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公开(公告)号：US5912171A
公开(公告)日：1999-06-15
申请号：US663808
申请日：1996-06-14
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of a human amino acid transporter protein termed EAAT4 and genes encoding such a transporter. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to this transporter gene. Also provided are recombinant expression constructs capable of expressing this amino acid transporter gene in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter protein encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.
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公开(公告)号：US5882926A
公开(公告)日：1999-03-16
申请号：US948569
申请日：1997-10-10
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： This invention relates to novel mammalian excitatory amino acid transporter proteins and genes encoding such proteins. The invention is directed towards the isolation, characterization and use of human excitatory amino acid transporter proteins for pharmacological screening of analogues, agonists, antagonists, inhibitors, modulators and facilitators of excitatory amino acid transport in a variety of tissues, particularly neuronal tissues. This invention provides isolated nucleic acid encoding a novel excitatory amino acid transporter subtype that is specifically expressed in retina. Also provided are recombinant expression constructs capable of expressing this novel transporter in transformed prokaryotic and eukaryotic cells, and also provides such transformed cell cultures producing the novel human transporter. Purified transporter protein and membranes comprising the transporter protein are also provided. The invention provides methods of screening compounds in vitro for transporter binding properties using said preparations of protein and membranes from transformed cell cultures, as well as from amphibian oocytes expressing the human transporter protein provided herein.
摘要翻译： 本发明涉及新型的哺乳动物兴奋性氨基酸转运蛋白和编码这种蛋白质的基因。 本发明涉及人类兴奋性氨基酸转运蛋白的分离，表征和用途，用于药物筛选各种组织，特别是神经元组织中兴奋性氨基酸转运的类似物，激动剂，拮抗剂，抑制剂，调节剂和促进剂。 本发明提供编码在视网膜中特异性表达的新型兴奋性氨基酸转运子亚型的分离的核酸。 还提供了能够在转化的原核和真核细胞中表达该新型转运蛋白的重组表达构建体，并且还提供产生新型人转运蛋白的转化细胞培养物。 还提供了纯化的转运蛋白和包含转运蛋白的膜。 本发明提供了使用所述来自转化细胞培养物的蛋白质和膜的制备以及来自表达本文提供的人类转运蛋白的两栖动物卵母细胞的体外筛选化合物的转运蛋白结合特性的方法。
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3.发明授权Synthetic calcitonin-gene-related peptides for lowering blood pressure or gastric acid secretion in mammals 失效用于降低哺乳动物血压或胃酸分泌的合成降钙素基因相关肽
公开(公告)号：US4530838A
公开(公告)日：1985-07-23
申请号：US512421
申请日：1983-07-08
申请人： Ronald M. Evans , Larry Swanson , Wylie W. Vale, Jr. , Michael G. Rosenfeld , Susan G. Amara , Marvin R. Brown , Jean E. F. Rivier
发明人： Ronald M. Evans , Larry Swanson , Wylie W. Vale, Jr. , Michael G. Rosenfeld , Susan G. Amara , Marvin R. Brown , Jean E. F. Rivier
IPC分类号： A61K38/00 , C07K14/575 , A61K37/02 , C07C103/52
CPC分类号： C07K14/57527 , A61K38/00
摘要： CGRP (calcitonin-gene-related peptide) has the formula: ##STR1## A related peptide has the residue Lys instead of Glu in the 35-position. CGRP or this homolog or pharmaceutically acceptable salts thereof, dispersed in a pharmaceutically acceptable liquid or solid carrier, can be administered to mammals, including humans, to influence memory, mood and pain appreciation and to achieve a substantial lowering of blood pressure or gastric acid secretion over an extended period of time. They also may be administered to affect ingestion behavior, taste and sensory perception.
摘要翻译： CGRP（降钙素基因相关肽）具有以下分子式：其中，ArgLeuAlaGlyLeuLeuSerArgSerGly GlyValValLysAspAsnPheValProThr + TR AsnValGlySerGluAlaPheNH2。 相关肽在35位具有残基Lys而不是Glu。 分散在药学上可接受的液体或固体载体中的CGRP或其同系物或其药学上可接受的盐可以施用于包括人在内的哺乳动物，以影响记忆，情绪和疼痛升高，并实现血压或胃酸分泌的显着降低 在很长一段时间内。 它们也可以被施用以影响摄取行为，味觉和感觉知觉。
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公开(公告)号：US06458571B1
公开(公告)日：2002-10-01
申请号：US09042709
申请日：1998-03-17
申请人： Susan G. Amara , Jeffrey L. Arriza
发明人： Susan G. Amara , Jeffrey L. Arriza
IPC分类号： C12N900
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of the human amino acid transporter proteins EAAT1, EAAT2, EAAT3 and ASCT1. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to each of these transporter genes. Also provided are recombinant expression constructs capable of expressing each of the amino acid transporter genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter proteins encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.
