公开(公告)号：US08618024B2

公开(公告)日：2013-12-31

申请号：US11514850

申请日：2006-08-31

Applicant: Yen Choo ,  Aaron Klug ,  Mark Isalan

Inventor： Yen Choo ,  Aaron Klug ,  Mark Isalan

IPC: C40B50/04 ,  C07K1/00

CPC classification number: C40B40/02 ,  C07K1/047 ,  C07K14/4702 ,  C12N15/1037

Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.

Abstract translation: 本发明涉及一种锌指多肽文库，其中每个多肽包含至少部分随机化的多于一个锌指，以及编码重叠锌指多肽的一组锌指多肽文库，每个多肽包含多于一个锌 手指至少部分随机化，并且哪些多肽可以在选择后组装以形成多角蛋白锌指多肽。

公开(公告)号：US20070077227A1

公开(公告)日：2007-04-05

申请号：US11486962

申请日：2006-07-14

Applicant: Yen Choo ,  Aaron Klug ,  Mark Isalan

Inventor： Yen Choo ,  Aaron Klug ,  Mark Isalan

IPC: A61K48/00 ,  C12Q1/68 ,  G01N33/53 ,  C07H21/04 ,  C12P21/06 ,  C12N9/22 ,  C12N15/86

CPC classification number: C40B40/02 ,  C07K1/047 ,  C07K14/4702 ,  C12N15/1037

Abstract: The invention provides a method for designing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an alpha-helical zinc finger nucleic acid binding motif in the protein is determined as follows: if base 4 in the quadruplet is A, then position +6 in the alpha-helix is Glu, Asn or Val; if base 4 in the quadruplet is C, then position +6 in the alpha-helix is Ser, Thr, Val, Ala, Glu or Asn; if base 4 of the quadruplet is G, the position +6 in the alpha helix is Arg or Lys; if position 4 in the quadruplet is T, then position +6 in the alpha helix is Ser, Thr, Val or Lys.

Abstract translation: 本发明提供了一种用于设计能够与靶核酸序列中的核酸四联体结合的Cys2-His2锌指类的核酸结合蛋白的方法，其中通过α-螺旋锌指结合四联体的碱基4 蛋白质中的核酸结合基序如下测定：如果四联体中的碱基为A，则α-螺旋中的位置+6为Glu，Asn或Val; 如果四联体中的碱基为C，则α-螺旋中的位置+6是Ser，Thr，Val，Ala，Glu或Asn; 如果四联体的碱基4是G，则α螺旋中的位置+6是Arg或Lys; 如果四联体中的位置4是T，则α螺旋中的位置+6是Ser，Thr，Val或Lys。

公开(公告)号：US08617807B2

公开(公告)日：2013-12-31

申请号：US11486962

申请日：2006-07-14

Applicant: Yen Choo ,  Aaron Klug ,  Mark Isalan

Inventor： Yen Choo ,  Aaron Klug ,  Mark Isalan

IPC: C12Q1/68

CPC classification number: C40B40/02 ,  C07K1/047 ,  C07K14/4702 ,  C12N15/1037

Abstract: The invention provides a method for designing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an alpha-helical zinc finger nucleic acid binding motif in the protein is determined as follows: if base 4 in the quadruplet is A, then position +6 in the alpha-helix is Glu, Asn or Val; if base 4 in the quadruplet is C, then position +6 in the alpha-helix is Ser, Thr, Val, Ala, Glu or Asn; if base 4 of the quadruplet is G, the position +6 in the alpha helix is Arg or Lys; if position 4 in the quadruplet is T, then position +6 in the alpha helix is Ser, Thr, Val or Lys.

Abstract translation: 本发明提供了一种用于设计能够与靶核酸序列中的核酸四联体结合的Cys2-His2锌指类的核酸结合蛋白的方法，其中通过α-螺旋锌指结合四联体的碱基4 蛋白质中的核酸结合基序如下测定：如果四联体中的碱基为A，则α-螺旋中的位置+6为Glu，Asn或Val; 如果四联体中的碱基为C，则α-螺旋中的位置+6是Ser，Thr，Val，Ala，Glu或Asn; 如果四联体的碱基4是G，则α螺旋中的位置+6是Arg或Lys; 如果四联体中的位置4是T，则α螺旋中的位置+6是Ser，Thr，Val或Lys。

公开(公告)号：US20050037385A1

公开(公告)日：2005-02-17

申请号：US10832735

申请日：2004-04-26

Applicant: Yen Choo ,  Aaron Klug ,  Mark Isalan

Inventor： Yen Choo ,  Aaron Klug ,  Mark Isalan

IPC: C12N15/09 ,  C07K1/04 ,  C07K14/47 ,  C12N1/21 ,  C12N15/10 ,  C12P21/02 ,  C12Q1/68 ,  C40B40/02 ,  C07H21/04 ,  C12N9/22 ,  C12P19/34

CPC classification number: C40B40/02 ,  C07K1/047 ,  C07K14/4702 ,  C12N15/1037

Abstract: Disclosed herein are methods for designing DNA binding proteins comprising a plurality of zinc fingers and methods for binding the proteins to target nucleotide sequences in cells.

