利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

18 条记录 (耗时 0.03 秒)

    Transcription factor coactivator protein, p/CIP
    4.
    发明授权
    Transcription factor coactivator protein, p/CIP 失效
    转录因子辅激活蛋白，p / CIP

    公开(公告)号：US07211402B2

    公开(公告)日：2007-05-01

    申请号：US10971982

    申请日：2004-10-21

    申请人： Michael G. Rosenfeld ,  Christopher K. Glass ,  David W. Rose ,  Joseph Torchia

    发明人： Michael G. Rosenfeld ,  Christopher K. Glass ,  David W. Rose ,  Joseph Torchia

    IPC分类号： G01N33/53 ,  C07K14/47

    CPC分类号： C07K14/4702 ,  A61K38/00

    摘要： The present invention provides a substantially purified nucleic acid molecule encoding a p/CIP polypeptide, which regulates the activity of CBP/p300-dependent transcription factors. The invention also provides a substantially purified p-CIP polypeptide and active fragments thereof. In addition, the invention provides methods of identifying an effective agent that alters the association of a p/CIP polypeptide with a second protein. Further provided herein are methods of selectively inhibiting signal transduction pathways using an active fragment of a p/CIP polypeptide or a nucleic acid molecule encoding such an acive fragment.

    摘要翻译： 本发明提供了编码p / CIP多肽的基本上纯化的核酸分子，其调节CBP / p300依赖性转录因子的活性。 本发明还提供了基本上纯化的p-CIP多肽及其活性片段。 此外，本发明提供鉴定改变p / CIP多肽与第二蛋白质的缔合的有效试剂的方法。 本文进一步提供了使用p / CIP多肽的活性片段或编码这种酸性片段的核酸分子选择性抑制信号转导途径的方法。

    Transcription factor coactivator protein, p/CIP
    5.
    发明授权
    Transcription factor coactivator protein, p/CIP 失效
    转录因子辅激活蛋白，p / CIP

    公开(公告)号：US06812336B1

    公开(公告)日：2004-11-02

    申请号：US09445353

    申请日：2000-05-05

    申请人： Michael G. Rosenfeld ,  Christopher K. Glass ,  David W. Rose ,  Joseph Torchia

    发明人： Michael G. Rosenfeld ,  Christopher K. Glass ,  David W. Rose ,  Joseph Torchia

    IPC分类号： C07H2104

    CPC分类号： C07K14/4702 ,  A61K38/00

    摘要： The present invention provides a substantially purified nucleic acid molecule encoding p/CIP, which regulates the activity of CBP/p300 dependent transcription factors. The invention also provides substantially purified p/CIP and peptide portions of p/CIP. The invention also provides methods of selectively inhibiting signal transduction pathways using a peptide portion of p/CIP or a nucleic acid molecule encoding such a peptide portion.

    摘要翻译： 本发明提供了编码p / CIP的基本上纯化的核酸分子，其调节CBP / p300依赖性转录因子的活性。 本发明还提供p / CIP的基本纯化的p / CIP和肽部分。 本发明还提供了使用p / CIP的肽部分或编码这种肽部分的核酸分子选择性抑制信号转导途径的方法。

    Isolated DNA molecule encoding SPRM-1 protein
    6.
    发明授权
    Isolated DNA molecule encoding SPRM-1 protein 失效
    分离编码SPRM-1蛋白的DNA分子

    公开(公告)号：US5612220A

    公开(公告)日：1997-03-18

    申请号：US347826

    申请日：1994-12-01

    申请人： Bogi Andersen ,  Richard V. Pearse ,  Peter N. Schlegel ,  Michael G. Rosenfeld ,  C. Wayne Bardin

    发明人： Bogi Andersen ,  Richard V. Pearse ,  Peter N. Schlegel ,  Michael G. Rosenfeld ,  C. Wayne Bardin

