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公开(公告)号：US07025967B2
公开(公告)日：2006-04-11
申请号：US10046938
申请日：2002-01-14
IPC分类号： A61K39/235 , A61K39/295 , C12N7/01 , C12N15/861
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention relates novel live bovine adenovirus (BAV) expression vector systems in which part or all of one or both of the early region 1 (E1) and early region 3 (E3) genes are deleted and replaced by a foreign gene or fragment thereof and novel recombinant mammalian cell lines stably transformed with BAV E1 sequences, and therefore, express E1 gene products capable of allowing replication therein of a bovine adenovirus having an E1 deletion replaced by a heterologous nucleotide sequence encoding a foreign gene or fragment thereof and their use in production of (antigenic) polypeptides or fragments thereof for the purpose of live recombinant virus or subunit vaccine or for other therapies.
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公开(公告)号：US6086890A
公开(公告)日：2000-07-11
申请号：US815927
申请日：1997-03-13
IPC分类号： A61K48/00 , C07K14/075 , C12N7/01 , C12N15/861 , A61K39/235 , C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention relates novel live bovine adenovirus (BAV) expression vector systems in which part or all of one or both of the early region 1 (E1) and early region 3 (E3) genes are deleted and replaced by a foreign gene or fragment thereof and novel recombinant mammalian cell lines stably transformed with BAV E1 sequences, and therefore, express E1 gene products capable of allowing replication therein of a bovine adenovirus having an E1 deletion replaced by a heterologous nucleotide sequence encoding a foreign gene or fragment thereof and their use in production of (antigenic) polypeptides or fragments thereof for the purpose of live recombinant virus or subunit vaccine or for other therapies.
摘要翻译： 本发明涉及新鲜活牛腺病毒（BAV）表达载体系统，其中早期区域1（E1）和早期区域3（E3）基因中的一个或两个的部分或全部缺失并被外源基因或其片段替代 以及用BAV E1序列稳定转化的新型重组哺乳动物细胞系，因此表达能够允许在其中复制具有E1缺失的牛腺病毒的E1基因产物，其被编码外源基因或其片段的异源核苷酸序列替代，以及它们在 用于活重组病毒或亚单位疫苗或用于其他疗法的（抗原性）多肽或其片段的产生。
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公开(公告)号：US6001591A
公开(公告)日：1999-12-14
申请号：US845623
申请日：1997-04-25
IPC分类号： A61K48/00 , C07K14/075 , C12N7/01 , C12N15/861 , C12N15/00 , C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention provides recombinant bovine adenovirus (BAV) vectors and expression systems for introduction and expression of non-BAV sequences in mammalian cells. Methods for the preparation and use of these vectors and expression systems are also provided.
摘要翻译： 本发明提供重组牛腺病毒（BAV）载体和用于在哺乳动物细胞中引入和表达非BAV序列的表达系统。 还提供了用于制备和使用这些载体和表达系统的方法。
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公开(公告)号：US06379944B1
公开(公告)日：2002-04-30
申请号：US09435242
申请日：1999-11-05
IPC分类号： C12N700
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention provides novel recombinant mammalian cell lines expressing bovine adenovirus functions. In one embodiment, mammalian cell lines stably transformed with bovine adenovirus E1 sequences express bovine adenovirus E1 gene products and are useful for the replication of bovine adenovirus vectors having deletions and/or insertions in the E1 region. Such vector/cell line combinations are useful for synthesis of recombinant polypeptides, preparation of subunit vaccines, and in gene therapy.
摘要翻译： 本发明提供了表达牛腺病毒功能的新型重组哺乳动物细胞系。 在一个实施方案中，用牛腺病毒E1序列稳定转化的哺乳动物细胞系表达牛腺病毒E1基因产物，并且可用于在E1区域中具有缺失和/或插入的牛腺病毒载体的复制。 这样的载体/细胞系组合可用于合成重组多肽，亚单位疫苗的制备和基因治疗。
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公开(公告)号：US6140087A
公开(公告)日：2000-10-31
申请号：US250885
申请日：1994-05-31
申请人： Frank L. Graham , Andrew Bett , Ludvik Prevec , Wael M. Haddara
发明人： Frank L. Graham , Andrew Bett , Ludvik Prevec , Wael M. Haddara
CPC分类号： C12N15/86 , A01K2217/05 , C12N2710/10343 , C12N2800/30
摘要： The invention comprises a series of adenovirus-based vectors having deletions in the E1 and/or E3 regions, and also insertions of pBR322 sequences, which can be used to deliver nucleic acid inserts into host cells, tissues or organisms that then can express the insert. The invention includes the use of the vectors in introducing genes into cells, in making vaccines and in gene therapy.
摘要翻译： 本发明包括在E1和/或E3区域中具有缺失的一系列基于腺病毒的载体，以及pBR322序列的插入，其可用于将核酸插入物递送到宿主细胞，组织或生物体中，然后可以表达插入物 。 本发明包括使用载体将基因导入细胞，制备疫苗和进行基因治疗。
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公开(公告)号：US5820868A
公开(公告)日：1998-10-13
申请号：US164292
申请日：1993-12-09
IPC分类号： A61K48/00 , C07K14/075 , C12N7/01 , C12N15/861 , A61K39/235 , C12N15/00
CPC分类号： C12N15/86 , C07K14/005 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention relates novel live bovine adenovirus (BAV) expression vector systems in which part or all of one or both of the early region 1 (E1) and early region 3 (E3) genes are deleted and replaced by a foreign gene or fragment thereof and novel recombinant mammalian cell lines stably transformed with BAV E1 sequences, and therefore, express E1 gene products capable of allowing replication therein of a bovine adenovirus having an E1 deletion replaced by a heterologous nucleotide sequence encoding a foreign gene or fragment thereof and their use in production of (antigenic) polypeptides or fragments thereof for the purpose of live recombinant virus or subunit vaccine or for other therapies.
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