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公开(公告)号：US20070111234A1
公开(公告)日：2007-05-17
申请号：US11514777
申请日：2006-09-01
CPC分类号： C12Q1/6806 , C12Q2565/627 , C12Q2561/113
摘要： The present invention provides a method, a kit and a system for the analysis of samples with respect to the presence of biological DNA based on real-time polymerase chain reaction (PCR) in combination with an analyzing step. The method comprises the steps of performing a universal real-time PCR to amplify biological DNA in the sample, and analyzing the amplified biological DNA using a mass spectrometer (MS).
摘要翻译： 本发明提供了一种与分析步骤相结合的基于实时聚合酶链式反应（PCR）的生物DNA存在的样品分析方法，试剂盒和系统。 该方法包括以下步骤：进行通用实时PCR以扩增样品中的生物DNA，并使用质谱仪（MS）分析扩增的生物DNA。
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公开(公告)号：US20070141563A1
公开(公告)日：2007-06-21
申请号：US11638655
申请日：2006-12-12
申请人： Thomas Emrich , Gerd Haberhausen , Martin Moczko
发明人： Thomas Emrich , Gerd Haberhausen , Martin Moczko
CPC分类号： C12Q1/6844 , C12Q2600/166 , C12Q2545/101
摘要： The present invention is related to a method for detecting a target biomolecule in test sample by adding an internal control biomolecule to the test sample; to a negative control sample, to a positive control sample and to a reagent control sample or adding an internal control biomolecule to the test sample, to a negative control sample, to a positive control sample comprising the target biomolecule and providing a reagent control sample comprising the target biomolecule, determining in each sample a signal, and verifying the signal thereby detecting the target biomolecule. The invention is also related to a method for verifying the determination of a signal indicating the presence of a target biomolecule. The invention is further related to a method for detecting the presence or the absence of a member of a group of target nucleic acids in a sample and a method for verifying the determination of a signal indicating the presence of a member of a group of target nucleic acids.
摘要翻译： 本发明涉及通过向测试样品添加内部控制生物分子来检测测试样品中的靶生物分子的方法; 将阴性对照样品与阳性对照样品和试剂对照样品或者将测试样品的内部对照生物分子加入到阴性对照样品中，加入到包含目标生物分子的阳性对照样品中，并提供试剂对照样品，其包含 目标生物分子，在每个样品中确定一个信号，并验证信号从而检测目标生物分子。 本发明还涉及用于验证指示存在目标生物分子的信号的确定的方法。 本发明还涉及用于检测样品中一组靶核酸成员的存在或不存在的方法以及用于验证指示靶核酸组成员的信号的确定的方法 酸。
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公开(公告)号：US07981606B2
公开(公告)日：2011-07-19
申请号：US11638655
申请日：2006-12-12
申请人： Thomas Emrich , Gerd Haberhausen , Martin Moczko
发明人： Thomas Emrich , Gerd Haberhausen , Martin Moczko
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2600/166 , C12Q2545/101
摘要： The present invention is related to a method for detecting a target biomolecule in test sample by adding an internal control biomolecule to the test sample; to a negative control sample, to a positive control sample and to a reagent control sample or adding an internal control biomolecule to the test sample, to a negative control sample, to a positive control sample comprising the target biomolecule and providing a reagent control sample comprising the target biomolecule, determining in each sample a signal, and verifying the signal thereby detecting the target biomolecule. The invention is also related to a method for verifying the determination of a signal indicating the presence of a target biomolecule. The invention is further related to a method for detecting the presence or the absence of a member of a group of target nucleic acids in a sample and a method for verifying the determination of a signal indicating the presence of a member of a group of target nucleic acids.
摘要翻译： 本发明涉及通过向测试样品添加内部控制生物分子来检测测试样品中的靶生物分子的方法; 将阴性对照样品与阳性对照样品和试剂对照样品或者将测试样品的内部对照生物分子加入到阴性对照样品中，加入到包含目标生物分子的阳性对照样品中，并提供试剂对照样品，其包含 目标生物分子，在每个样品中确定一个信号，并验证信号从而检测目标生物分子。 本发明还涉及用于验证指示存在目标生物分子的信号的确定的方法。 本发明还涉及用于检测样品中一组靶核酸成员的存在或不存在的方法以及用于验证指示靶核酸组成员的信号的确定的方法 酸。
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公开(公告)号：US20060099596A1
公开(公告)日：2006-05-11
申请号：US10534915
申请日：2003-12-02
CPC分类号： C12Q1/689 , C12Q1/6851
摘要： The present invention is directed to a method for identification of a pathogenic organism from a predetermined group of pathogens, comprising (i) isolating a clinical sample containing at least partially purified nucleic acid, (ii) subjecting at least a first aliquot of said clinical specimen to at least one amplification and detection reaction in one reaction vessel comprising (iia) an amplification step using at least a first set of amplification primers capable of amplifying a preselected nucleic acid sequence region from several or all members of said predetermined group of pathogens, (iib) a detection step using at least 2, 3 or multiple hybridization reagents, said reagents together being capable of specifically detecting a pre-selected nucleic acid sequence region from all members of said group of pathogens, said detection step (iib) comprising steps monitoring hybridization of each of said hybridization reagents at a pre-selected temperature, said hybridization being indicative for at least the genus of said pathogen present in the sample, and monitoring temperature dependence of hybridization, said temperature dependence being indicative for at least the species of said pathogen, and (iii) determining whether said amplification and detection reaction is indicative for the presence of a specific member of said pre-selected group of pathogens.
摘要翻译： 本发明涉及从预定病原体组中鉴定病原生物的方法，其包括（i）分离含有至少部分纯化的核酸的临床样品，（ii）对所述临床标本的至少第一等分试样 至少在一个反应容器中进行扩增和检测反应，其包括（i）扩增步骤，使用至少第一组扩增引物，所述第一组扩增引物能够扩增来自所述预定病原体组的数个或全部成员的预选核酸序列区（ iib）使用至少2,3或多个杂交试剂的检测步骤，所述试剂一起能够特异性检测来自所述病原体组的所有成员的预先选择的核酸序列区，所述检测步骤（iib）包括监测步骤 每个所述杂交试剂在预选温度下进行杂交，所述杂交是指示性的 至少存在于样品中的所述病原体的属和监测杂交的温度依赖性，所述温度依赖性指示至少所述病原体的种类，和（iii）确定所述扩增和检测反应是否表明存在 的所述预先选择的病原体组的特定成员。
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