Abstract translation: 本文公开了用于设计包含多个锌指的DNA结合蛋白的方法和将蛋白质与细胞中的靶核苷酸序列结合的方法。

公开(公告)号：US06492117B1

公开(公告)日：2002-12-10

申请号：US09614679

申请日：2000-07-12

Applicant: Yen Choo ,  Mark Isalan ,  Sachin D. Patel ,  Shhankar Balasubramanian ,  Xiaohai Liu

Inventor： Yen Choo ,  Mark Isalan ,  Sachin D. Patel ,  Shhankar Balasubramanian ,  Xiaohai Liu

IPC: C12Q168

CPC classification number: A61K38/1709 ,  C07K14/4702 ,  C07K2319/00

Abstract: The present invention relates to isolated or purified molecule(s) capable of binding to one or more of telomeric, G-quadruplex, or G-quartet nucleic acid(s).

Abstract translation: 本发明涉及能够结合一种或多种端粒，G-四链体或G-四重核酸的分离或纯化的分子。

公开(公告)号：US07947469B2

公开(公告)日：2011-05-24

申请号：US10470065

申请日：2002-01-22

Applicant: Michael Moore ,  Mark Isalan ,  Lindsey Reynolds ,  Christopher Ullman ,  John Girdlestone ,  Christophe Demaison ,  Yen Choo

Inventor： Michael Moore ,  Mark Isalan ,  Lindsey Reynolds ,  Christopher Ullman ,  John Girdlestone ,  Christophe Demaison ,  Yen Choo

IPC: C12P21/04 ,  C12P21/06 ,  C12Q1/68 ,  C12N5/00 ,  C07H21/04

CPC classification number: C07K14/4703 ,  A61K38/00

Abstract: Disclosed herein are methods and compositions for modulating HIV infection by regulating expression of one or more receptors.

Abstract translation: 本文公开了通过调节一种或多种受体的表达来调节HIV感染的方法和组合物。

公开(公告)号：US07759059B2

公开(公告)日：2010-07-20

申请号：US11515369

申请日：2006-08-31

Applicant: Yen Choo ,  Aaron Klug ,  Mark Isalan

Inventor： Yen Choo ,  Aaron Klug ,  Mark Isalan

IPC: C12Q1/68

CPC classification number: C40B40/02 ,  C07K1/047 ,  C07K14/4702 ,  C12N15/1037

Abstract: The application provides methods of designing zinc finger binding polypeptides for binding to particular target sequences comprising overlapping nucleotide quadruplets.

Abstract translation: 本申请提供了设计锌指结合多肽以结合包含重叠核苷酸四联体的特定靶序列的方法。

公开(公告)号：US20070161014A1

公开(公告)日：2007-07-12

申请号：US11515369

申请日：2006-08-31

Applicant: Yen Choo ,  Aaron Klug ,  Mark Isalan

Inventor： Yen Choo ,  Aaron Klug ,  Mark Isalan

IPC: C12Q1/68 ,  G06F19/00 ,  C07H21/04 ,  C12P21/06 ,  C12P19/34 ,  C12N9/22

CPC classification number: C40B40/02 ,  C07K1/047 ,  C07K14/4702 ,  C12N15/1037

Abstract: The application provides methods of designing zinc finger binding polypeptides for binding to particular target sequences comprising overlapping nucleotide quadruplets.

Abstract translation: 本申请提供了设计锌指结合多肽以结合包含重叠核苷酸四联体的特定靶序列的方法。

公开(公告)号：US20070009962A1

公开(公告)日：2007-01-11

申请号：US11514850

申请日：2006-08-31

Applicant: Yen Choo ,  Aaron Klug ,  Mark Isalan

Inventor： Yen Choo ,  Aaron Klug ,  Mark Isalan

IPC: C40B40/10

CPC classification number: C40B40/02 ,  C07K1/047 ,  C07K14/4702 ,  C12N15/1037

Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.

公开(公告)号：US06977154B1

公开(公告)日：2005-12-20

申请号：US09646353

申请日：1999-03-17

Applicant: Yen Choo ,  Mark Isalan

Inventor： Yen Choo ,  Mark Isalan

IPC: C12N15/09 ,  C07K14/00 ,  C07K14/47 ,  C12N9/22 ,  C12N15/10 ,  C12P21/02 ,  C40B40/02 ,  G01N33/53

CPC classification number: C40B40/02 ,  C07K14/4702 ,  C07K2319/00 ,  C12N9/22 ,  C12N15/1037

Abstract: The invention provides a method for producing a zinc finger polypeptide which binds to a target nucleic acid sequence containing a modified base but not to an identical sequence containing an equivalent unmodified base.

Abstract translation: 本发明提供了一种制备锌指多肽的方法，所述锌指多肽与含有经修饰的碱基但不含有相当未改性碱基的相同序列的靶核酸序列结合。