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    SET OF OLIGONUCLEOTIDE PROBES AS WELL AS METHODS AND USES THERETO
    1.
    发明申请
    SET OF OLIGONUCLEOTIDE PROBES AS WELL AS METHODS AND USES THERETO 有权
    作为方法和用途的一组寡核苷酸探针

    公开(公告)号：US20120164643A1

    公开(公告)日：2012-06-28

    申请号：US13361549

    申请日：2012-01-30

    申请人： Frank Bergmann ,  Walter Eberle ,  Thomas Fischer ,  Herbert von der Eltz

    发明人： Frank Bergmann ,  Walter Eberle ,  Thomas Fischer ,  Herbert von der Eltz

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6841 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q2565/1025 ,  C12Q2563/107

    摘要： The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.

    摘要翻译： 本公开内容涉及针对感兴趣的基因组靶序列的（或互补）部分的一组至少100个单链寡核苷酸探针。 本公开还涉及使用该组寡核苷酸探针检测感兴趣的基因组靶序列的方法和产生寡核苷酸探针组的方法。 此外，本公开涉及包含该组寡核苷酸探针和至少一种另外的组分的试剂盒。

    Method for bisulfite treatment
    3.
    发明授权
    Method for bisulfite treatment 失效
    亚硫酸氢盐处理方法

    公开(公告)号：US07501240B2

    公开(公告)日：2009-03-10

    申请号：US10573215

    申请日：2004-12-01

    申请人： Christian Birkner ,  Christine Markert-Hahn ,  Herbert von der Eltz

    发明人： Christian Birkner ,  Christine Markert-Hahn ,  Herbert von der Eltz

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C12Q1/6827 ,  C12Q2527/125 ,  C12Q2523/125

    摘要： The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.

    摘要翻译： 本发明涉及检测核酸中的甲基化胞嘧啶，其中将亚硫酸氢胍用于制备含有胍离子和亚硫酸根离子的溶液以及随后的核酸修饰。 由此，将非甲基化胞嘧啶转化为尿嘧啶。 本发明还公开了用于执行本发明方法的套件。

    Nucleotides labelled with an infrared dye and their use in nucleic acid detection
    4.
    发明授权
    Nucleotides labelled with an infrared dye and their use in nucleic acid detection 失效
    用红外染料标记的核苷酸及其在核酸检测中的用途

    公开(公告)号：US06573374B1

    公开(公告)日：2003-06-03

    申请号：US08411761

    申请日：1995-03-28

    申请人： Klaus Muehlegger ,  Hans-Joachim Hoeltke ,  Christian Birkner ,  Herbert Von Der Eltz

    发明人： Klaus Muehlegger ,  Hans-Joachim Hoeltke ,  Christian Birkner ,  Herbert Von Der Eltz

    IPC分类号： C07H1904

    CPC分类号： C07H19/10 ,  C07F9/5728 ,  C07H19/20 ,  C07H21/00

    摘要： Nucleoside-5′-triphosphates and phosphoramidites which carry a residue absorbing in the long wavelength region, preferably a carbocyanine group of the general formula (I), on the base portion or on the phosphorus atom in which R1 and R2 each denote hydrogen or together form a phenyl residue; R3 denotes hydrogen if linkage with the nucleotide is via the R4 position or it denotes a —NHCS— group if linkage with the nucleotide is via the R3 position; both R4 and R5, or R5, alone denote an alkylsulfonyl group with n being a number from 3 to 5 or R4 represents a —NHCS— group with n being a number from 3 to 8, as well as the use of the compounds to label, detect and sequence nucleic acids.

    摘要翻译： 核苷-5'-三磷酸和亚磷酰胺，其在碱性部分或磷原子上具有在长波长区域吸收的残基，优选为通式（I）的碳菁基团，其中R1和R2各自表示氢或一起形成 苯基残基; 如果与核苷酸的连接通过R4位置，R3表示氢，如果与核苷酸的连接通过R3位置，则表示-NHCS-基团; R4和R5或R5独立地表示烷基磺酰基，n为3至5的数，或R4表示-NHCS-基，n为3至8的数，以及该化合物用于标记 ，检测和序列核酸。

    Heterocyclic compounds and their use for isolating nucleic acids
    5.
    发明授权
    Heterocyclic compounds and their use for isolating nucleic acids 有权
    杂环化合物及其分离核酸的用途

    公开(公告)号：US06403786B1

    公开(公告)日：2002-06-11

    申请号：US09254644

    申请日：1999-07-02

    申请人： Klaus Mühlegger ,  Herbert Von der Eltz

    发明人： Klaus Mühlegger ,  Herbert Von der Eltz

    IPC分类号： C07H1910

    CPC分类号： C07D405/04 ,  C07D413/04 ,  C07F9/65586 ,  C07H7/06 ,  C07H19/04 ,  C07H19/048 ,  C07H19/06 ,  C07J51/00

    摘要： The invention concerns compounds of the general formula (I) in which i:he residues R1 to R7 have the meanings given in the application as well as methods for their preparation. The compounds are in particular suitable as substrates for RNA or DNA polymerases and can thus be incorporated into RNA or DNA oligonucleotides and are especially suitable for labelling and detecting nucleic acids or for DNA sequencing.

