公开(公告)号：US06960470B1

公开(公告)日：2005-11-01

申请号：US09353126

申请日：1999-07-14

申请人： Roberto Malinow ,  Shahid Zaman ,  Sangram S. Sisodia ,  David R. Borchelt ,  Michael K. Lee

发明人： Roberto Malinow ,  Shahid Zaman ,  Sangram S. Sisodia ,  David R. Borchelt ,  Michael K. Lee

IPC分类号： C12N5/02 ,  C12N5/06 ,  G01N33/50 ,  G01N33/68

CPC分类号： G01N33/6896 ,  G01N33/5058 ,  G01N2333/4709 ,  G01N2800/2821

摘要： Methods of screening candidate drugs for the treatment of Alzheimer's disease are provided. Employing tissue slices of mutant mouse hippocampal cells having an extra copy of a mutant form of the presenilin-1 gene, it is found that these cells have enhanced potentiation to tetanic stimuli. By subjecting both wild-type and mutant hippocampal cells to tetanic stimuli in the presence of a candidate drug, reduction of the enhanced potentiation of the mutant cells without significant change in the potentiation of the wild-type cells indicates a successful candidate.

摘要翻译： 提供了筛选候选药物治疗阿尔茨海默病的方法。 使用具有突变体形式的早老蛋白-1基因的额外拷贝的突变小鼠海马细胞的组织切片，发现这些细胞对强直性刺激具有增强的增强作用。 通过在候选药物的存在下将野生型和突变型海马细胞同时进行破伤风刺激，不增加野生型细胞增强的突变体细胞的增强增强的减少表明是成功的候选者。

公开(公告)号：US06362288B1

公开(公告)日：2002-03-26

申请号：US09616625

申请日：2000-07-26

申请人： Christopher Michael Brewer ,  Igor Chorvath ,  Michael K. Lee ,  Yongjun Lee ,  Dawei Li ,  Koji Nakanishi ,  Robert Leo Oldinski ,  Lenin James Petroff ,  Richard L. Rabe ,  David Joseph Romenesko

发明人： Christopher Michael Brewer ,  Igor Chorvath ,  Michael K. Lee ,  Yongjun Lee ,  Dawei Li ,  Koji Nakanishi ,  Robert Leo Oldinski ,  Lenin James Petroff ,  Richard L. Rabe ,  David Joseph Romenesko

IPC分类号： G08G7708

CPC分类号： C08L77/02 ,  C08G77/12 ,  C08G77/14 ,  C08G77/16 ,  C08G77/18 ,  C08G77/20 ,  C08G77/26 ,  C08G77/44 ,  C08G77/445 ,  C08G77/448 ,  C08G77/455 ,  C08G77/458 ,  C08G77/46 ,  C08L77/00 ,  C08L77/06 ,  C08L83/04 ,  Y10S525/903 ,  C08L83/00 ,  C08L2666/14

摘要： A method for preparing a thermoplastic elastomer is disclosed, said method comprising (I) mixing (A) a rheologically stable polyamide resin having a melting point or glass transition temperature of 25° C. to 275° C., (B) a silicone base comprising (B′) 100 parts by weight of a diorganopolysiloxane gum having a plasticity of at least 30 and having an average of at least 2 alkenyl groups in its molecule and (B″) 5 to 200 parts by weight of a reinforcing filler, the weight ratio of said silicone base to said polyamide resin being greater than 35:65 to 85:15, (C) a compatibilizer selected from (i) a coupling agent, (ii) a functional diorganopolysiloxane or (iii) a copolymer comprising at least one diorganopolysiloxane block and at least one block selected from polyamide, polyether, polyurethane, polyurea, polycarbonate or polyacrylate, (D) an organohydrido silicon compound which contains an average of at least 2 silicon-bonded hydrogen groups in its molecule and (E) a hydrosilation catalyst, components (D) and (E) being present in an amount sufficient to cure said diorganopolysiloxane (B′); and (II) dynamically curing said diorganopolysiloxane (B′).