摘要翻译： 本发明涉及新型哺乳动物氨基酸转运蛋白和编码这些蛋白质的基因。 本发明涉及人氨基酸转运蛋白EAAT1，EAAT2，EAAT3和ASCT1的分离，表征和药理学应用。 本发明特别提供了与这些转运蛋白基因中的每一个相对应的mRNA的分离的互补DNA拷贝。 还提供了能够在转化的原核和真核细胞的培养物中表达本发明的每个氨基酸转运蛋白基因以及合成其中编码的人氨基酸转运蛋白的转化细胞的这种培养物的重组表达构建体。 本发明还提供了使用来自用重组表达构建体转化的细胞的培养物的转运蛋白的制备物筛选具有转运调节特性的体外化合物的方法。
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5.发明授权公开(公告)号：US06284505B1
公开(公告)日：2001-09-04
申请号：US09397238
申请日：1999-09-16
IPC分类号： C12N900
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： This invention relates to novel mammalian excitatory amino acid transporter (EAAT) proteins and genes encoding such proteins. The invention is directed towards the isolation, characterization and use of human excitatory amino acid transporter proteins for pharmacological screening of analogues, agonists, antagonists, inhibitors, modulators and facilitators of excitatory amino acid transport in a variety of tissues, particularly neuronal tissues. This invention provides isolated nucleic acid encoding a novel excitatory amino acid transporter subtype that is specifically expressed in retina. Also provided are recombinant expression constructs capable of expressing this novel transporter in transformed prokaryotic and eukaryotic cells, and also provides such transformed cell cultures producing the novel human transporter. Purified transporter protein and membranes comprising the transporter protein are also provided. The invention provides methods of screening compounds in vitro for transporter binding properties using said preparations of protein and membranes from transformed cell cultures, as well as from amphibian oocytes expressing the human transporter protein provided herein.
摘要翻译： 本发明涉及新型哺乳动物兴奋性氨基酸转运蛋白（EAAT）蛋白和编码这种蛋白质的基因。 本发明涉及人类兴奋性氨基酸转运蛋白的分离，表征和用途，用于药物筛选各种组织，特别是神经元组织中兴奋性氨基酸转运的类似物，激动剂，拮抗剂，抑制剂，调节剂和促进剂。 本发明提供编码在视网膜中特异性表达的新型兴奋性氨基酸转运子亚型的分离的核酸。 还提供了能够在转化的原核和真核细胞中表达该新型转运蛋白的重组表达构建体，并且还提供产生新型人转运蛋白的转化细胞培养物。 还提供了纯化的转运蛋白和包含转运蛋白的膜。 本发明提供了使用所述来自转化细胞培养物的蛋白质和膜的制备以及来自表达本文提供的人类转运蛋白的两栖动物卵母细胞的体外筛选化合物的转运蛋白结合特性的方法。
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公开(公告)号：US6060307A
公开(公告)日：2000-05-09
申请号：US332740
申请日：1999-06-14
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of a human amino acid transporter protein termed EAAT4 and genes encoding such a transporter. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to this transporter gene. Also provided are recombinant expression constructs capable of expressing this amino acid transporter gene in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter protein encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.
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公开(公告)号：US5989825A
公开(公告)日：1999-11-23
申请号：US188469
申请日：1998-11-09
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： This invention relates to novel mammalian excitatory amino acid transporter proteins and genes encoding such proteins. The invention is directed towards the isolation, characterization and use of human excitatory amino acid transporter proteins for pharmacological screening of analogues, agonists, antagonists, inhibitors, modulators and facilitators of excitatory amino acid transport in a variety of tissues, particularly neuronal tissues. This invention provides isolated nucleic acid encoding a novel excitatory amino acid transporter subtype that is specifically expressed in retina. Also provided are recombinant expression constructs capable of expressing this novel transporter in transformed prokaryotic and eukaryotic cells, and also provides such transformed cell cultures producing the novel human transporter. Purified transporter protein and membranes comprising the transporter protein are also provided. The invention provides methods of screening compounds in vitro for transporter binding properties using said preparations of protein and membranes from transformed cell cultures, as well as from amphibian oocytes expressing the human transporter protein provided herein.
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公开(公告)号：US5919628A
公开(公告)日：1999-07-06
申请号：US42929
申请日：1998-03-17
申请人： Susan G. Amara , Jeffrey L. Arriza
发明人： Susan G. Amara , Jeffrey L. Arriza
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of the human amino acid transporter proteins EAAT1, EAAT2, EAAT3 and ASCT1. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to each of these transporter genes. Also provided are recombinant expression constructs capable of expressing each of the amino acid transporter genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter proteins encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.
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公开(公告)号：US5776774A
公开(公告)日：1998-07-07
申请号：US546666
申请日：1995-10-23
申请人： Susan G. Amara , Jeffrey L. Arriza
发明人： Susan G. Amara , Jeffrey L. Arriza
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of the human amino acid transporter proteins EAAT1, EAAT2, EAAT3 and ASCT1. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to each of these transporter genes. Also provided are recombinant expression constructs capable of expressing each of the amino acid transporter genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter proteins encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.
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公开(公告)号：US06333400B1
公开(公告)日：2001-12-25
申请号：US09368282
申请日：1999-08-03
IPC分类号： C07H2104
CPC分类号： C07K14/47 , A61K38/00 , C07K2319/00 , C12N2799/023
摘要： The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of a human amino acid transporter protein termed EAAT4 and genes encoding such a transporter. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to this transporter gene. Also provided are recombinant expression constructs capable of expressing this amino acid transporter gene in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter protein encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.
摘要翻译： 本发明涉及新型哺乳动物氨基酸转运蛋白和编码这些蛋白质的基因。 本发明涉及被称为EAAT4的人氨基酸转运蛋白和编码这种转运蛋白的基因的分离，表征和药理学应用。 本发明具体提供了与该转运蛋白基因相对应的mRNA的分离的互补DNA拷贝。 还提供了能够在转化的原核和真核细胞的培养物中表达该氨基酸转运蛋白基因的重组表达构建体，以及合成其中编码的人氨基酸转运蛋白的转化细胞的这种培养物。 本发明还提供了使用来自用重组表达构建体转化的细胞的培养物的转运蛋白的制备物筛选具有转运调节特性的体外化合物的方法。
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