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1.发明申请公开(公告)号：US20100017899A1
公开(公告)日：2010-01-21
申请号：US12446801
申请日：2007-10-24
IPC分类号： A01K67/027 , C12N5/02 , C12N5/00
CPC分类号： C12N5/0606 , C12N5/0604 , C12N2500/25 , C12N2500/32 , C12N2500/40 , C12N2500/44 , C12N2500/84 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/235 , C12N2502/13
摘要： The present invention provides a method of isolating a pluripotent cell from a pre-implantation embryo without isolation of the pluripotent cells from other cells, the method including propagating a whole pre-implantation embryo including one or more pluripotent cells, embedded in a feeder cell layer and cultivated in a medium substantially free of serum, and isolating a pluripotent cell from the one or more pluripotent cells. The present invention also provides pluripotent cells generated by the method and uses thereof.
摘要翻译： 本发明提供了从植入前的胚胎中分离多能细胞而不从其它细胞分离多能细胞的方法，所述方法包括传播包含一个或多个多能细胞的整个植入前胚胎，其嵌入饲养细胞层 并在基本上不含血清的培养基中培养，并从一个或多个多能细胞中分离出多能细胞。 本发明还提供通过其方法和用途产生的多能细胞。
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公开(公告)号：US07446240B2
公开(公告)日：2008-11-04
申请号：US11532026
申请日：2006-09-14
IPC分类号： A01K67/027 , C12N15/00
CPC分类号： C12N15/8778
摘要： Methods for the production of nuclear transfer embryos, nuclear transfer embryos and animals derived therefrom are described. The method generally comprises at least the steps of: providing at least one enucleated recipient cell; providing at least one donor cell or nucleus; providing a fusion media which is substantially free of calcium; placing said at least one enculeated recipient cell and at least one donor cell or nucleus in contact with one another to form couplets; and, fusing via electrofusion in said fusion media said at least one recipient cell with at least one donor cell or nucleus to form a nuclear transfer embryo.
摘要翻译： 描述了从其衍生的核转移胚胎，核转移胚胎和动物的生产方法。 该方法通常至少包括以下步骤：提供至少一个去核的受体细胞; 提供至少一个供体细胞或细胞核; 提供基本上不含钙的融合介质; 将所述至少一个包裹的受体细胞和至少一个供体细胞或细胞核相互接触以形成对联; 并且通过所述融合介质中的电融合将所述至少一个受体细胞与至少一个供体细胞或细胞核融合以形成核转移胚。
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3.发明授权Isolating a mammalian embryonic stem cell from a homogenous pluripotent outgrowth of a mammalian pre-implantation embryo 有权将哺乳动物胚胎干细胞从哺乳动物植入前胚胎的均质多能生长中分离出来公开(公告)号：US09499785B2
公开(公告)日：2016-11-22
申请号：US12446801
申请日：2007-10-24
IPC分类号： C12N15/00 , C12N5/073 , C12N5/0735
CPC分类号： C12N5/0606 , C12N5/0604 , C12N2500/25 , C12N2500/32 , C12N2500/40 , C12N2500/44 , C12N2500/84 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/235 , C12N2502/13
摘要： The present invention provides a method of isolating a pluripotent cell from a pre-implantation embryo without isolation of the pluripotent cells from other cells, the method including propagating a whole pre-implantation embryo including one or more pluripotent cells, embedded in a feeder cell layer and cultivated in a medium substantially free of serum, and isolating a pluripotent cell from the one or more pluripotent cells. The present invention also provides pluripotent cells generated by the method and uses thereof.
摘要翻译： 本发明提供了从植入前的胚胎中分离多能细胞而不从其它细胞分离多能细胞的方法，所述方法包括传播包含一个或多个多能细胞的整个植入前胚胎，其嵌入饲养细胞层 并在基本上不含血清的培养基中培养，并从一个或多个多能细胞中分离出多能细胞。 本发明还提供通过其方法和用途产生的多能细胞。
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