Eric Scott Clasen ,  John Calvin Schmidt

发明人： Laura T. Bortolin ,  Lalitha Parameswaran ,  James Harper ,  Johanna Bobrow ,  Mark A. Hollis ,  Drew Chapman Brown ,  Eric Scott Clasen ,  John Calvin Schmidt

IPC分类号： C12Q1/68 ,  B01D35/00 ,  B01L99/00

CPC分类号： B01L3/502 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/042 ,  B01L2300/043 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/048 ,  B01L2300/0609 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L2400/0644 ,  B01L2400/0683 ,  C07H21/04 ,  C12N15/1013

摘要： An apparatus for preparing a nucleic acid component of a sample for amplification includes a porous support having an agent that deactivates a nucleic acid amplification inhibitor component of the sample. The apparatus further includes a housing with an opening and defining an interior. The interior of the housing is in fluid communication with the porous support, and at least a portion of a fluid directed through the opening is directed through at least a portion of the porous support. The apparatus also includes a magnetic substrate for separating nucleic acid from a sample.

摘要翻译： 用于制备用于扩增的样品的核酸组分的装置包括具有使样品的核酸扩增抑制剂组分失活的试剂的多孔载体。 该装置还包括具有开口并限定内部的壳体。 壳体的内部与多孔支撑件流体连通，并且引导通过开口的流体的至少一部分被引导通过多孔支撑件的至少一部分。 该装置还包括用于从样品中分离核酸的磁性底物。

公开(公告)号：US07217513B2

公开(公告)日：2007-05-15

申请号：US10193742

申请日：2002-07-10

申请人： Lalitha Parameswaran ,  Albert Young ,  Laura T. Bortolin ,  Mark A. Hollis ,  James Harper ,  Johanna Bobrow

发明人： Lalitha Parameswaran ,  Albert Young ,  Laura T. Bortolin ,  Mark A. Hollis ,  James Harper ,  Johanna Bobrow

IPC分类号： C12Q1/68

CPC分类号： B01L3/0282 ,  B01L3/0241 ,  B01L3/5023 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/049 ,  C12Q1/6806 ,  C12Q2565/137 ,  C12Q2549/125

摘要： A method for preparing a nucleic acid component of a sample for amplification includes contacting the sample with a porous support that deactivates a nucleic acid amplification inhibitor component of the sample and directing a fluid through the porous support, whereby the nucleic acid component of the sample is directed through at least a portion of the porous support and is separated from the support, thereby preparing the nucleic acid component for amplification. The method can be conducted in an apparatus that includes a porous support having a component that deactivates a nucleic acid amplification inhibitor component of a sample contacting the porous support and a housing having an opening and defining an interior, said interior being in fluid communication with the porous support, whereby at least a portion of a fluid directed through the opening is directed through at least a portion of the porous support and separates at least a portion of a nucleic acid component of a sample contacting the porous support from the support, thereby preparing the nucleic acid component for amplification.

摘要翻译： 用于制备用于扩增的样品的核酸组分的方法包括使样品与多孔载体接触，多孔载体使样品的核酸扩增抑制剂组分失活并引导流体通过多孔载体，由此样品的核酸组分为 引导通过多孔载体的至少一部分并与载体分离，由此制备用于扩增的核酸组分。 该方法可以在包括多孔载体的装置中进行，所述多孔载体具有使与多孔载体接触的样品的核酸扩增抑制剂组分失活的组分和具有开口并限定内部的壳体，所述内部与 多孔载体，其中通过开口引导的流体的至少一部分被引导通过多孔载体的至少一部分，并将与多孔载体接触的样品的核酸组分的至少一部分与载体分离，由此制备 用于扩增的核酸组分。

公开(公告)号：US09991113B2

公开(公告)日：2018-06-05

申请号：US15613396

申请日：2017-06-05

申请人： Jeehwan Kim ,  Dirk Robert Englund ,  Mark A. Hollis ,  Travis Wade ,  Michael Geis ,  Richard Molnar

发明人： Jeehwan Kim ,  Dirk Robert Englund ,  Mark A. Hollis ,  Travis Wade ,  Michael Geis ,  Richard Molnar

