公开(公告)号：US20130295126A1

公开(公告)日：2013-11-07

申请号：US13943990

申请日：2013-07-17

Applicant: Susantha Gomis ,  Suresh Kumar Tikoo ,  Davor Ojkic

Inventor： Susantha Gomis ,  Suresh Kumar Tikoo ,  Davor Ojkic

IPC: A61K39/235

CPC classification number: A61K39/235 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/542 ,  A61K2039/552 ,  A61K2039/55561 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2710/10034 ,  C12N2710/10234 ,  C12N2710/10251

Abstract: A composition comprising an isolated fowl adenovirus (FAdV), wherein the FAdV is a strain selected from FAdV-2, FAdV-7, FAdv-8a, FAdV-8b, FAdV-8a/8b or FAdV-11 serotype strains; and a suitable carrier and methods for inducing protective immunity in a subject and/or its progeny.

Abstract translation: 一种包含分离的禽腺病毒（FAdV）的组合物，其中所述FAdV是选自FAdV-2，FAdV-7，FAdv-8a，FAdV-8b，FAdV-8a / 8b或FAdV-11血清型毒株的菌株; 以及在受试者和/或其后代中诱导保护性免疫的合适的载体和方法。

公开(公告)号：US08524249B2

公开(公告)日：2013-09-03

申请号：US12969444

申请日：2010-12-15

Applicant: Susantha Gomis ,  Suresh Kumar Tikoo ,  Davor Ojkic

Inventor： Susantha Gomis ,  Suresh Kumar Tikoo ,  Davor Ojkic

IPC: A61K39/295 ,  A61K39/235

CPC classification number: A61K39/235 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  A61K2039/542 ,  A61K2039/552 ,  A61K2039/55561 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2710/10034 ,  C12N2710/10234 ,  C12N2710/10251

Abstract: A composition comprising an isolated fowl adenovirus (FAdV), wherein the FAdV is a strain selected from FAdV-2, FAdV-7, FAdv-8a, FAdV-8b, FAdV-8a/8b or FAdV-11 serotype strains; and a suitable carrier and methods for inducing protective immunity in a subject and/or its progeny.

Abstract translation: 一种包含分离的禽腺病毒（FAdV）的组合物，其中所述FAdV是选自FAdV-2，FAdV-7，FAdv-8a，FAdV-8b，FAdV-8a / 8b或FAdV-11血清型毒株的菌株; 以及在受试者和/或其后代中诱导保护性免疫的合适的载体和方法。

公开(公告)号：US06492343B1

公开(公告)日：2002-12-10

申请号：US09292034

申请日：1999-04-14

Applicant: Police Seshidhar Reddy ,  Suresh Kumar Tikoo ,  Lorne A. Babiuk

Inventor： Police Seshidhar Reddy ,  Suresh Kumar Tikoo ,  Lorne A. Babiuk

IPC: A01N4304

CPC classification number: C12N15/86 ,  A61K48/00 ,  A61K2039/53 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10362

Abstract: The complete nucleotide sequence of the genome of porcine adenovirus type 3 (PAV-3) is provided. Methods for construction of infectious PAV genomes by homologous recombination in procaryotic cells are provided. Recombinant PAV viruses are obtained by transfection of mammalian cells with recombinant PAV genomes. The PAV-3 genome can be used as a vector for the expression of heterologous nucleotide sequences, for example, for the preparation and administration of subunit vaccines to swine or other mammals. In addition, PAV-3 vectors can be used for gene therapy and expression of heterologous polypeptides. PAV-3 genome sequences can also be used for diagnostic purposes, to detect the presence of PAV-3 DNA in a subject or biological sample.

