公开(公告)号：USD1072272S1

公开(公告)日：2025-04-22

申请号：US29790514

申请日：2021-12-01

Applicant: MaxCyte, Inc.

Designer： James William Luther ,  Bertold Engler ,  Andrea Besana ,  Thomas Alan Peach

公开(公告)号：USD1066718S1

公开(公告)日：2025-03-11

申请号：US29788796

申请日：2021-07-28

Applicant: MaxCyte, Inc.

Designer： Frank Modica ,  James William Luther ,  Bertold Engler ,  Andrea Besana ,  Thomas Alan Peach

公开(公告)号：US11608511B2

公开(公告)日：2023-03-21

申请号：US15566320

申请日：2016-04-13

Applicant: MaxCyte, Inc.

Inventor： Linhong Li ,  Madhusudan Peshwa

IPC: C12N15/90 ,  A61K48/00 ,  C12N5/0783 ,  A61P35/00 ,  A61K35/17 ,  A61K35/33 ,  C12N5/077 ,  C12N9/22 ,  C12N9/96 ,  C12N15/11

Abstract: Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: contacting the cells with an activating composition; transfecting the cells with a transfection composition comprising (a) donor DNA and (b) a DNA digesting agent; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region; and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.

公开(公告)号：US10660917B2

公开(公告)日：2020-05-26

申请号：US15602536

申请日：2017-05-23

Applicant: Maxcyte, Inc.

Inventor： Linhong Li ,  Madhusudan V. Peshwa

IPC: A61K35/17 ,  A61K39/00 ,  C12N5/078 ,  C12N5/0783 ,  A61K39/395 ,  C12N13/00 ,  C12N15/85 ,  A61K35/12

Abstract: The present invention relates to the transient modification of cells. In particular embodiments, the cells are immune systems, such as PBMC, PBL, T (CD3+ and/or CD8+) and Natural Killer (NK) cells. The modified cells provide a population of cells that express a genetically engineered chimeric receptor which can be administered to a patient therapeutically. The present invention further relates to methods that deliver mRNA coding for the chimeric receptor to unstimulated resting PBMC, PBL, T (CD3+ and/or CD8+) and NK cells and which delivers the mRNA efficiently to the transfected cells and promotes significant target cell killing.

公开(公告)号：US20190247436A1

公开(公告)日：2019-08-15

申请号：US16316721

申请日：2017-07-21

Applicant: MAXCYTE, INC.

Inventor： Linhong LI ,  Cornell ALLEN ,  Madhusudan PESHWA

IPC: A61K35/17 ,  C12N5/0783 ,  C12N5/0775 ,  C12N5/077 ,  C12N15/82 ,  A01N1/02 ,  A61K35/33

CPC classification number: A61K35/17 ,  A01N1/0221 ,  A61K35/33 ,  A61K48/00 ,  A61K48/0016 ,  A61K48/005 ,  C12N5/0636 ,  C12N5/0656 ,  C12N5/0663 ,  C12N15/8206 ,  C12N15/87 ,  C12N15/907

Abstract: Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: transfecting the cells by electroporation with a composition comprising (a) a DNA oligo; (b) a DNA digesting agent; and (c) a targeting RNA, wherein the targeting RNA is capped and/or polyadenylated; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.

公开(公告)号：US20180112235A1

公开(公告)日：2018-04-26

申请号：US15566320

申请日：2016-04-13

Applicant: MaxCyte, Inc.

Inventor： Linhong Li ,  Madhusudan PESHWA

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22 ,  C12N9/96 ,  C12N5/0783 ,  C12N5/077 ,  A61K35/17 ,  A61K35/33 ,  A61P35/00

CPC classification number: C12N15/907 ,  A61K35/17 ,  A61K35/33 ,  A61K48/00 ,  A61P35/00 ,  C12N5/0636 ,  C12N5/0646 ,  C12N5/0652 ,  C12N9/22 ,  C12N9/96 ,  C12N15/11 ,  C12N15/902 ,  C12N2510/00

Abstract: Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: contacting the cells with an activating composition; transfecting the cells with a transfection composition comprising (a) donor DNA and (b) a DNA digesting agent; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region; and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.

公开(公告)号：US20040115784A1

公开(公告)日：2004-06-17

申请号：US10675592

申请日：2003-09-30

Applicant: Maxcyte, Inc.

Inventor： Sergey Dzekunov

IPC: C12N013/00 ,  C12M001/42

CPC classification number: C12N15/87 ,  C12M35/02

Abstract: Techniques for streaming electroporation. A representative but non-limiting method includes: generating a spatially inhomogeneous electric field with a pair of electrodes and displacing the pair of electrodes and a sample relative to one other while the electric field is substantially constant in terms of magnitude so that the sample is displaced across electric field lines for a time sufficient to effect electroporation.

Abstract translation: 流式电穿孔技术。 一个代表性但非限制性的方法包括：在电场在幅度上基本上恒定的情况下，使用一对电极产生空间不均匀的电场并相对于彼此移动一对电极和样品，使得样品移位 穿过电场线路一段足以影响电穿孔的时间。

公开(公告)号：USD967126S1

公开(公告)日：2022-10-18

申请号：US29726271

申请日：2020-03-02

Applicant: MaxCyte, Inc.

Designer： James William Luther ,  Bertold Engler ,  Andrea Besana ,  Thomas Alan Peach

公开(公告)号：US11331344B2

公开(公告)日：2022-05-17

申请号：US15726740

申请日：2017-10-06

Applicant: Maxcyte, Inc.

Inventor： Linhong Li ,  Madhusudan V. Peshwa

IPC: A61K35/17 ,  A61K39/00 ,  C12N5/078 ,  C12N5/0783 ,  A61K39/395 ,  C12N13/00 ,  C12N15/85 ,  A61K35/12

Abstract: The present invention relates to the transient modification of cells. In particular embodiments, the cells are immune systems, such as PBMC, PBL, T (CD3+ and/or CD8+) and Natural Killer (NK) cells. The modified cells provide a population of cells that express a genetically engineered chimeric receptor which can be administered to a patient therapeutically. The present invention further relates to methods that deliver mRNA coding for the chimeric receptor to unstimulated resting PBMC, PBL, T (CD3+ and/or CD8+) and NK cells and which delivers the mRNA efficiently to the transfected cells and promotes significant target cell killing.

公开(公告)号：USD923200S1

公开(公告)日：2021-06-22

申请号：US29726262

申请日：2020-03-02

Applicant: MaxCyte, Inc.

Designer： James William Luther ,  Bertold Engler ,  Andrea Besana ,  Thomas Alan Peach