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    Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
    1.
    发明授权
    Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum 有权
    使用来自青霉菌的纤维二糖水解酶-1的超活性纤维素酶制剂

    公开(公告)号：US08283150B2

    公开(公告)日：2012-10-09

    申请号：US12247594

    申请日：2008-10-08

    申请人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    发明人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    IPC分类号： C07K1/00 ,  C07K14/00 ,  C07K17/00 ,  C12N9/42 ,  A23L1/202

    CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01091

    摘要： Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

    摘要翻译： 与其他Cel7家族成员酶相比，来自青霉菌（Penicillium funiculosum）的纯化纤维二糖水解酶I（糖基水解酶家族7（Cel7A））酶表现出高水平的特异性，当用来自解纤维解卷菌的纯化的EIcd内切葡聚糖酶配制并在预处理的玉米秸秆上进行测试时。 纯化的天然酶以及重组表达的酶，例如在非天然曲霉属宿主中表达的酶的结果是真实的。 在具体实例中，与使用来自里氏木霉的纯化Cel7A的制剂相比，使用在泡盛曲霉中表达的来自青霉菌的纯化重组Cel7A的制剂的具体表现增加了超过200％。

    Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
    2.
    发明授权
    Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum 有权
    使用来自青霉菌的纤维二糖水解酶-1的超活性纤维素酶制剂

    公开(公告)号：US07449550B2

    公开(公告)日：2008-11-11

    申请号：US10557589

    申请日：2003-02-27

    申请人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    发明人： William S. Adney ,  John O. Baker ,  Stephen R. Decker ,  Yat-Chen Chou ,  Michael E. Himmel ,  Shi-You Ding

    IPC分类号： C07K14/00

    CPC分类号： C12N9/2437 ,  C12Y302/01004 ,  C12Y302/01091

    摘要： Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A) enzymes from Penicillium funiculosum demonstrate a high level of specific performance in comparison to other Cel7 family member enzymes when formulated with purified EIcd endoglucanase from A. cellulolyticus and tested on pretreated corn stover. This result is true of the purified native enzyme, as well as recombinantly expressed enzyme, for example, that enzyme expressed in a non-native Aspergillus host. In a specific example, the specific performance of the formulation using purified recombinant Cel7A from Penicillium funiculosum expressed in A. awamori is increased by more than 200% when compared to a formulation using purified Cel7A from Trichoderma reesei.

    摘要翻译： 与其他Cel7家族成员酶相比，纯化的纤维二糖水解酶I（糖基水解酶家族7（Cel7A））来自A. cellulolyticus的纯化EIcd内切葡聚糖酶，并与预处理的玉米秸秆进行了测试，结果表明： 纯化的天然酶，以及重组表达的酶，例如在非天然曲霉属宿主中表达的酶。在一个具体实例中，使用在A.A中表达的来自Penicillium funiculosum的纯化重组Cel7A的制剂的具体性能。 与使用来自里氏木霉的纯化Cel7A的制剂相比，泡盛曲增加超过200％。

    Cellobiohydrolase I enzymes
    8.
    发明授权
    Cellobiohydrolase I enzymes 有权
    纤维二糖水解酶I酶

    公开(公告)号：US08637293B2

    公开(公告)日：2014-01-28

    申请号：US12123352

    申请日：2008-05-19

    申请人： William S. Adney ,  Michael E. Himmel ,  Stephen R. Decker ,  Eric P. Knoshaug ,  Mark R. Nimlos ,  Michael F. Crowley ,  Tina Jeoh

    发明人： William S. Adney ,  Michael E. Himmel ,  Stephen R. Decker ,  Eric P. Knoshaug ,  Mark R. Nimlos ,  Michael F. Crowley ,  Tina Jeoh

    IPC分类号： C12N9/26 ,  C12N1/20 ,  C12N15/00 ,  C12P21/06 ,  C12P21/04 ,  C12Q1/00 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12Y302/01091 ,  C12N9/2437

    摘要： Provided herein is an isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide, wherein the mutations reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. Also provided herein is an isolated Cel7A polypeptide comprising increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The increased O-linked glycosylation is a result of the addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide. In some embodiments, the isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide further comprises increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The mutations in the catalytic domain reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. The addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide increases O-linked glycosylation of the isolated polypeptide. Further provided are compositions comprising such polypeptides and nucleic acids encoding such polypeptides. Still further provided are methods for making such polypeptides.