    IPC分类号： C07K14/47 ,  C12N1/00 ,  C12N5/10 ,  C12N15/11 ,  C12N15/63

    CPC分类号： C07K14/4702

    摘要： Disclosed is a POU transcription factor, Sprm-1 and analogs thereof, DNA molecules encoding the Sprm-1 proteins, and chimeric constructs, vectors and host cells containing the DNA molecules. Also disclosed are methods to identify putative male contraceptive agents. One involves the steps of isolating a DNA regulatory region responsive to Sprm-1, stably transforming a heterologous cell line with a first chimeric DNA construct containing a constitutive promoter capable of functioning in the cell line operably linked to a Sprm-1 DNA and a second chimeric DNA construct containing the DNA regulatory region operably linked to a reporter gene. The thus-transformed cell line is cultural in media containing the compound so that the thus-cultured cell line can be assayed to determine the Sprm-1 inhibitory activity of the compound. The above method can be modified to evaluate the Sprm-1 inhibitory activity of DNA molecules of interest, by transiently transfecting the thus-transformed cell line with a third DNA construct containing a constitutive promoter capable of functioning in the cell line operably linked to the DNA molecule, and then culturing the thus-transfected cell line so that it can be assayed to determine the inhibitory activity of the DNA molecule. Antibodies specific to Sprm-1, and their use in diagnosing male germ cell Sprm-1-mediated diseases or conditions are further disclosed.

    摘要翻译： 公开了POU转录因子，Sprm-1及其类似物，编码Sprm-1蛋白的DNA分子，以及含有DNA分子的嵌合构建体，载体和宿主细胞。 还公开了鉴定推定的男性避孕药的方法。 一个涉及以下步骤：分离针对Sprm-1的DNA调节区，用含有可在与Sprm-1 DNA可操作连接的细胞系中起作用的组成型启动子的第一嵌合DNA构建体稳定转化异源细胞系， 含有可操作地连接到报道基因的DNA调控区的嵌合DNA构建体。 这样转化的细胞系是含有化合物的培养基中的培养物，因此可以测定这样培养的细胞系以确定化合物的Sprm-1抑制活性。 可以修饰上述方法以通过用含有可操作地连接到DNA的细胞系中起作用的组成型启动子的第三种DNA构建体瞬时转染由此转化的细胞系来评价感兴趣的DNA分子的Sprm-1抑制活性 分子，然后培养如此转染的细胞系，使其可以被测定以确定DNA分子的抑制活性。 进一步公开了对Sprm-1特异性的抗体及其在诊断雄性生殖细胞Sprm-1介导的疾病或病症中的用途。

    Human inhibin
    7.
    发明授权
    Human inhibin 失效
    人抑制素

    公开(公告)号：US4737578A

    公开(公告)日：1988-04-12

    申请号：US848924

    申请日：1986-04-07

    申请人： Ronald M. Evans ,  Michael G. Rosenfeld ,  Gail Cerelli ,  Kelly E. Mayo ,  Joachim Spiess ,  Jean E. F. Rivier ,  Wylie W. Vale, Jr.

    发明人： Ronald M. Evans ,  Michael G. Rosenfeld ,  Gail Cerelli ,  Kelly E. Mayo ,  Joachim Spiess ,  Jean E. F. Rivier ,  Wylie W. Vale, Jr.

    IPC分类号： A61K38/00 ,  C07K14/575 ,  C07K7/10 ,  C07K13/00

    CPC分类号： C07K14/575 ,  A61K38/00 ,  Y10S930/26

    摘要： Proteins with inhibin activity having a weight of about 32,000 daltons. The molecule is composed of two chains having molecular weights of about 18,000 and about 14,000 daltons, respectively, which are bound together by disulfide bonding. The 18K chain is obtained from the human inhibin gene and has the formula: H-Ser-Thr-Pro-Leu-Met-Ser-Trp-Pro-Trp-Ser-Pro-Ser-Ala-Leu-Arg-Leu-Leu-Gln-Arg-Pro-Pro-Glu-Glu-Pro-Ala-Ala-His-Ala-Asn-Cys-His-Arg-Val-Ala-Leu-Asn-Ile-Ser-Phe-Gln-Glu-Leu-Gly-Trp-Glu-Arg-Trp-Ile-Val-Tyr-Pro-Pro-Ser-Phe-R.sub.65 -Phe-His-Tyr-Cys-His-Gly-Gly-Cys-Gly-Leu-His-Ile-Pro-Pro-Asn-Leu-Ser-Leu-Pro-Val-Pro-Gly-Ala-Pro-Pro-Thr-Pro-Ala-Gln-Pro-Tyr-Ser-Leu-Leu-Pro-Gly-Ala-Gln-Pro-Cys-Cys-Ala-Ala-Leu-Pro-Gly-Thr-Met-Arg-Pro-Leu-His-Val-Arg-Thr-Thr-Ser-Asp-Gly-Gly-Tyr-Ser-P e-Lys-Tyr-Glu-Thr-Val-Pro-Asn-Leu-Leu-Thr-Gln-His-Cys-Ala-Cys-Ile-OH, wherein R.sub.65 is Ile or Arg. The 18K chain is connected by disulfide bonding to the 14K chain.