IPC分类号： H01L21/20 ,  H01L21/02 ,  H01L21/306 ,  H01L21/3213 ,  H01L21/683 ,  H01L29/04 ,  H01L29/16

CPC分类号： H01L21/02527 ,  H01L21/02376 ,  H01L21/02444 ,  H01L21/0245 ,  H01L21/02499 ,  H01L21/02598 ,  H01L21/0262 ,  H01L21/02639 ,  H01L21/02645 ,  H01L21/02647 ,  H01L21/30604 ,  H01L21/32133 ,  H01L21/6835 ,  H01L29/04 ,  H01L29/1602 ,  H01L29/1606 ,  H01L29/66015 ,  H01L2221/68363 ,  H01L2221/68381 ,  H01L2221/68386

摘要： A buffer layer is employed to fabricate diamond membranes and allow reuse of diamond substrates. In this approach, diamond membranes are fabricated on the buffer layer, which in turn is disposed on a diamond substrate that is lattice-matched to the diamond membrane. The weak bonding between the buffer layer and the diamond substrate allows ready release of the fabricated diamond membrane. The released diamond membrane is transferred to another substrate to fabricate diamond devices, while the diamond substrate is reused for another fabrication.

公开(公告)号：US5168069A

公开(公告)日：1992-12-01

申请号：US312133

申请日：1989-02-17

申请人： Frank W. Smith ,  Mark A. Hollis ,  Arthur R. Calawa ,  Vicky Diadiuk ,  Han Q. Le

发明人： Frank W. Smith ,  Mark A. Hollis ,  Arthur R. Calawa ,  Vicky Diadiuk ,  Han Q. Le

IPC分类号： H01L21/20 ,  H01L21/285 ,  H01L21/335 ,  H01L21/337 ,  H01L21/338 ,  H01L21/8252 ,  H01L29/10 ,  H01L29/51 ,  H01L29/812 ,  H01L31/09 ,  H01L31/18 ,  H01S5/02 ,  H01S5/026 ,  H01S5/223

CPC分类号： H01L29/517 ,  H01L21/02395 ,  H01L21/02463 ,  H01L21/02546 ,  H01L21/02631 ,  H01L21/02634 ,  H01L21/28587 ,  H01L21/8252 ,  H01L29/1075 ,  H01L29/66462 ,  H01L29/66863 ,  H01L29/66924 ,  H01L29/8128 ,  H01L31/09 ,  H01L31/184 ,  H01S5/0208 ,  H01S5/021 ,  H01S5/026 ,  H01S5/223 ,  Y02E10/544 ,  Y02P70/521 ,  Y10S148/003 ,  Y10S148/025 ,  Y10S148/041 ,  Y10S148/056 ,  Y10S148/154 ,  Y10S438/971

摘要： An ultra-high-speed photoconductive device is described which comprises a homoepitaxial semi-insulating III-V layer, or body, upon which ohmic/conductive contacts, or strips, separated by a small gap, are formed. The semi-insulating body, or layer, is produced by low temperature growth of III-V compounds by MBE. In a GaAs embodiment, the layer is grown under arsenic stable growth conditions, at a substrate temperature preferably in the range of 150.degree. to about 300.degree. C.

摘要翻译： 描述了一种超高速光电导器件，其包括同质外延半绝缘III-V层或体，在其上形成由小间隙分开的欧姆/导电触点或条带。 半绝缘体或层是通过MBE的III-V化合物的低温生长而产生的。 在GaAs实施方案中，该层在砷稳定生长条件下生长，在衬底温度下优选在150℃至约300℃的范围内。

公开(公告)号：US20100041031A1

公开(公告)日：2010-02-18

申请号：US12217471

申请日：2008-07-03

申请人： Eric D. Schwoebel ,  James D. Harper ,  Martha S. Petrovick ,  Frances E. Nargi ,  Todd H. Rider ,  Kristine E. Hogan ,  Richard H. Mathews ,  Joseph Lacirignola ,  Mark Hennessy ,  Trina R. Vian ,  Rose M. Joseph ,  Raymond S. Uttaro ,  Shaun Berry ,  Bernadette Johnson ,  Mark A. Hollis

发明人： Eric D. Schwoebel ,  James D. Harper ,  Martha S. Petrovick ,  Frances E. Nargi ,  Todd H. Rider ,  Kristine E. Hogan ,  Richard H. Mathews ,  Joseph Lacirignola ,  Mark Hennessy ,  Trina R. Vian ,  Rose M. Joseph ,  Raymond S. Uttaro ,  Shaun Berry ,  Bernadette Johnson ,  Mark A. Hollis

IPC分类号： C12Q1/68 ,  G01N33/566 ,  G01N21/00 ,  C12M1/34

CPC分类号： G01N33/5304 ,  C12M1/3461 ,  C12M1/3476 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/582 ,  C12Q2565/619 ,  C12Q2563/131

摘要： The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor cell comprising an Fc receptor and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more antibodies. Also provided is an optoelectronic sensor device for detecting a target particle in a plurality of samples.

