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公开(公告)号：US07025967B2
公开(公告)日：2006-04-11
申请号：US10046938
申请日：2002-01-14
IPC分类号： A61K39/235 , A61K39/295 , C12N7/01 , C12N15/861
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention relates novel live bovine adenovirus (BAV) expression vector systems in which part or all of one or both of the early region 1 (E1) and early region 3 (E3) genes are deleted and replaced by a foreign gene or fragment thereof and novel recombinant mammalian cell lines stably transformed with BAV E1 sequences, and therefore, express E1 gene products capable of allowing replication therein of a bovine adenovirus having an E1 deletion replaced by a heterologous nucleotide sequence encoding a foreign gene or fragment thereof and their use in production of (antigenic) polypeptides or fragments thereof for the purpose of live recombinant virus or subunit vaccine or for other therapies.
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公开(公告)号：US6086890A
公开(公告)日：2000-07-11
申请号：US815927
申请日：1997-03-13
IPC分类号： A61K48/00 , C07K14/075 , C12N7/01 , C12N15/861 , A61K39/235 , C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention relates novel live bovine adenovirus (BAV) expression vector systems in which part or all of one or both of the early region 1 (E1) and early region 3 (E3) genes are deleted and replaced by a foreign gene or fragment thereof and novel recombinant mammalian cell lines stably transformed with BAV E1 sequences, and therefore, express E1 gene products capable of allowing replication therein of a bovine adenovirus having an E1 deletion replaced by a heterologous nucleotide sequence encoding a foreign gene or fragment thereof and their use in production of (antigenic) polypeptides or fragments thereof for the purpose of live recombinant virus or subunit vaccine or for other therapies.
摘要翻译： 本发明涉及新鲜活牛腺病毒（BAV）表达载体系统，其中早期区域1（E1）和早期区域3（E3）基因中的一个或两个的部分或全部缺失并被外源基因或其片段替代 以及用BAV E1序列稳定转化的新型重组哺乳动物细胞系，因此表达能够允许在其中复制具有E1缺失的牛腺病毒的E1基因产物，其被编码外源基因或其片段的异源核苷酸序列替代，以及它们在 用于活重组病毒或亚单位疫苗或用于其他疗法的（抗原性）多肽或其片段的产生。
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公开(公告)号：US6001591A
公开(公告)日：1999-12-14
申请号：US845623
申请日：1997-04-25
IPC分类号： A61K48/00 , C07K14/075 , C12N7/01 , C12N15/861 , C12N15/00 , C12N15/86
CPC分类号： C12N15/86 , C07K14/005 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention provides recombinant bovine adenovirus (BAV) vectors and expression systems for introduction and expression of non-BAV sequences in mammalian cells. Methods for the preparation and use of these vectors and expression systems are also provided.
摘要翻译： 本发明提供重组牛腺病毒（BAV）载体和用于在哺乳动物细胞中引入和表达非BAV序列的表达系统。 还提供了用于制备和使用这些载体和表达系统的方法。
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公开(公告)号：US06379944B1
公开(公告)日：2002-04-30
申请号：US09435242
申请日：1999-11-05
IPC分类号： C12N700
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention provides novel recombinant mammalian cell lines expressing bovine adenovirus functions. In one embodiment, mammalian cell lines stably transformed with bovine adenovirus E1 sequences express bovine adenovirus E1 gene products and are useful for the replication of bovine adenovirus vectors having deletions and/or insertions in the E1 region. Such vector/cell line combinations are useful for synthesis of recombinant polypeptides, preparation of subunit vaccines, and in gene therapy.