    摘要翻译： 具有约32,000道尔顿重量的具有抑制素活性的蛋白质。 该分子由分子量分别为约18,000和约14,000道尔顿的两条链组成，它们通过二硫键结合在一起。 18K链由人抑制素基因得到，具有下式：H-Ser-Thr-Pro-Leu-Met-Ser-Trp-Pro-Trp-Ser-Pro-Ser-Ala-Leu-Arg-Leu-Leu Gln-Arg-Pro-Pro-Glu-Glu-Pro-Ala-Ala-His-Ala-Asn-Cys-His-Arg-Val-Ala-Le-Asn-Ile-Ser-Phe-Gln-Glu- Leu-Gly-Trp-Glu-Arg-Trp-Ile-Val-Tyr-Pro-Pro-Ser-Phe-R65-Phe-His-Tyr-Cys-His-Gly-Gly-Cys-Gly-Leu-His- Ile-Pro-Pro-As n-Leu-Ser-Leu-Pro-Val-Pro-Gly-Ala-Pro-Pro-Thr-Pro-Ala-Gln-Pro-Tyr-Ser-Leu-Leu-Pro-Gly -Ala-Gln-Pro-Cys-Cys-Ala-Ala-Leu-Pro-Gly-Thr-Met-Arg-Pr-Leu-His-Val-Arg-Thr-Thr-Ser-Asp-Gly-Gly- Tyr-Ser-P e-Lys-Tyr-Glu-Thr-Val-Pro-Asn-Leu-Leu-Thr-Gln-His-Cys-Ala-Cys-Ile-OH，其中R65是Ile或Arg。 18K链通过二硫键与14K链连接。

    DNA encoding human CGRP
    8.
    发明授权
    DNA encoding human CGRP 失效
    编码人CGRP的DNA

    公开(公告)号：US4736023A

    公开(公告)日：1988-04-05

    申请号：US707946

    申请日：1985-03-04

    申请人： Ronald M. Evans ,  Michael G. Rosenfeld

    发明人： Ronald M. Evans ,  Michael G. Rosenfeld

    IPC分类号： A61K38/00 ,  C07K14/575 ,  C07H15/12

    CPC分类号： C07K14/57527 ,  A61K38/00

    摘要： Human CGRP (calcitonin-gene-related peptide) has the formula: H-Ala-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asn-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH.sub.2. Human CGRP or pharmaceutically acceptable salts thereof, dispersed in a pharmaceutically acceptable liquid or solid carrier, can be administered to mammals, including humans, to influence memory, mood and pain appreciation and to achieve a substantial lowering of blood pressure or gastric acid secretion over an extended period of time. They also may be administered to affect ingestion behavior, taste and sensory perception. By using human CGRP to generate production of antibodies, it should be possible to diagnose human medullary thyroid carcinoma via immunoassay techniques.

    摘要翻译： 人CGRP（降钙素基因相关肽）具有下式：H-Ala-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser- Arg-Ser-Gly-Gly-Val-Val-Lys-Asn-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Ly-Ala-Phe-NH2。 分散在药学上可接受的液体或固体载体中的人CGRP或其药学上可接受的盐可以被给予包括人在内的哺乳动物，以影响记忆，情绪和疼痛的升高，并且能够实现血压或胃酸分泌的显着降低 延长一段时间 它们也可以被施用以影响摄取行为，味觉和感觉知觉。 通过使用人CGRP产生抗体，应该可以通过免疫测定技术诊断人甲状腺髓样癌。