摘要翻译： 本文描述的本发明提供了用于检测可溶性抗原的方法。 特别地，该方法提供了可溶性蛋白质和化学物质的检测。 此外，本发明提供了检测样品中核酸序列的方法。 还描述了包含Fc受体和发射体分子的发射体细胞，用于检测样品中的靶颗粒，其中待检测的靶颗粒被一种或多种抗体结合。 还提供了一种用于检测多个样品中的目标颗粒的光电传感器装置。

公开(公告)号：US09671670B2

公开(公告)日：2017-06-06

申请号：US14294422

申请日：2014-06-03

申请人： Mark A. Hollis ,  Reuel B. Swint ,  Dominic Siriani ,  Joseph P. Donnelly ,  Paul William Juodawlkis

发明人： Mark A. Hollis ,  Reuel B. Swint ,  Dominic Siriani ,  Joseph P. Donnelly ,  Paul William Juodawlkis

IPC分类号： G02F1/035 ,  G02F1/225 ,  G02B6/14 ,  G02F1/017 ,  G02B6/12 ,  G02F1/21

CPC分类号： G02F1/2255 ,  G02B6/14 ,  G02B2006/12097 ,  G02F1/01708 ,  G02F1/01716 ,  G02F1/2257 ,  G02F2001/212 ,  G02F2201/12

摘要： An electro-optic modulator imparts the information contained in an electrical signal traveling along a transmission line onto an optical carrier by using signal-related variations in the electrical signal's voltage to modulate the refractive index or absorption in an electro-optic material through which the optical carrier propagates. For optimal bandwidth and modulation efficiency, the microwave and optical waves should be matched in velocity. However, conventional microwave transmission lines have a microwave velocity that is somewhat higher than the optical group velocity in typical optical waveguides. Tuning a microwave transmission line's capacitance reduces the microwave velocity, but also reduces the impedance below the 50Ω impedance of most microwave components. Conversely, tuning the microwave transmission line's inductance makes it possible to match the microwave velocity to the optical group velocity over bandwidths of 100 GHz or greater while maintaining a microwave impedance of 50Ω.

公开(公告)号：US08835127B2

公开(公告)日：2014-09-16

申请号：US13267276

申请日：2011-10-06

申请人： Eric D. Schwoebel ,  James D. Harper ,  Martha S. Petrovick ,  Frances E. Nargi ,  Todd H. Rider ,  Kristine E. Hogan ,  Richard H. Mathews ,  Joseph Lacirignola ,  Mark Hennessy ,  Trina R. Vian ,  Rose M. Joseph ,  Raymond S. Uttaro ,  Shaun Berry ,  Bernadette Johnson ,  Mark A. Hollis

发明人： Eric D. Schwoebel ,  James D. Harper ,  Martha S. Petrovick ,  Frances E. Nargi ,  Todd H. Rider ,  Kristine E. Hogan ,  Richard H. Mathews ,  Joseph Lacirignola ,  Mark Hennessy ,  Trina R. Vian ,  Rose M. Joseph ,  Raymond S. Uttaro ,  Shaun Berry ,  Bernadette Johnson ,  Mark A. Hollis

IPC分类号： G01N33/53 ,  C12Q1/68 ,  G01N21/64 ,  G01N33/58

CPC分类号： G01N33/5304 ,  C12M1/3461 ,  C12M1/3476 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/582 ,  C12Q2565/619 ,  C12Q2563/131

摘要： The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor cell comprising an Fc receptor and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more antibodies. Also provided is an optoelectronic sensor device for detecting a target particle in a plurality of samples.