摘要翻译： 本发明提供了表达牛腺病毒功能的新型重组哺乳动物细胞系。 在一个实施方案中，用牛腺病毒E1序列稳定转化的哺乳动物细胞系表达牛腺病毒E1基因产物，并且可用于在E1区域中具有缺失和/或插入的牛腺病毒载体的复制。 这样的载体/细胞系组合可用于合成重组多肽，亚单位疫苗的制备和基因治疗。
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公开(公告)号：US5820868A
公开(公告)日：1998-10-13
申请号：US164292
申请日：1993-12-09
IPC分类号： A61K48/00 , C07K14/075 , C12N7/01 , C12N15/861 , A61K39/235 , C12N15/00
CPC分类号： C12N15/86 , C07K14/005 , A61K48/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10352
摘要： The present invention relates novel live bovine adenovirus (BAV) expression vector systems in which part or all of one or both of the early region 1 (E1) and early region 3 (E3) genes are deleted and replaced by a foreign gene or fragment thereof and novel recombinant mammalian cell lines stably transformed with BAV E1 sequences, and therefore, express E1 gene products capable of allowing replication therein of a bovine adenovirus having an E1 deletion replaced by a heterologous nucleotide sequence encoding a foreign gene or fragment thereof and their use in production of (antigenic) polypeptides or fragments thereof for the purpose of live recombinant virus or subunit vaccine or for other therapies.
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6.发明授权公开(公告)号：US07504206B2
公开(公告)日：2009-03-17
申请号：US11217562
申请日：2005-09-01
申请人： Deborah Haines , Gordon Allan , John Ellis , Brian Meehan , Edward Clark , Lori Hassard , John Harding , Catherine Elisabeth Charreyre , Gilles Emile Chappuis , Francis McNeilly , Li Wang , Lorne A. Babiuk , Andrew A. Potter , Philip Willson
发明人： Deborah Haines , Gordon Allan , John Ellis , Brian Meehan , Edward Clark , Lori Hassard , John Harding , Catherine Elisabeth Charreyre , Gilles Emile Chappuis , Francis McNeilly , Li Wang , Lorne A. Babiuk , Andrew A. Potter , Philip Willson
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , A61K39/12 , A61K48/00 , A61K2039/51 , A61K2039/53 , A61K2039/552 , A61K2039/70 , C12N7/00 , C12N15/86 , C12N2750/10022 , C12N2750/10034 , C12N2750/10043 , C12Q1/701
摘要： The cloning of a novel PCVII viral genome is described as is expression of proteins derived from the PCVII genome. These proteins can be used in vaccine compositions for the prevention and treatment of PCVII infections, as well as in diagnostic methods for determining the presence of PCVII infections in a vertebrate subject. Polynucleotides derived from the viral genome can be used as diagnostic primers and probes.
摘要翻译： 新型PCVII病毒基因组的克隆描述为源自PCVII基因组的蛋白质的表达。 这些蛋白质可用于预防和治疗PCVII感染的疫苗组合物中，以及用于确定脊椎动物受试者中PCVII感染存在的诊断方法。 衍生自病毒基因组的多核苷酸可用作诊断引物和探针。
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7.发明授权公开(公告)号：US07192594B2
公开(公告)日：2007-03-20
申请号：US10653849
申请日：2003-09-02
申请人： Deborah Haines , Gordon Allan , John Ellis , Brian Meehan , Edward Clark , Lori Hassard , John Harding , Catherine Elisabeth Charreyre , Gilles Emile Chappuis , Francis McNeilly , Li Wang , Lorne A. Babiuk , Andrew A. Potter , Philip Willson
发明人： Deborah Haines , Gordon Allan , John Ellis , Brian Meehan , Edward Clark , Lori Hassard , John Harding , Catherine Elisabeth Charreyre , Gilles Emile Chappuis , Francis McNeilly , Li Wang , Lorne A. Babiuk , Andrew A. Potter , Philip Willson
IPC分类号： A61K39/12
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , A61K39/12 , A61K48/00 , A61K2039/51 , A61K2039/53 , A61K2039/552 , A61K2039/70 , C12N7/00 , C12N15/86 , C12N2750/10022 , C12N2750/10034 , C12N2750/10043 , C12Q1/701
摘要： The cloning of a novel PCVII viral genome is described as is expression of proteins derived from the PCVII genome. These proteins can be used in vaccine compositions for the prevention and treatment of PCVII infections, as well as in diagnostic methods for determining the presence of PCVII infections in a vertebrate subject. Polynucleotides derived from the viral genome can be used as diagnostic primers and probes.