    摘要翻译： 本文提供了分离的Cel7A多肽，其包含相对于野生型Cel7A多肽的催化结构域的多肽的催化结构域中的突变，其中所述突变相对于野生型多肽减少分离的多肽的N-连接糖基化。 本文还提供了分离的Cel7A多肽，其包含相对于野生型Cel7A多肽的接头结构域的连接体结构域的O-连接糖基化。 增加的O-连接的糖基化是相对于野生型多肽的接头结构域向接头结构域添加和/或取代一个或多个丝氨酸和/或苏氨酸残基的结果。 在一些实施方案中，相对于野生型Cel7A多肽的催化结构域，包含多肽催化结构域中的突变的分离的Cel7A多肽进一步包含相对于野生型Cel7A多肽的接头结构域的连接体结构域的O-连接糖基化 。 催化结构域中的突变相对于野生型多肽减少分离的多肽的N-连接糖基化。 一个或多个丝氨酸和/或苏氨酸残基相对于野生型多肽的接头结构域添加和/或取代连接体结构域增加分离的多肽的O-连接的糖基化。 还提供了包含这样的多肽和编码这种多肽的核酸的组合物。 还提供了制备这种多肽的方法。

    CELLOBIOHYDROLASE I ENZYMES
    9.
    发明申请
    CELLOBIOHYDROLASE I ENZYMES 有权
    细胞脂肪酶I酶

    公开(公告)号：US20090162916A1

    公开(公告)日：2009-06-25

    申请号：US12123352

    申请日：2008-05-19

    申请人： William S. Adney ,  Michael E. Himmel ,  Stephen R. Decker ,  Eric P. Knoshaug ,  Mark R. Nimlos ,  Michael F. Crowley ,  Tina Jeoh

    发明人： William S. Adney ,  Michael E. Himmel ,  Stephen R. Decker ,  Eric P. Knoshaug ,  Mark R. Nimlos ,  Michael F. Crowley ,  Tina Jeoh

    IPC分类号： C12N9/42 ,  C07H21/04

    CPC分类号： C12Y302/01091 ,  C12N9/2437

    摘要： Provided herein is an isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide, wherein the mutations reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. Also provided herein is an isolated Cel7A polypeptide comprising increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The increased O-linked glycosylation is a result of the addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide. In some embodiments, the isolated Cel7A polypeptide comprising mutations in the catalytic domain of the polypeptide relative to the catalytic domain of a wild type Cel7A polypeptide further comprises increased O-linked glycosylation of the linker domain relative to a linker domain of a wild type Cel7A polypeptide. The mutations in the catalytic domain reduce N-linked glycosylation of the isolated polypeptide relative to the wild type polypeptide. The addition of and/or substitution of one or more serine and/or threonine residues to the linker domain relative to the linker domain of the wild type polypeptide increases O-linked glycosylation of the isolated polypeptide. Further provided are compositions comprising such polypeptides and nucleic acids encoding such polypeptides. Still further provided are methods for making such polypeptides.

    摘要翻译： 本文提供了分离的Cel7A多肽，其包含相对于野生型Cel7A多肽的催化结构域的多肽的催化结构域中的突变，其中所述突变相对于野生型多肽减少分离的多肽的N-连接糖基化。 本文还提供了分离的Cel7A多肽，其包含相对于野生型Cel7A多肽的接头结构域的连接体结构域的O-连接糖基化。 增加的O-连接的糖基化是相对于野生型多肽的接头结构域向接头结构域添加和/或取代一个或多个丝氨酸和/或苏氨酸残基的结果。 在一些实施方案中，相对于野生型Cel7A多肽的催化结构域，包含多肽催化结构域中的突变的分离的Cel7A多肽进一步包含相对于野生型Cel7A多肽的接头结构域的连接体结构域的O-连接糖基化 。 催化结构域中的突变相对于野生型多肽减少分离的多肽的N-连接糖基化。 一个或多个丝氨酸和/或苏氨酸残基相对于野生型多肽的接头结构域添加和/或取代连接体结构域增加分离的多肽的O-连接的糖基化。 还提供了包含这样的多肽和编码这种多肽的核酸的组合物。 还提供了制备这种多肽的方法。