公开(公告)号：US07977057B2

公开(公告)日：2011-07-12

申请号：US12771897

申请日：2010-04-30

申请人： Matthew Beaudet ,  Gregory Cox ,  Stephen Yue

发明人： Matthew Beaudet ,  Gregory Cox ,  Stephen Yue

IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07D209/04

CPC分类号： G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

摘要翻译： 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。 通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。 优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。 使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

公开(公告)号：US20050239096A1

公开(公告)日：2005-10-27

申请号：US10956868

申请日：2004-09-30

申请人： Matthew Beaudet ,  W. Cox ,  Stephen Yue

发明人： Matthew Beaudet ,  W. Cox ,  Stephen Yue

IPC分类号： C07D413/02 ,  C07D417/02 ,  C07D417/06 ,  C09B23/02 ,  C12Q1/68 ,  C07D43/02

CPC分类号： G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

摘要翻译： 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。 通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。 优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。 使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

公开(公告)号：US08562802B1

公开(公告)日：2013-10-22

申请号：US13213953

申请日：2011-08-19

申请人： Matthew Beaudet ,  Ilana Margalit ,  Ronen Benarieh ,  Andres Wainstein ,  Gustavo Turkieltaub

发明人： Matthew Beaudet ,  Ilana Margalit ,  Ronen Benarieh ,  Andres Wainstein ,  Gustavo Turkieltaub

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： G01N27/44704

摘要： Cassette electrophoresis systems that allow viewing of molecules during the electrophoresis run are disclosed. Cassette electrophoresis bases that reversibly engage light sources, such as light source bases are disclosed. Also disclosed are visible light transillumination systems for viewing a pattern of fluorescence emitted by fluorophores comprising a cassette housing fluorophore-containing material and a base unit to support the cassette. In some aspects the base unit that includes a power supply also houses a light source having output in the visible wavelength region and a filter placed between the light source and the fluorophores. The system is constructed and arranged such that patterns of fluorescence emitted by the fluorophores are viewable. Also described are charging devices for providing charge to gel electrophoresis systems, portable gel electrophoresis systems and methods of use thereof.

摘要翻译： 公开了允许在电泳过程中观察分子的盒式电泳系统。 公开了可逆地接合诸如光源基底之类的光源的盒式电泳基座。 还公开了可见光透照系统，用于观察由荧光团发射的荧光图案，所述荧光团包括含有含荧光团的材料的盒和用于支撑所述盒的基体单元。 在一些方面，包括电源的基本单元还容纳在可见光波长区域具有输出的光源，以及放置在光源和荧光团之间的滤光器。 该系统的构造和布置使得荧光团发出的荧光图案是可见的。 还描述了用于向凝胶电泳系统提供电荷的充电装置，便携式凝胶电泳系统及其使用方法。