公开(公告)号：US07413897B2

公开(公告)日：2008-08-19

申请号：US10387547

申请日：2003-03-14

申请人： Lola M. Reid ,  Nicholas Moss ,  Mark Furth ,  John W. Ludlow ,  Andrew T. Bruce

发明人： Lola M. Reid ,  Nicholas Moss ,  Mark Furth ,  John W. Ludlow ,  Andrew T. Bruce

IPC分类号： C12N5/00 ,  A01N63/00

CPC分类号： C12N5/0672 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2502/13 ,  G01N33/5005 ,  G01N33/68 ,  G01N2333/705 ,  G01N2333/70525 ,  G01N2333/70596

摘要： Hepatic progenitors comprise two populations of human hepatic stem cells, primitive and proximal hepatic stem cells, and two populations of committed progenitors, one for biliary cells and one for hepatocytes. Human primitive hepatic stem cells are a very small fraction of the liver cell population and give rise to proximal hepatic stem cells constituting a much larger fraction of the liver. Human proximal hepatic stem cells give rise to biliary and hepatocyte committed progenitors. Primitive and proximal stem cells are the primary stem cells for the human liver. Human primitive hepatic stem cells may be isolated by immunoselection from human livers or culturing human liver cells under conditions which select for a human primitive hepatic stem cell. Proximal hepatic stem cells may be isolated by immunoselection, or by culturing human liver cells under conditions which include a developmental factor. Proximal hepatic stem cells may also be isolated by culturing colonies comprising a primitive hepatic stem cell under conditions which include a developmental factor. Resulting compositions may be used for treating liver disorders and for producing bioartificial organs.

摘要翻译： 肝祖细胞包含两个人类肝干细胞，原始和近端肝干细胞，以及两个定型祖细胞群，一个为胆汁细胞，一个为肝细胞。 人原始肝干细胞是肝细胞群体的非常小的一部分，并且引起近端肝干细胞构成肝脏的大部分。 人近端肝干细胞产生胆汁和肝细胞定向的祖细胞。 原始和近端干细胞是人类肝脏的主要干细胞。 人原始肝干细胞可以通过人肝脏的免疫选择或在选择人原始肝干细胞的条件下培养人肝细胞来分离。 可以通过免疫选择或通过在包括发育因子的条件下培养人肝细胞来分离近端肝干细胞。 也可以通过在包括发育因子的条件下培养包含原始肝干细胞的菌落来分离近端肝干细胞。 所得组合物可用于治疗肝脏疾病和用于生产生物人造器官。

公开(公告)号：US20070099297A1

公开(公告)日：2007-05-03

申请号：US11609729

申请日：2006-12-12

申请人： Lola Reid ,  Nick Moss ,  Mark Furth ,  John Ludlow ,  Andrew Bruce

发明人： Lola Reid ,  Nick Moss ,  Mark Furth ,  John Ludlow ,  Andrew Bruce

IPC分类号： C12N5/08

CPC分类号： C12N5/0672 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2502/13 ,  G01N33/5005 ,  G01N33/68 ,  G01N2333/705 ,  G01N2333/70525 ,  G01N2333/70596

摘要： Hepatic progenitors comprise two populations of human hepatic stem cells, primitive and proximal hepatic stem cells, and two populations of committed progenitors, one for biliary cells and one for hepatocytes. Human primitive hepatic stem cells are a very small fraction of the liver cell population and give rise to proximal hepatic stem cells constituting a much larger fraction of the liver. Human proximal hepatic stem cells give rise to biliary and hepatocyte committed progenitors. Primitive and proximal stem cells are the primary stem cells for the human liver. Human primitive hepatic stem cells may be isolated by immunoselection from human livers or culturing human liver cells under conditions which select for a human primitive hepatic stem cell. Proximal hepatic stem cells may be isolated by immunoselection, or by culturing human liver cells under conditions which include a developmental factor. Proximal hepatic stem cells may also be isolated by culturing colonies comprising a primitive hepatic stem cell under conditions which include a developmental factor. Resulting compositions may be used for treating liver disorders and for producing bioartificial organs.

摘要翻译： 肝祖细胞包含两个人类肝干细胞，原始和近端肝干细胞，以及两个定型祖细胞群，一个为胆汁细胞，一个为肝细胞。 人原始肝干细胞是肝细胞群体的非常小的一部分，并且引起近端肝干细胞构成肝脏的大部分。 人近端肝干细胞产生胆汁和肝细胞定向的祖细胞。 原始和近端干细胞是人类肝脏的主要干细胞。 人原始肝干细胞可以通过人肝脏的免疫选择或在选择人原始肝干细胞的条件下培养人肝细胞来分离。 可以通过免疫选择或通过在包括发育因子的条件下培养人肝细胞来分离近端肝干细胞。 也可以通过在包括发育因子的条件下培养包含原始肝干细胞的菌落来分离近端肝干细胞。 所得组合物可用于治疗肝脏疾病和用于生产生物人造器官。