摘要翻译： 新型PCVII病毒基因组的克隆描述为源自PCVII基因组的蛋白质的表达。 这些蛋白质可用于预防和治疗PCVII感染的疫苗组合物中，以及用于确定脊椎动物受试者中PCVII感染存在的诊断方法。 衍生自病毒基因组的多核苷酸可用作诊断引物和探针。
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公开(公告)号：US06492343B1
公开(公告)日：2002-12-10
申请号：US09292034
申请日：1999-04-14
IPC分类号： A01N4304
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/53 , C07K14/005 , C12N7/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10362
摘要： The complete nucleotide sequence of the genome of porcine adenovirus type 3 (PAV-3) is provided. Methods for construction of infectious PAV genomes by homologous recombination in procaryotic cells are provided. Recombinant PAV viruses are obtained by transfection of mammalian cells with recombinant PAV genomes. The PAV-3 genome can be used as a vector for the expression of heterologous nucleotide sequences, for example, for the preparation and administration of subunit vaccines to swine or other mammals. In addition, PAV-3 vectors can be used for gene therapy and expression of heterologous polypeptides. PAV-3 genome sequences can also be used for diagnostic purposes, to detect the presence of PAV-3 DNA in a subject or biological sample.
摘要翻译： 提供猪腺病毒3型（PAV-3）基因组的完整核苷酸序列。 提供了在原核细胞中通过同源重组构建感染性PAV基因组的方法。 通过用重组PAV基因组转染哺乳动物细胞获得重组PAV病毒。 PAV-3基因组可用作异源核苷酸序列表达的载体，例如用于制备和施用猪或其他哺乳动物的亚单位疫苗。 此外，PAV-3载体可用于基因治疗和异源多肽的表达。 PAV-3基因组序列也可用于诊断目的，以检测受试者或生物样品中PAV-3 DNA的存在。
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公开(公告)号：US07803926B2
公开(公告)日：2010-09-28
申请号：US11522237
申请日：2006-09-15
申请人： Deborah Haines , Gordon Allan , John Ellis , Brian Meehan , Edward Clark , Lori Hassard , John Harding , Catherine Elisabeth Charreyre , Gilles Emile Chappuis , Francis McNeilly , Li Wang , Lorne A. Babiuk , Andrew A. Potter , Philip Willson
发明人： Deborah Haines , Gordon Allan , John Ellis , Brian Meehan , Edward Clark , Lori Hassard , John Harding , Catherine Elisabeth Charreyre , Gilles Emile Chappuis , Francis McNeilly , Li Wang , Lorne A. Babiuk , Andrew A. Potter , Philip Willson
CPC分类号： C07K14/005 , A61K38/00 , A61K39/00 , A61K39/12 , A61K48/00 , A61K2039/51 , A61K2039/53 , A61K2039/552 , A61K2039/70 , C12N7/00 , C12N15/86 , C12N2750/10022 , C12N2750/10034 , C12N2750/10043 , C12Q1/701
摘要： The cloning of a novel PCVII viral genome is described as is expression of proteins derived from the PCVII genome. These proteins can be used in vaccine compositions for the prevention and treatment of PCVII infections, as well as in diagnostic methods for determining the presence of PCVII infections in a vertebrate subject. Polynucleotides derived from the viral genome can be used as diagnostic primers and probes.
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公开(公告)号：US06849446B2
公开(公告)日：2005-02-01
申请号：US09871212
申请日：2001-05-31
申请人： Suresh K. Tikoo , Lorne A. Babiuk , Linong Zhang , Qiaohua Wu
发明人： Suresh K. Tikoo , Lorne A. Babiuk , Linong Zhang , Qiaohua Wu
IPC分类号： C12N15/09 , A61K35/76 , A61K39/00 , A61K48/00 , A61P31/00 , A61P37/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N7/00 , C12N7/04 , C12N15/861 , C12N15/00
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/5256 , C12N7/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2710/10362 , C12N2810/6018
摘要： The present invention provides modified bovine adenoviruses comprising a modification in a capsid protein wherein said protein is associated with adenovirus tropism and wherein said modification is associated with altered tropism. The present invention provides adenovirus vectors and host cells comprising such vectors. The present invention also provides methods of making and using such adenoviruses.
摘要翻译： 本发明提供了修饰的牛腺病毒，其包含衣壳蛋白中的修饰，其中所述蛋白质与腺病毒向性相关，并且其中所述修饰与改变的嗜性相关。 本发明提供了腺病毒载体和包含这种载体的宿主细胞。 本发明还提供了制备和使用这种腺病毒的方法。
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