摘要翻译： 公开了一种制备热塑性弹性体的方法，所述方法包括（I）混合（A）熔点或玻璃化转变温度为25℃至275℃的流变学稳定的聚酰胺树脂，（B）硅氧烷基 包含（B'）100重量份的可塑性为至少30且在其分子中平均具有至少2个烯基的二有机聚硅氧烷胶，（B“）为（B”）5〜200重量份的增强填料， 所述硅氧烷基与所述聚酰胺树脂的重量比大于35:65至85:15，（C）选自（i）偶联剂，（ii）官能二有机聚硅氧烷或（iii）共聚物的增容剂， 至少一个二有机聚硅氧烷嵌段和至少一个选自聚酰胺，聚醚，聚氨酯，聚脲，聚碳酸酯或聚丙烯酸酯的嵌段;（D）在其分子中平均含有至少2个与硅键合的氢基团的有机氢化硅化合物，（E） 硅氢化催化剂 部分（D）和（E）以足以固化所述二有机聚硅氧烷（B'）的量存在; 和（II）动态固化所述二有机聚硅氧烷（B'）。

公开(公告)号：US06362287B1

公开(公告)日：2002-03-26

申请号：US09535556

申请日：2000-03-27

申请人： Igor Chorvath ,  Michael K. Lee ,  Yongjun Lee ,  Dawei Li ,  Koji Nakanishi ,  Robert Leo Oldinski ,  Richard L. Rabe ,  David Joseph Romenesko ,  Jeffrey Paul Sage

发明人： Igor Chorvath ,  Michael K. Lee ,  Yongjun Lee ,  Dawei Li ,  Koji Nakanishi ,  Robert Leo Oldinski ,  Richard L. Rabe ,  David Joseph Romenesko ,  Jeffrey Paul Sage

IPC分类号： C08G7708

CPC分类号： C08L83/04 ,  C08G77/12 ,  C08G77/20 ,  C08G77/44 ,  Y10S525/903 ,  C08L2666/14 ,  C08L2666/28

摘要： A method for preparing a thermoplastic elastomer is disclosed, said method comprising (I) mixing (A) a rheologically stable polyamide resin having a melting point or glass transition temperature of 25° C. to 275° C., (B) a silicone base comprising (B′) 100 parts by weight of a diorganopolysiloxane gum having a plasticity of at least 30 and having an average of at least 2 alkenyl radicals in its molecule and (B″) 5 to 200 parts by weight of a reinforcing filler,  the weight ratio of said silicone base to said polyamide resin being greater than 35:65 to 85:15, (C) 0.1 to 5 parts by weight of a hindered phenol compound for each 100 parts by weight of said polyamide and said silicone base, (D) an organohydrido silicon compound which contains an average of at least 2 silicon-bonded hydrogen groups in its molecule and (E) a hydrosilation catalyst, components (D) and (E) being present in an amount sufficient to cure said diorganopolysiloxane (B′); and (II) dynamically curing said diorganopolysiloxane (B′), wherein at least one property of the thermoplastic elastomer selected from tensile strength or elongation is at least 25% greater than the respective property for a corresponding simple blend wherein said diorganopolysiloxane is not cured and said thermoplastic elastomer has an elongation of at least 25%.

摘要翻译： 公开了一种制备热塑性弹性体的方法，所述方法包括（I）混合（A）熔点或玻璃化转变温度为25℃至275℃的流变学稳定的聚酰胺树脂，（B）硅氧烷基 包含（B'）100重量份的塑性为至少30的二有机聚硅氧烷胶，其分子中平均具有至少2个烯基，（B“）为5〜200重量份的增强填料， 所述硅氧烷基与所述聚酰胺树脂的重量比大于35:65至85:15，（C）每100重量份所述聚酰胺和所述有机硅基料为0.1至5重量份的受阻酚化合物， （D）在其分子中平均含有至少2个与硅键合的氢基团的有机氢化硅化合物，（E）硅氢化催化剂，组分（D）和（E）以足以固化所述二有机聚硅氧烷的量存在 B'）; 和（II）动态固化所述二有机聚硅氧烷（B'），其中选自拉伸强度或伸长率的热塑性弹性体的至少一种性能比相应的简单混合物的相应性能大至少25％，其中所述二有机聚硅氧烷不固化， 所述热塑性弹性体的伸长率至少为25％。