Abstract translation: 提供猪腺病毒3型（PAV-3）基因组的完整核苷酸序列。 提供了在原核细胞中通过同源重组构建感染性PAV基因组的方法。 通过用重组PAV基因组转染哺乳动物细胞获得重组PAV病毒。 PAV-3基因组可用作异源核苷酸序列表达的载体，例如用于制备和施用猪或其他哺乳动物的亚单位疫苗。 此外，PAV-3载体可用于基因治疗和异源多肽的表达。 PAV-3基因组序列也可用于诊断目的，以检测受试者或生物样品中PAV-3 DNA的存在。

公开(公告)号：US06458586B1

公开(公告)日：2002-10-01

申请号：US09423156

申请日：1999-11-01

Applicant: Suresh Kumar Tikoo ,  Lorne A. Babiuk ,  Police Seshidhar Reddy

Inventor： Suresh Kumar Tikoo ,  Lorne A. Babiuk ,  Police Seshidhar Reddy

IPC: C12N506

CPC classification number: C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10352 ,  Y10S424/813

Abstract: The invention provides cell lines capable of supporting the replication of a defective recombinant virus vector. In one aspect, bovine cell lines expressing adenovirus E1 functions are provided. The cell lines are useful for the propagation of adenovirus vectors with mutations and/or deletions in E1 and other essential regions of the adenovirus genome.

Abstract translation: 本发明提供能够支持有缺陷的重组病毒载体的复制的细胞系。 一方面，提供了表达腺病毒E1功能的牛细胞系。 细胞系可用于在E1和腺病毒基因组的其他必需区域中扩增具有突变和/或缺失的腺病毒载体。

公开(公告)号：US06379944B1

公开(公告)日：2002-04-30

申请号：US09435242

申请日：1999-11-05

Applicant: Suresh K. Mittal ,  Frank L. Graham ,  Ludvik Prevec ,  Lorne A. Babiuk ,  Suresh Kumar Tikoo

Inventor： Suresh K. Mittal ,  Frank L. Graham ,  Ludvik Prevec ,  Lorne A. Babiuk ,  Suresh Kumar Tikoo

IPC: C12N700

CPC classification number: C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352

Abstract: The present invention provides novel recombinant mammalian cell lines expressing bovine adenovirus functions. In one embodiment, mammalian cell lines stably transformed with bovine adenovirus E1 sequences express bovine adenovirus E1 gene products and are useful for the replication of bovine adenovirus vectors having deletions and/or insertions in the E1 region. Such vector/cell line combinations are useful for synthesis of recombinant polypeptides, preparation of subunit vaccines, and in gene therapy.

Abstract translation: 本发明提供了表达牛腺病毒功能的新型重组哺乳动物细胞系。 在一个实施方案中，用牛腺病毒E1序列稳定转化的哺乳动物细胞系表达牛腺病毒E1基因产物，并且可用于在E1区域中具有缺失和/或插入的牛腺病毒载体的复制。 这样的载体/细胞系组合可用于合成重组多肽，亚单位疫苗的制备和基因治疗。

公开(公告)号：US06319716B1

公开(公告)日：2001-11-20

申请号：US09103330

申请日：1998-06-23

Applicant: Suresh Kumar Tikoo ,  Lorne A. Babiuk ,  Police Seshidhar Reddy ,  Alexandre Zakhartchouk ,  Mohit Baxi

Inventor： Suresh Kumar Tikoo ,  Lorne A. Babiuk ,  Police Seshidhar Reddy ,  Alexandre Zakhartchouk ,  Mohit Baxi

IPC: C12N15181

CPC classification number: C12N15/86 ,  A61K39/00 ,  A61K2039/51 ,  C12N2710/10321 ,  C12N2710/10322 ,  C12N2710/10343

Abstract: The present invention provides the complete nucleotide sequence of a bovine adenovirus. The invention further provides bovine adenovirus vectors and expression systems which can be used, among other things, for insertion of foreign sequences, for provision of DNA control sequences including transcriptional and translational regulatory sequences, for diagnostic purposes to detect the presence of viral nucleic acids or proteins encoded by these regions in a subject or biological sample, for provision of immunogenic polypeptides or fragments thereof, for vaccines and for gene therapy. Cell lines comprising the vectors of the invention, and methods for making bovine adenovirus vectors are also provided.

Abstract translation: 本发明提供了牛腺病毒的完整核苷酸序列。 本发明进一步提供牛腺病毒载体和表达系统，其可以用于插入外来序列，用于提供包括转录和翻译调节序列的DNA控制序列，用于诊断目的以检测病毒核酸的存在或 由受试者或生物样品中的这些区域编码的蛋白质，用于提供免疫原性多肽或其片段，用于疫苗和用于基因治疗。 还提供了包含本发明载体的细胞系和制备牛腺病毒载体的方法。