    摘要翻译： 本发明涉及通式（I）的化合物，其中I：残基R1至R7具有本申请中给出的含义及其制备方法。 该化合物特别适合作为RNA或DNA聚合酶的底物，因此可以并入RNA或DNA寡核苷酸，特别适用于标记和检测核酸或进行DNA测序。

    Nucleosides, nucleotides and oligonucleotides containing enzymatically cleavable protecting groups
    9.
    发明授权
    Nucleosides, nucleotides and oligonucleotides containing enzymatically cleavable protecting groups 失效
    含有酶切割保护基团的核苷，核苷酸和寡核苷酸

    公开(公告)号：US5677441A

    公开(公告)日：1997-10-14

    申请号：US326490

    申请日：1994-10-20

    申请人： Herbert Waldman ,  Armin Reidel ,  Axel Heuser ,  Klaus Muehlegger ,  Herbert Von Der Eltz ,  Christian Birkner

    发明人： Herbert Waldman ,  Armin Reidel ,  Axel Heuser ,  Klaus Muehlegger ,  Herbert Von Der Eltz ,  Christian Birkner

    IPC分类号： C12Q1/68 ,  C07H19/04 ,  C07H19/073 ,  C07H19/173 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/70 ,  C07H19/06 ,  C07H19/16

    CPC分类号： C07H21/00 ,  C07H19/04 ,  Y02P20/55

    摘要： Process for the production of oligonucleotides of formula II, in which the exocyclic amino groups of the bases adenine, guanine, cytosine, 7-deazaadenine and 7-deazaguanine carry N-phenylacetyl groups, are used for oligonucleotide synthesis, wherein in a first step a starting nucleotide is bound to a solid carrier, subsequently the desired oligonucleotide is synthesized by stepwise coupling with appropriately activated further monomeric nucleotide building blocks of the general formula I with the above-mentioned meanings, if desired, trivalent phosphorus is oxidized to pentavalent phosphorus during and after the synthesis, the oligonucleotide is cleaved from the carrier and the 5' protecting groups are cleaved off. The phenylacetyl functional groups that protect exocyclic NH.sub.2 groups of the bases can be cleaved off in a mild way with penicillin amidohydrolase (EC 3.5.1.11).

    摘要翻译： 用于生产式II寡核苷酸的方法，其中碱基腺嘌呤，鸟嘌呤，胞嘧啶，7-脱氮鸟嘌呤和7-脱氮鸟嘌呤的环外氨基携带N-苯乙酰基用于寡核苷酸合成，其中在第一步中 起始核苷酸与固体载体结合，随后所需的寡核苷酸通过与适当活化的具有上述含义的通式I的其它单体核苷酸结构单元逐步偶联来合成，如果需要，三价磷被氧化成五价磷， 合成后，将寡核苷酸从载体上切下，5'保护基被切掉。 保护碱基的环外NH2基团的苯乙酰基官能团可以用青霉素酰胺水解酶（EC 3.5.1.11）以温和的方式裂解。

    Cyclodextrin-biocide complex
    10.
    发明授权
    Cyclodextrin-biocide complex 失效
    环糊精 - 杀菌剂复合物

    公开(公告)号：US5506216A

    公开(公告)日：1996-04-09

    申请号：US231170

    申请日：1994-04-22

    申请人： Axel Schmidt ,  Herbert von der Eltz ,  Klaus Kaluza

    发明人： Axel Schmidt ,  Herbert von der Eltz ,  Klaus Kaluza

    IPC分类号： A61K9/08 ,  A01N25/10 ,  A01N25/22 ,  A01N31/08 ,  A01N47/48 ,  A61K47/40 ,  C08B37/00 ,  G01N33/52 ,  C08B37/16 ,  C09K15/04 ,  C12Q1/00 ,  C07K15/06

    CPC分类号： A01N25/10 ,  C08B37/0015 ,  G01N33/52

    摘要： The invention concerns a complex of cyclodextrin and an organic biocide, wherein the organic biocide has a maximum water-solubility of 0.15% (w/v) at a temperature of 25.degree. C., a process for the production of the complex as well as the use of the complex for preserving aqueous solutions. In addition the invention concerns a diagnostic test solution which contains at least one complex according to the invention and a preserved diagnostic test kit which comprises test reagents and at least one complex according to the invention.

    摘要翻译： 本发明涉及环糊精和有机杀生物剂的复合物，其中有机杀生物剂在25℃的温度下具有0.15％（w / v）的最大水溶解度，生产复合物的方法以及 使用复合物保存水溶液。 此外，本发明涉及包含至少一种根据本发明的复合物的诊断测试溶液和包含测试试剂和至少一种根据本发明的复合物的保存的诊断测试试剂盒。