摘要翻译： 本文描述的本发明提供了用于检测可溶性抗原的方法。 特别地，该方法提供了可溶性蛋白质和化学物质的检测。 此外，本发明提供了检测样品中核酸序列的方法。 还描述了包含Fc受体和发射体分子的发射体细胞，用于检测样品中的靶颗粒，其中待检测的靶颗粒被一种或多种抗体结合。 还提供了一种用于检测多个样品中的目标颗粒的光电传感器装置。

公开(公告)号：US20080166705A1

公开(公告)日：2008-07-10

申请号：US11001583

申请日：2004-12-01

申请人： Eric D. Schwoebel ,  James D. Harper ,  Martha S. Petrovick ,  Frances E. Nargi ,  Todd H. Rider ,  Kristine E. Hogan ,  Richard H. Mathews ,  Joseph Lacirignola ,  Mark Hennessy ,  Trina R. Vian ,  Rose M. Joseph ,  Raymond S. Uttaro ,  Shaun Berry ,  Bernadette Johnson ,  Mark A. Hollis

发明人： Eric D. Schwoebel ,  James D. Harper ,  Martha S. Petrovick ,  Frances E. Nargi ,  Todd H. Rider ,  Kristine E. Hogan ,  Richard H. Mathews ,  Joseph Lacirignola ,  Mark Hennessy ,  Trina R. Vian ,  Rose M. Joseph ,  Raymond S. Uttaro ,  Shaun Berry ,  Bernadette Johnson ,  Mark A. Hollis

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/34

CPC分类号： G01N33/5304 ,  C12M1/3461 ,  C12M1/3476 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/582 ,  C12Q2565/619 ,  C12Q2563/131

摘要： The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor cell comprising an Fc receptor and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more antibodies. Also provided is an optoelectronic sensor device for detecting a target particle in a plurality of samples.

摘要翻译： 本文描述的本发明提供了用于检测可溶性抗原的方法。 特别地，该方法提供了可溶性蛋白质和化学物质的检测。 此外，本发明提供了检测样品中核酸序列的方法。 还描述了包含Fc受体和发射体分子的发射体细胞，用于检测样品中的靶颗粒，其中待检测的靶颗粒被一种或多种抗体结合。 还提供了一种用于检测多个样品中的目标颗粒的光电传感器装置。

公开(公告)号：US5332918A

公开(公告)日：1994-07-26

申请号：US938385

申请日：1992-08-31

申请人： Frank W. Smith ,  Mark A. Hollis ,  Arthur R. Calawa ,  Vicky Diadiuk ,  Han Q. Le

发明人： Frank W. Smith ,  Mark A. Hollis ,  Arthur R. Calawa ,  Vicky Diadiuk ,  Han Q. Le

IPC分类号： H01L21/20 ,  H01L21/285 ,  H01L21/335 ,  H01L21/337 ,  H01L21/338 ,  H01L21/8252 ,  H01L29/10 ,  H01L29/51 ,  H01L29/812 ,  H01L31/09 ,  H01L31/18 ,  H01S5/02 ,  H01S5/026 ,  H01S5/223 ,  H01L27/14 ,  H01L23/485 ,  H01L31/00

CPC分类号： H01L29/517 ,  H01L21/28587 ,  H01L21/8252 ,  H01L29/1075 ,  H01L29/66462 ,  H01L29/66863 ,  H01L29/66924 ,  H01L29/8128 ,  H01L31/09 ,  H01L31/184 ,  H01L21/02395 ,  H01L21/02463 ,  H01L21/02546 ,  H01L21/02631 ,  H01S5/0208 ,  H01S5/021 ,  H01S5/026 ,  H01S5/223 ,  Y02E10/544

摘要： An ultra-high-speed photoconductive device is described which comprises a homoepitaxial semi-insulating III-V layer, or body, upon which ohmic/conductive contacts, or strips, separated by a small gap, are formed. The semi-insulating body, or layer, is produced by low temperature growth of III-V compounds by MBE. In a GaAs embodiment, the layer is grown under arsenic stable growth conditions, at a substrate temperature preferably in the range of 150.degree. to about 300.degree. C.

摘要翻译： 描述了一种超高速光电导器件，其包括同质外延半绝缘III-V层或体，在其上形成由小间隙分开的欧姆/导电触点或条带。 半绝缘体或层是通过MBE的III-V化合物的低温生长而产生的。 在GaAs实施方案中，该层在砷稳定生长条件下生长，在衬底温度下优选在150℃至约300℃的范围内。