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公开(公告)号：US4016147A
公开(公告)日：1977-04-05
申请号：US649794
申请日：1976-01-16
申请人： Masao Fujimaki , Soichi Arai , Michiko Watanabe
发明人： Masao Fujimaki , Soichi Arai , Michiko Watanabe
CPC分类号： A23J3/34 , Y10S435/803 , Y10S530/857
摘要： Method for the preparation of a low-phenylalanine plastein comprising hydrolyzing stepwise a certain albumin or globulin protein with an endopeptidase having an affinity for the aromatic acid components, and an exopeptidase, subjecting the hydrolyzate thus-obtained to gel-filtration to divide it into two fractions, one being constituted with aromatic amino acids and the other being constituted with oligopeptides almost free from the aromatic amino acids, and then subjecting the latter fraction to usual plastein synthesis. Tyrosine and/or tryptophan may be added to the substrate at the plastein synthesis in order to incorporate them into the plastein molecules. The resultant plasteins may be purified through ultra-filtration in order to remove the remaining free amino acids and low-molecular peptides.
摘要翻译： 制备低苯丙氨酸Plastein的方法，包括用对芳族酸组分具有亲和力的内肽酶逐步水解某种白蛋白或球蛋白蛋白，以及外肽酶，使由此得到的水解产物进行凝胶过滤，将其分成两部分 一部分由芳族氨基酸构成，另一个由几乎不含芳香族氨基酸的寡肽构成，然后使后一部分进行常规的Plastein合成。 酪氨酸和/或色氨酸可以在Plastein合成中加入到底物中，以便将它们掺入Plastein分子中。 所得到的糊精可以通过超滤来纯化，以除去剩余的游离氨基酸和低分子肽。
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公开(公告)号：US4145455A
公开(公告)日：1979-03-20
申请号：US673885
申请日：1976-04-05
IPC分类号： C12P21/02 , A23C20/00 , A23J3/00 , A23J3/34 , A23L15/00 , C07K1/107 , C07K1/113 , C08H1/00 , C08L89/00 , G03C1/047 , A23J3/02
摘要： A modified protein composition which is produced by contacting a water-soluble protein, such as plant seed proteins, cow milk protein, animal meat proteins, fish meat proteins, egg proteins and microorganisms proteins, with a cysteine-enriched plastein in an amount of at least 1% by weight on the basis of the water-soluble protein in an aqueous medium, and if desired, heating the resultant under a mild condition (e.g. at a temperature of 40.degree. to 90.degree. C.), said cysteine-enriched plastein being produced by hydrolyzing a protein with an enzyme having an endopeptidase activity and subjecting the hydrolyzate to dehydration-condensation with a protease having an esterase activity in the presence of an activated cysteine (e.g. lower alkyl cysteinate, N-acetyl-L-cysteine or L-cysteinyl-L-cysteine).
摘要翻译： 通过将水溶性蛋白质（例如植物种子蛋白质，牛奶蛋白质，动物肉蛋白质，鱼肉蛋白质，蛋类蛋白质和微生物蛋白质）与富含半胱氨酸的Plastein接触的修饰蛋白质组合物，其量为 在水性介质中基于水溶性蛋白质至少为1重量％，如果需要，在温和条件（例如在40℃至90℃的温度下）加热所得的所述富含半胱氨酸的Plastein 通过用具有内肽酶活性的酶水解蛋白质而产生，并且在活化的半胱氨酸（例如低级烷基半胱氨酸，N-乙酰基-L-半胱氨酸或L）的存在下，使水解产物与具有酯酶活性的蛋白酶脱水缩合 - 半胱氨酰-L-半胱氨酸）。
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